##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	918298
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23372696009356	33.0	31.0	34.0	30.0	34.0
2	32.413408283585504	34.0	31.0	34.0	30.0	34.0
3	32.49350102036594	34.0	31.0	34.0	30.0	34.0
4	36.0135555124807	37.0	35.0	37.0	35.0	37.0
5	35.85092529875923	37.0	35.0	37.0	35.0	37.0
6	35.829674027385444	37.0	35.0	37.0	35.0	37.0
7	35.86733500454101	37.0	35.0	37.0	35.0	37.0
8	36.01421434000727	37.0	35.0	37.0	35.0	37.0
9	37.639003896338664	39.0	37.0	39.0	35.0	39.0
10	37.224611182862205	39.0	37.0	39.0	34.0	39.0
11	37.17671823307902	39.0	37.0	39.0	34.0	39.0
12	37.15924569148577	39.0	37.0	39.0	34.0	39.0
13	37.158057623995695	39.0	37.0	39.0	33.0	39.0
14	38.39441662728221	40.0	38.0	41.0	34.0	41.0
15	38.40195884124761	40.0	38.0	41.0	34.0	41.0
16	38.37853942837728	40.0	38.0	41.0	34.0	41.0
17	38.349298375908475	40.0	38.0	41.0	34.0	41.0
18	38.264947762055456	40.0	37.0	41.0	34.0	41.0
19	38.245595656312005	40.0	37.0	41.0	34.0	41.0
20	38.19191155812165	40.0	37.0	41.0	34.0	41.0
21	38.144557649042035	40.0	37.0	41.0	33.0	41.0
22	38.095256659602875	40.0	37.0	41.0	33.0	41.0
23	38.017083778904016	40.0	37.0	41.0	33.0	41.0
24	37.986056813801184	40.0	37.0	41.0	33.0	41.0
25	37.92278432491413	40.0	37.0	41.0	33.0	41.0
26	37.83242259048806	40.0	37.0	41.0	33.0	41.0
27	37.75991780445999	40.0	37.0	41.0	33.0	41.0
28	37.687600321464274	40.0	36.0	41.0	33.0	41.0
29	37.62042931597368	40.0	36.0	41.0	33.0	41.0
30	37.5418448041921	40.0	36.0	41.0	33.0	41.0
31	37.44968408947858	40.0	36.0	41.0	32.0	41.0
32	37.36512548214196	40.0	36.0	41.0	32.0	41.0
33	37.27123657026368	39.0	36.0	41.0	32.0	41.0
34	37.144622987309134	39.0	35.0	41.0	31.0	41.0
35	37.08148770878299	39.0	35.0	41.0	31.0	41.0
36	36.970603224661275	39.0	35.0	41.0	31.0	41.0
37	36.886058773949195	39.0	35.0	41.0	31.0	41.0
38	36.85138484457115	39.0	35.0	41.0	31.0	41.0
39	36.7552134492289	39.0	35.0	41.0	31.0	41.0
40	36.62849096916251	39.0	35.0	41.0	30.0	41.0
41	36.500111075054065	39.0	35.0	41.0	30.0	41.0
42	36.44058573578511	39.0	35.0	41.0	30.0	41.0
43	36.38220054927703	39.0	35.0	40.0	30.0	41.0
44	36.30429228855992	39.0	35.0	40.0	30.0	41.0
45	36.174632853387465	38.0	35.0	40.0	30.0	41.0
46	35.98427090116716	38.0	35.0	40.0	29.0	41.0
47	35.887370984146756	38.0	35.0	40.0	28.0	41.0
48	35.763047507454004	38.0	35.0	40.0	28.0	41.0
49	35.655567147048124	38.0	34.0	40.0	28.0	41.0
50	35.54703592951308	38.0	34.0	40.0	28.0	41.0
51	34.48215176337093	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	11.0
12	10.0
13	17.0
14	17.0
15	52.0
16	69.0
17	152.0
18	249.0
19	492.0
20	875.0
21	1366.0
22	1921.0
23	2752.0
24	3775.0
25	4596.0
26	5679.0
27	7100.0
28	9205.0
29	11862.0
30	15186.0
31	19892.0
32	25651.0
33	34645.0
34	51350.0
35	66254.0
36	82365.0
37	114000.0
38	183419.0
39	275216.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.473854892420544	33.74797723614774	20.072460138212215	25.7057077332195
2	21.862946450934228	32.82757884695382	20.55226081293872	24.757213889173233
3	20.891257522068	30.71453928898898	22.736410184929078	25.657793004013946
4	21.700471960082673	30.85893685927662	20.271415161527088	27.16917601911362
5	18.794116942430453	35.60434630152739	17.321719093366205	28.27981766267595
6	21.32695486650303	36.868097284323824	19.68620208254837	22.118745766624777
7	83.6064109907677	7.252438750819451	4.978993747127839	4.162156511285008
8	85.26077591370121	6.142232695704444	4.0249461503782	4.572045240216139
9	76.25694491330701	11.631736103095074	7.41447765322368	4.696841330374236
10	32.89868866097934	40.33864823837142	14.27728253791253	12.485380562736715
11	23.84106248734071	29.82484988533134	28.778130846413692	17.555956780914254
12	27.18093690719135	25.319558574667482	29.99233364332711	17.507170874814058
13	24.262821001461397	27.060714495730142	27.49902537084911	21.177439131959343
14	19.32858396729602	28.93058680297681	29.31836941820629	22.422459811520877
15	19.594946302834156	31.380662922057983	29.110702625944956	19.91368814916291
16	20.91456150399979	30.65214124390993	28.792287470951695	19.641009781138585
17	20.120592661641428	31.12366573813729	28.862852799418054	19.892888800803224
18	20.379767787798734	32.19151081675012	26.89410191462902	20.53461948082213
19	21.07387797860825	32.21220126799797	27.6743497208967	19.039571032497076
20	23.219368875898674	30.842493395390168	27.568501728197166	18.369636000513996
21	22.103717965192125	31.238007705559635	27.407116208463915	19.25115812078432
22	21.124841826945065	31.436853831762672	27.429549013501063	20.008755327791196
23	21.263576747417506	31.380880716281645	27.10895591627119	20.246586620029664
24	19.913797046274738	31.427488680145228	29.032950088097763	19.62576418548227
25	20.40644758019728	33.037750272787264	25.986880075966624	20.56892207104883
26	21.36103966250607	33.380993969277945	25.389579417574687	19.868386950641295
27	19.524054283032307	32.89313490827596	26.64494532276015	20.93786548593158
28	20.94548828375974	31.849791679825067	28.1399937710852	19.06472626532999
29	21.011588830641035	31.499360773953555	26.336766496278983	21.152283899126427
30	20.907483191730787	33.12269002001529	25.85674802732882	20.113078760925102
31	21.285682861119156	32.98177715730623	25.79990373495314	19.932636246621467
32	22.47462152808783	32.196628981006164	25.41887274065717	19.90987675024883
33	21.92066192020455	33.50927476701463	25.572199874114936	18.99786343866588
34	20.909552236855575	32.05680508941542	27.906627260431797	19.127015413297208
35	23.498689967744678	30.722488778152623	26.959004593280177	18.81981666082252
36	20.931440556333563	33.590729806664065	26.719104255916925	18.75872538108544
37	20.11253427536595	34.135542057153565	27.684803843632462	18.06711982384803
38	20.77800452576397	31.307810754243175	29.682521360168483	18.23166335982437
39	21.24495534129444	31.745577143802993	28.19564019523074	18.813827319671827
40	22.606060342067607	31.600852882179858	27.66683582018038	18.126250955572157
41	19.85804172501737	30.847393765422552	30.368246473367034	18.92631803619304
42	21.05754341183363	31.792294004778405	26.733043086231266	20.417119497156698
43	20.82994844810726	31.734142947060757	27.651372430300402	19.784536174531578
44	21.115258881103955	31.933424661711125	25.917839307065897	21.033477150119023
45	20.925995700742025	32.19161971386195	26.685672842584868	20.196711742811157
46	19.121026072146517	32.89661961585455	28.077595726006155	19.90475858599278
47	20.349603287821598	32.21840840337233	27.90836961422109	19.523618694584982
48	20.530154699237066	30.51721772235157	29.44610573038382	19.506521848027546
49	19.611280869608777	32.31750477513835	27.283953574983283	20.787260780269587
50	19.089990395274736	30.63863800204291	28.79588107564211	21.475490527040243
51	19.310507046732106	30.65519036304119	28.47659474375421	21.557707846472496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5879.0
1	4608.5
2	3338.0
3	2440.5
4	1543.0
5	1717.5
6	1892.0
7	2249.5
8	2607.0
9	3164.0
10	3721.0
11	4419.0
12	5117.0
13	5499.5
14	5882.0
15	5911.0
16	5940.0
17	5776.5
18	5613.0
19	6059.0
20	6505.0
21	6628.5
22	6752.0
23	8751.0
24	10750.0
25	11166.5
26	12825.0
27	14067.0
28	18590.5
29	23114.0
30	25361.0
31	27608.0
32	31998.0
33	36388.0
34	37405.5
35	38423.0
36	42689.5
37	46956.0
38	48056.5
39	49157.0
40	49864.5
41	50572.0
42	51675.5
43	52779.0
44	57190.0
45	61601.0
46	65114.5
47	68628.0
48	67120.0
49	65612.0
50	62285.0
51	58958.0
52	54101.0
53	49244.0
54	44434.5
55	39625.0
56	35783.5
57	31942.0
58	30713.5
59	29485.0
60	27878.0
61	26271.0
62	24598.0
63	22925.0
64	18888.5
65	14852.0
66	12765.5
67	10679.0
68	9033.0
69	7387.0
70	6208.5
71	5030.0
72	4516.5
73	4003.0
74	3194.0
75	1958.5
76	1532.0
77	1221.5
78	911.0
79	677.5
80	444.0
81	412.5
82	381.0
83	234.0
84	87.0
85	69.5
86	52.0
87	44.5
88	37.0
89	24.5
90	12.0
91	14.0
92	16.0
93	9.5
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	918298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.09765456463667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37957612025217	19.613167512414183
2	9.4079349235323	5.0986594144891955
3	3.908929645743922	3.1776847577350913
4	2.275205261885999	2.46610905000922
5	1.5786356443337197	2.138866168678889
6	1.1732152684555879	1.9074829244740195
7	0.8878686521429215	1.6841410624176913
8	0.7225301105244327	1.5663097078031867
9	0.593600545692047	1.4476664282888625
>10	6.103637307260599	35.246511187064655
>50	0.705108287056419	13.130293747846524
>100	0.25728563844207486	9.915903956230997
>500	0.003640834506255775	0.7086938443352464
>1k	0.0028317601715322697	1.8985102382122403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4710	0.5129053967230681	No Hit
AATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	3055	0.3326806766430941	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	2314	0.25198791677647125	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGT	2219	0.24164269115254525	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1996	0.21735863521427684	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	1555	0.16933500889689404	TruSeq Adapter, Index 14 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1470	0.16007875439127603	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	945	0.10290777068010601	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	936	0.10192769667362882	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0889711183080003E-4	0.0	0.0	0.21376503052386045	0.0
2	1.0889711183080003E-4	0.0	0.0	0.2990314690873769	0.0
3	1.0889711183080003E-4	0.0	0.0	0.4817608227394593	0.0
4	1.0889711183080003E-4	0.0	0.0	1.2131138257951122	0.0
5	1.0889711183080003E-4	0.0	0.0	1.3489085242481198	0.0
6	1.0889711183080003E-4	0.0	0.0	1.9208361555834816	0.0
7	1.0889711183080003E-4	0.0	0.0	2.3707990216683474	0.0
8	2.1779422366160006E-4	0.0	0.0	2.751067736181501	0.0
9	2.1779422366160006E-4	0.0	0.0	3.5453632698753563	0.0
10	2.1779422366160006E-4	0.0	0.0	4.373308011124929	0.0
11	2.1779422366160006E-4	0.0	0.0	5.370587761271396	0.0
12	2.1779422366160006E-4	0.0	0.0	5.733977423450775	0.0
13	2.1779422366160006E-4	0.0	0.0	5.936634948567894	0.0
14	2.1779422366160006E-4	0.0	0.0	6.185682643324934	0.0
15	2.1779422366160006E-4	0.0	0.0	6.361551478931676	0.0
16	2.1779422366160006E-4	0.0	0.0	6.647624191711188	0.0
17	2.1779422366160006E-4	0.0	0.0	6.988145460406099	0.0
18	2.1779422366160006E-4	0.0	0.0	7.419486920367898	0.0
19	2.1779422366160006E-4	0.0	0.0	7.650893283008348	0.0
20	2.1779422366160006E-4	0.0	0.0	7.886764427233861	0.0
21	2.1779422366160006E-4	0.0	0.0	8.148661981186935	0.0
22	2.1779422366160006E-4	0.0	0.0	8.470997432206103	0.0
23	2.1779422366160006E-4	0.0	0.0	8.763930663030955	0.0
24	2.1779422366160006E-4	0.0	0.0	8.993703568993944	0.0
25	3.266913354924001E-4	0.0	0.0	9.198647933459508	0.0
26	3.266913354924001E-4	0.0	0.0	9.394444940531287	0.0
27	3.266913354924001E-4	0.0	0.0	9.601349453009806	0.0
28	3.266913354924001E-4	0.0	0.0	9.808471759711988	0.0
29	3.266913354924001E-4	0.0	0.0	10.043907315490179	0.0
30	3.266913354924001E-4	0.0	0.0	10.327039806250259	0.0
31	3.266913354924001E-4	0.0	0.0	10.565088892712387	0.0
32	3.266913354924001E-4	0.0	0.0	10.807276069424086	0.0
33	3.266913354924001E-4	0.0	0.0	11.02942617755892	0.0
34	3.266913354924001E-4	0.0	0.0	11.261812614205846	0.0
35	3.266913354924001E-4	0.0	0.0	11.506940012936976	0.0
36	3.266913354924001E-4	0.0	0.0	11.771995583133144	0.0
37	3.266913354924001E-4	0.0	0.0	12.046634099170422	0.0
38	3.266913354924001E-4	0.0	0.0	12.297315250604923	0.0
39	4.355884473232001E-4	0.0	0.0	12.564113174590384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	35	1.2115743E-7	45.000004	1
ACGACTA	35	1.2115743E-7	45.000004	11
GTCGCAC	20	7.032546E-4	45.0	15
TAGCGAA	20	7.032546E-4	45.0	32
TAGGGCC	75	0.0	45.0	5
TCGTAAG	20	7.032546E-4	45.0	1
CGTAACG	25	3.8901904E-5	45.0	1
CACGTTA	25	3.8901904E-5	45.0	43
TATACCG	25	3.8901904E-5	45.0	1
CAATACG	20	7.032546E-4	45.0	1
CTAGCGA	20	7.032546E-4	45.0	31
CCTAGCG	30	2.165023E-6	44.999996	1
GTATACG	30	2.165023E-6	44.999996	1
AATACGC	30	2.165023E-6	44.999996	34
TAGTCCG	30	2.165023E-6	44.999996	1
CCACACG	30	2.165023E-6	44.999996	1
TTACCGG	85	0.0	42.352943	2
AGTACGG	150	0.0	42.0	2
CGATAAG	55	6.002665E-11	40.909092	1
TCGATAG	45	1.927583E-8	40.0	1
>>END_MODULE