##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1024831
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35418717817865	34.0	31.0	34.0	30.0	34.0
2	32.52471578240705	34.0	31.0	34.0	31.0	34.0
3	32.62245482425883	34.0	31.0	34.0	31.0	34.0
4	36.11801848304745	37.0	35.0	37.0	35.0	37.0
5	35.961205310924434	37.0	35.0	37.0	35.0	37.0
6	35.93832251366323	37.0	35.0	37.0	35.0	37.0
7	35.97132112514161	37.0	35.0	37.0	35.0	37.0
8	36.08702605600338	37.0	35.0	37.0	35.0	37.0
9	37.72606117496446	39.0	38.0	39.0	35.0	39.0
10	37.34111185161261	39.0	37.0	39.0	34.0	39.0
11	37.33562606907871	39.0	37.0	39.0	34.0	39.0
12	37.31447428893154	39.0	37.0	39.0	34.0	39.0
13	37.294770552413034	39.0	37.0	39.0	34.0	39.0
14	38.58132609181416	40.0	38.0	41.0	34.0	41.0
15	38.58745002834614	40.0	38.0	41.0	34.0	41.0
16	38.572911045821215	40.0	38.0	41.0	34.0	41.0
17	38.53245461934699	40.0	38.0	41.0	34.0	41.0
18	38.47006189313165	40.0	38.0	41.0	34.0	41.0
19	38.48108419827269	40.0	38.0	41.0	34.0	41.0
20	38.39638145216138	40.0	38.0	41.0	34.0	41.0
21	38.34204956719693	40.0	38.0	41.0	34.0	41.0
22	38.2794509533767	40.0	38.0	41.0	34.0	41.0
23	38.21004438780638	40.0	37.0	41.0	34.0	41.0
24	38.179885268888235	40.0	37.0	41.0	34.0	41.0
25	38.11854539919265	40.0	37.0	41.0	33.0	41.0
26	38.043442284630345	40.0	37.0	41.0	33.0	41.0
27	37.976220469521316	40.0	37.0	41.0	33.0	41.0
28	37.88727897575308	40.0	37.0	41.0	33.0	41.0
29	37.81739330679888	40.0	37.0	41.0	33.0	41.0
30	37.795339914581035	40.0	37.0	41.0	33.0	41.0
31	37.72526982497602	40.0	37.0	41.0	33.0	41.0
32	37.66703290591327	40.0	37.0	41.0	33.0	41.0
33	37.58869218436991	40.0	36.0	41.0	33.0	41.0
34	37.47436211433885	40.0	36.0	41.0	32.0	41.0
35	37.43325875193081	40.0	36.0	41.0	32.0	41.0
36	37.34633515184455	40.0	36.0	41.0	32.0	41.0
37	37.28488111698417	40.0	36.0	41.0	32.0	41.0
38	37.20922864355196	39.0	36.0	41.0	32.0	41.0
39	37.15768355953323	39.0	35.0	41.0	31.0	41.0
40	37.05364982128761	39.0	35.0	41.0	31.0	41.0
41	36.96382232777892	39.0	35.0	41.0	31.0	41.0
42	36.90662070136442	39.0	35.0	41.0	31.0	41.0
43	36.84776611948702	39.0	35.0	41.0	31.0	41.0
44	36.74312740344506	39.0	35.0	41.0	31.0	41.0
45	36.61015621112164	39.0	35.0	41.0	30.0	41.0
46	36.46944325454636	39.0	35.0	41.0	30.0	41.0
47	36.372312117802835	39.0	35.0	40.0	30.0	41.0
48	36.26775439072394	39.0	35.0	40.0	30.0	41.0
49	36.16021861165402	39.0	35.0	40.0	30.0	41.0
50	36.05666300102163	38.0	35.0	40.0	29.0	41.0
51	35.04890562443954	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	14.0
12	12.0
13	8.0
14	37.0
15	52.0
16	85.0
17	146.0
18	253.0
19	429.0
20	722.0
21	1072.0
22	1662.0
23	2420.0
24	3372.0
25	4170.0
26	5586.0
27	7124.0
28	9001.0
29	11443.0
30	14979.0
31	19403.0
32	25499.0
33	34248.0
34	52022.0
35	68673.0
36	83745.0
37	122602.0
38	210169.0
39	345708.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.359161656897577	33.05501102132937	20.98463063666107	25.601196685111983
2	21.476223884718554	32.86434543841863	21.106211658312443	24.553219018550376
3	20.877881328726396	31.283011540439347	21.339908726414404	26.49919840441985
4	22.52898282741252	31.064341340181944	19.768137380699844	26.638538451705696
5	19.175942179735	34.610779728560125	17.18127183896662	29.032006252738256
6	20.4105847695864	37.5917590314891	19.477455307265295	22.520200891659208
7	84.56916311079583	6.58908639570817	4.602124642989917	4.2396258505060835
8	85.97563891022033	5.955225788447071	4.3674518042487005	3.7016834970839096
9	76.29013954495912	11.619281618139967	7.4692315123176405	4.621347324583272
10	31.371513937419927	41.479131681223535	14.103496088623393	13.045858292733142
11	23.921895414951344	29.157295202818805	29.44944093221224	17.471368450017614
12	27.348314014700957	24.816677091149664	29.28209626757973	18.552912626569647
13	23.575496838015244	26.89936194357899	28.748642459098132	20.776498759307632
14	19.663925076427237	28.556903528484206	29.372062320519188	22.407109074569366
15	19.408273168942	30.264502147183293	29.34005704355157	20.987167640323136
16	21.468027411348796	29.007124101437213	28.76649906179653	20.758349425417457
17	20.158543213466416	30.20800502717033	28.96516596394918	20.668285795414075
18	19.96973159477026	30.234936296813814	28.78289200853604	21.012440099879885
19	21.787299564513564	30.07305594776114	28.19967389745236	19.93997059027293
20	23.76948004109946	28.439518320581637	28.766596638860452	19.024404999458447
21	23.3148685002698	29.424851512102972	27.75296609880068	19.50731388882655
22	21.706017870263487	29.244236366776573	27.767309927197754	21.28243583576219
23	21.199202600233598	29.14392714506099	28.458741002174992	21.198129252530418
24	20.740687976846914	28.2203602350046	30.072275331249738	20.966676456898746
25	20.250070499428684	31.71644885839714	26.980546060765143	21.052934581409033
26	21.07771915564615	31.234027854348668	27.785556838151855	19.90269615185333
27	20.581344631456307	30.339538909342124	27.440134031855006	21.638982427346555
28	21.42704504450002	30.08447246424045	28.11673339311555	20.371749098143987
29	20.60876378641942	30.14087200718948	27.327920408340496	21.922443798050605
30	20.328815190016694	29.955085277475018	27.9629519403687	21.753147592139584
31	21.065522022655443	30.169559663983627	27.402664439307557	21.36225387405338
32	21.951619340164378	29.83848068608385	27.296890902012137	20.913009071739634
33	20.42229401725748	31.179872583869926	27.36851246693357	21.029320931939022
34	19.88103404366183	30.349101461606843	28.556903528484206	21.212960966247117
35	22.205612437562877	28.799089801147705	28.45112999118879	20.544167770100632
36	20.958187252337215	31.376978252999766	27.63148265421323	20.033351840449793
37	20.74868929608882	32.222581088979545	27.397004969599863	19.631724645331765
38	19.82443934658495	30.09140043577917	29.543017336516947	20.541142881118933
39	20.935061488186832	30.091595589907016	27.487849216114657	21.48549370579149
40	21.629224720953992	29.057278712295005	29.576486269443446	19.737010297307556
41	18.757824460813538	28.87461444862616	31.53700463783785	20.830556452722448
42	19.13632589178118	29.900930006996273	28.865832512872853	22.096911588349688
43	20.541728343502488	29.357718492122114	28.746788494883546	21.35376466949185
44	20.444053702512903	29.41255680204834	28.407903351869724	21.735486143569037
45	20.237483058182278	30.251719551809032	28.119953436225092	21.3908439537836
46	18.682787698654703	30.18712353549024	29.147439919362316	21.98264884649274
47	20.201379544529782	29.519306109983013	28.953359139214175	21.325955206273033
48	20.69150913662838	28.155666641621885	29.597075029931762	21.55574919181797
49	18.718988789371124	30.825765418883698	28.53836388633833	21.916881905406843
50	18.977958317029834	29.58419485749358	29.28834119967097	22.14950562580562
51	19.010061171061373	28.826704110238666	29.317419164720814	22.845815553979143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6081.0
1	4809.0
2	3537.0
3	2505.0
4	1473.0
5	1481.0
6	1489.0
7	1642.0
8	1795.0
9	1894.0
10	1993.0
11	2311.5
12	2630.0
13	2777.0
14	2924.0
15	3338.0
16	3752.0
17	3807.5
18	3863.0
19	4126.0
20	4389.0
21	5344.5
22	6300.0
23	8956.0
24	11612.0
25	12348.0
26	15450.0
27	17816.0
28	20875.0
29	23934.0
30	27784.5
31	31635.0
32	35265.5
33	38896.0
34	44442.0
35	49988.0
36	50562.0
37	51136.0
38	53457.5
39	55779.0
40	59497.5
41	63216.0
42	64334.0
43	65452.0
44	67103.0
45	68754.0
46	70087.5
47	71421.0
48	69559.5
49	67698.0
50	64752.0
51	61806.0
52	57409.0
53	53012.0
54	47919.0
55	42826.0
56	40423.5
57	38021.0
58	36426.5
59	34832.0
60	34941.0
61	35050.0
62	31699.0
63	28348.0
64	24331.5
65	20315.0
66	16791.5
67	13268.0
68	11378.5
69	9489.0
70	7897.0
71	6305.0
72	5314.0
73	4323.0
74	3627.5
75	2269.0
76	1606.0
77	1290.5
78	975.0
79	759.5
80	544.0
81	390.5
82	237.0
83	208.5
84	180.0
85	107.5
86	35.0
87	36.5
88	38.0
89	31.5
90	25.0
91	16.0
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	2.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1024831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.179907870088794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68904743606271	17.08105330602536
2	8.36778449742326	3.879289474540568
3	3.3478536954765277	2.3280882067104667
4	1.8612017338257663	1.7256993887092316
5	1.27701379816888	1.4800531095193403
6	0.9648376857057698	1.3418909198549658
7	0.7754167830435449	1.2581862714309
8	0.6528935712410555	1.2107210264272736
9	0.5825968034491512	1.2154086206423598
>10	6.98254096238707	36.307252988659855
>50	1.132050646843616	18.2338916005549
>100	0.3595626628526622	11.83262132689983
>500	0.0050821577788362155	0.8655645159731508
>1k	0.0021175657411817562	1.2402792440518073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4805	0.4688577921628054	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3394	0.3311765549636964	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2066	0.20159421407041747	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1218	0.11884886386145617	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1151	0.11231120057843683	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9515412785132378E-4	0.0	0.0	0.11123785287525455	0.0
2	1.9515412785132378E-4	0.0	0.0	0.1753459838744144	0.0
3	1.9515412785132378E-4	0.0	0.0	0.24696754879585026	0.0
4	1.9515412785132378E-4	0.0	0.0	0.5547256084173878	0.0
5	1.9515412785132378E-4	0.0	0.0	0.6240053238046078	0.0
6	1.9515412785132378E-4	0.0	0.0	1.0783241334424896	0.0
7	2.927311917769857E-4	0.0	0.0	1.3960350535844446	0.0
8	2.927311917769857E-4	0.0	0.0	1.7036955361420565	0.0
9	2.927311917769857E-4	0.0	0.0	2.070975604758248	0.0
10	2.927311917769857E-4	0.0	0.0	2.7120569147498466	0.0
11	2.927311917769857E-4	0.0	0.0	3.225117116870977	0.0
12	2.927311917769857E-4	0.0	0.0	3.4961862004564654	0.0
13	2.927311917769857E-4	0.0	0.0	3.617279336788212	0.0
14	2.927311917769857E-4	0.0	0.0	3.756033921690503	0.0
15	2.927311917769857E-4	0.0	0.0	3.867466928693609	0.0
16	2.927311917769857E-4	0.0	0.0	4.078818849156592	0.0
17	2.927311917769857E-4	0.0	0.0	4.3248106273131866	0.0
18	2.927311917769857E-4	0.0	0.0	4.610321116359673	0.0
19	2.927311917769857E-4	0.0	0.0	4.762248604891928	0.0
20	2.927311917769857E-4	0.0	0.0	4.942571019026552	0.0
21	2.927311917769857E-4	0.0	0.0	5.139383956964612	0.0
22	2.927311917769857E-4	0.0	0.0	5.369177942509546	0.0
23	2.927311917769857E-4	0.0	0.0	5.5938979207303445	0.0
24	3.9030825570264755E-4	0.0	0.0	5.770024521116165	0.0
25	3.9030825570264755E-4	0.0	0.0	5.930148483018176	0.0
26	3.9030825570264755E-4	0.0	0.0	6.085686322915681	0.0
27	3.9030825570264755E-4	0.0	0.0	6.2481521343519075	0.0
28	3.9030825570264755E-4	0.0	0.0	6.423400541162397	0.0
29	3.9030825570264755E-4	0.0	0.0	6.610358195643965	0.0
30	3.9030825570264755E-4	0.0	0.0	6.816831262910665	0.0
31	3.9030825570264755E-4	0.0	0.0	6.995592444022478	0.0
32	3.9030825570264755E-4	0.0	0.0	7.184599216846485	0.0
33	3.9030825570264755E-4	0.0	0.0	7.3709714089444995	0.0
34	3.9030825570264755E-4	0.0	0.0	7.549635012992386	0.0
35	3.9030825570264755E-4	0.0	0.0	7.740007864711353	0.0
36	3.9030825570264755E-4	0.0	0.0	7.951359785174336	0.0
37	3.9030825570264755E-4	0.0	0.0	8.148270300176321	0.0
38	3.9030825570264755E-4	0.0	0.0	8.3698678123515	0.0
39	3.9030825570264755E-4	0.0	0.0	8.577902112641011	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAACG	25	3.8905084E-5	45.000004	1
TTAACGA	25	3.8905084E-5	45.000004	22
ACGTATA	25	3.8905084E-5	45.000004	20
AATCGCG	25	3.8905084E-5	45.000004	1
CTATACG	30	2.1652704E-6	44.999996	1
TTAGCCG	85	0.0	42.35294	1
CGGGTTA	80	0.0	42.1875	6
TGCGGGT	80	0.0	42.1875	4
ACGGGTC	135	0.0	41.666668	5
ACGGGTA	265	0.0	41.603775	5
ATATCCG	120	0.0	41.249996	1
AATTGCG	60	3.6379788E-12	41.249996	1
TATCGCG	50	1.0804797E-9	40.500004	1
TAGCGGA	45	1.9279469E-8	40.0	3
TATAGCG	45	1.9279469E-8	40.0	1
CGTAACG	45	1.9279469E-8	40.0	1
TTACGGG	445	0.0	39.4382	3
ACGGGAT	1605	0.0	39.392525	5
CGGATGT	40	3.4577351E-7	39.375	6
ATAACGG	225	0.0	39.0	2
>>END_MODULE