SRR3554313_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	918298
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4710	0.5129053967230681	No Hit
AATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	3055	0.3326806766430941	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	2314	0.25198791677647125	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGT	2219	0.24164269115254525	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1996	0.21735863521427684	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	1555	0.16933500889689404	TruSeq Adapter, Index 14 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1470	0.16007875439127603	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	945	0.10290777068010601	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	936	0.10192769667362882	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	35	1.2115743E-7	45.000004	1
ACGACTA	35	1.2115743E-7	45.000004	11
GTCGCAC	20	7.032546E-4	45.0	15
TAGCGAA	20	7.032546E-4	45.0	32
TAGGGCC	75	0.0	45.0	5
TCGTAAG	20	7.032546E-4	45.0	1
CGTAACG	25	3.8901904E-5	45.0	1
CACGTTA	25	3.8901904E-5	45.0	43
TATACCG	25	3.8901904E-5	45.0	1
CAATACG	20	7.032546E-4	45.0	1
CTAGCGA	20	7.032546E-4	45.0	31
CCTAGCG	30	2.165023E-6	44.999996	1
GTATACG	30	2.165023E-6	44.999996	1
AATACGC	30	2.165023E-6	44.999996	34
TAGTCCG	30	2.165023E-6	44.999996	1
CCACACG	30	2.165023E-6	44.999996	1
TTACCGG	85	0.0	42.352943	2
AGTACGG	150	0.0	42.0	2
CGATAAG	55	6.002665E-11	40.909092	1
TCGATAG	45	1.927583E-8	40.0	1