FastQCFastQC Report
Sat 18 Jun 2016
SRR3554311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933291
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54050.579133410693985No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23970.25683307778602815No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT13480.14443512259306046Illumina Single End Adapter 2 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11260.1206483294063695No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10850.11625527300702568No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9730.10425472869662303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.032603E-445.00000444
GTTTCGA207.032603E-445.00000419
TAGAACG207.032603E-445.0000041
TAGCGGA207.032603E-445.0000043
CAACCCG406.8121153E-945.0000041
TACGGCA207.032603E-445.0000044
ATCACGC207.032603E-445.00000410
CGAATCC207.032603E-445.00000437
CGATACG207.032603E-445.0000041
CGATAAG207.032603E-445.0000041
TCGAATC207.032603E-445.00000436
GCCGTAA207.032603E-445.00000428
TAAACCG253.89024E-545.01
TGCGATA253.89024E-545.016
ATGGTCG253.89024E-545.09
CATTACG302.1650612E-644.9999961
TATAGCG1050.042.8571431
TTACGCG850.042.352941
ATAGCCG556.002665E-1140.9090921
GTATGCG556.002665E-1140.9090921