FastQCFastQC Report
Sat 18 Jun 2016
SRR3554315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024831
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48050.4688577921628054No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33940.3311765549636964No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20660.20159421407041747No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA12180.11884886386145617No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT11510.11231120057843683No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG253.8905084E-545.0000041
TTAACGA253.8905084E-545.00000422
ACGTATA253.8905084E-545.00000420
AATCGCG253.8905084E-545.0000041
CTATACG302.1652704E-644.9999961
TTAGCCG850.042.352941
CGGGTTA800.042.18756
TGCGGGT800.042.18754
ACGGGTC1350.041.6666685
ACGGGTA2650.041.6037755
ATATCCG1200.041.2499961
AATTGCG603.6379788E-1241.2499961
TATCGCG501.0804797E-940.5000041
TAGCGGA451.9279469E-840.03
TATAGCG451.9279469E-840.01
CGTAACG451.9279469E-840.01
TTACGGG4450.039.43823
ACGGGAT16050.039.3925255
CGGATGT403.4577351E-739.3756
ATAACGG2250.039.02
Produced by FastQC (version 0.11.5)