##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933291
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25514442976521	34.0	31.0	34.0	30.0	34.0
2	32.435542612111334	34.0	31.0	34.0	30.0	34.0
3	32.53164875692576	34.0	31.0	34.0	30.0	34.0
4	36.047331432532836	37.0	35.0	37.0	35.0	37.0
5	35.881387477217714	37.0	35.0	37.0	35.0	37.0
6	35.860709039302854	37.0	35.0	37.0	35.0	37.0
7	35.9298386033938	37.0	35.0	37.0	35.0	37.0
8	36.07762852100792	37.0	35.0	37.0	35.0	37.0
9	37.69583763263548	39.0	38.0	39.0	35.0	39.0
10	37.25599732559298	39.0	37.0	39.0	34.0	39.0
11	37.21987354426433	39.0	37.0	39.0	34.0	39.0
12	37.23527495711413	39.0	37.0	39.0	34.0	39.0
13	37.2161062305326	39.0	37.0	39.0	34.0	39.0
14	38.48243688195858	40.0	38.0	41.0	34.0	41.0
15	38.471788541837434	40.0	38.0	41.0	34.0	41.0
16	38.46004943795665	40.0	38.0	41.0	34.0	41.0
17	38.41469273784918	40.0	38.0	41.0	34.0	41.0
18	38.33132538511568	40.0	38.0	41.0	34.0	41.0
19	38.31761904914973	40.0	38.0	41.0	34.0	41.0
20	38.25888709952201	40.0	38.0	41.0	34.0	41.0
21	38.2191170813819	40.0	37.0	41.0	34.0	41.0
22	38.156289946008265	40.0	37.0	41.0	33.0	41.0
23	38.076630975762114	40.0	37.0	41.0	33.0	41.0
24	38.038143515795184	40.0	37.0	41.0	33.0	41.0
25	37.973833456017466	40.0	37.0	41.0	33.0	41.0
26	37.902885595168065	40.0	37.0	41.0	33.0	41.0
27	37.8497917584119	40.0	37.0	41.0	33.0	41.0
28	37.735644080999386	40.0	37.0	41.0	33.0	41.0
29	37.662690414886676	40.0	36.0	41.0	33.0	41.0
30	37.599176462646696	40.0	36.0	41.0	33.0	41.0
31	37.49664252628602	40.0	36.0	41.0	33.0	41.0
32	37.472221418614346	40.0	36.0	41.0	33.0	41.0
33	37.361115664889084	40.0	36.0	41.0	32.0	41.0
34	37.2850590008904	39.0	36.0	41.0	32.0	41.0
35	37.20160378702891	39.0	35.0	41.0	32.0	41.0
36	37.159608310805524	39.0	35.0	41.0	32.0	41.0
37	37.07657633042641	39.0	35.0	41.0	31.0	41.0
38	37.01418207182969	39.0	35.0	41.0	31.0	41.0
39	36.92062282824971	39.0	35.0	41.0	31.0	41.0
40	36.83008515029074	39.0	35.0	41.0	31.0	41.0
41	36.754512793973156	39.0	35.0	41.0	31.0	41.0
42	36.683716011404805	39.0	35.0	41.0	31.0	41.0
43	36.60474385802499	39.0	35.0	41.0	31.0	41.0
44	36.54932277285434	39.0	35.0	41.0	30.0	41.0
45	36.4151288290576	39.0	35.0	40.0	30.0	41.0
46	36.2832128457255	38.0	35.0	40.0	30.0	41.0
47	36.18539340891533	38.0	35.0	40.0	30.0	41.0
48	36.08016149303915	38.0	35.0	40.0	30.0	41.0
49	35.993328983136024	38.0	35.0	40.0	29.0	41.0
50	35.90791403752956	38.0	35.0	40.0	29.0	41.0
51	34.92275721077349	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	7.0
12	7.0
13	14.0
14	22.0
15	35.0
16	60.0
17	112.0
18	225.0
19	435.0
20	750.0
21	1153.0
22	1734.0
23	2421.0
24	3315.0
25	4228.0
26	5272.0
27	6638.0
28	8639.0
29	11177.0
30	14251.0
31	18865.0
32	25006.0
33	33263.0
34	49124.0
35	65927.0
36	84754.0
37	118056.0
38	189417.0
39	288225.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.08397273733487	33.92618165181063	21.19103259326405	25.79881301759044
2	20.894126269298642	32.22510449581106	20.92444907322582	25.956320161664475
3	19.919724930380774	32.110563586276946	21.62937390374492	26.34033757959736
4	20.75697719146547	32.4746515288372	20.088696880179924	26.679674399517406
5	18.638666825245288	35.56607746137057	17.687409393211766	28.10784632017238
6	20.354530366198752	37.107075928086736	19.854686266127068	22.68370743958744
7	85.28454683480287	6.589263155864569	4.128615833646741	3.997574175685826
8	87.15202439539223	5.701651467763002	3.643879561680119	3.5024445751646596
9	77.6614153570537	11.71167406521653	6.415148115646674	4.211762462083102
10	31.493178440593557	43.404147259536416	13.098058376219207	12.004615923650823
11	21.831454498114734	29.01849476744124	32.27889264977375	16.871158084670267
12	25.909496609310494	25.052100577419047	31.06533760638429	17.97306520688617
13	22.836607231828015	27.37152720855553	29.32547297681002	20.466392582806435
14	19.393843935064197	28.83345066008351	28.8960249268449	22.876680478007394
15	18.45147976354642	32.25156998192418	29.64959482090795	19.647355433621453
16	21.103814351579516	30.759538021903136	28.710873671770116	19.425773954747232
17	20.052695247248714	32.13681477695596	28.39735945166084	19.413130524134488
18	19.647891172206737	32.031381423371705	28.066808744539486	20.25391865988207
19	19.99879994556896	32.93752966652416	27.879514535123555	19.18415585278332
20	21.972032302893737	31.537430447738164	27.917873417830023	18.572663831538073
21	21.734807257329173	32.74573525299183	27.02008269660802	18.49937479307097
22	20.26420484071956	31.39288817742805	27.896979613003875	20.445927368848515
23	20.639971884439042	31.89787536791847	28.08898832197032	19.373164425672165
24	19.09222311154827	32.3303235539612	28.79423459564059	19.783218738849943
25	19.409701797188657	34.345557816372384	25.542515678389698	20.702224708049258
26	19.470133109608902	34.079295739485325	26.134292519696427	20.316278631209343
27	19.00425483584434	33.17100454199173	26.53031048193972	21.294430140224218
28	19.31626898791481	33.669670017175775	27.71300698281672	19.301054012092692
29	19.588317041522956	32.15556562744096	27.524105557644933	20.73201177339115
30	19.384414935963168	33.003961251099604	26.905327491639795	20.70629632129743
31	20.422140575661825	33.595738092406336	25.420795871812757	20.561325460119086
32	21.359683099912033	31.824479181734315	25.951498514396903	20.86433920395675
33	19.353556393450702	33.865857487107455	25.841993547564478	20.93859257187737
34	18.930001467923724	32.08313377071031	27.338418563984867	21.648446197381098
35	18.6291306784272	32.10499190498998	27.645932511938938	21.61994490464389
36	18.75310058706234	34.82000790750152	26.38308951870317	20.04380198673297
37	18.829711204758215	35.70944110679306	26.118006066703742	19.342841621744984
38	18.74002856558137	33.39365749803652	28.4625052636316	19.403808672750515
39	19.406701661111057	34.38113085843536	26.519595710234	19.692571770219576
40	21.082384808168083	32.97792435585471	27.23619964191233	18.703491194064874
41	17.99353042084409	33.45483884447616	28.52786537103647	20.023765363643278
42	18.14214430440238	33.32883312921693	27.309381532662375	21.219641033718315
43	18.459837285476876	32.90131373815884	27.95923243661409	20.679616539750196
44	19.732859311833074	32.45697215552277	26.54681123036652	21.263357302277637
45	18.563770571022328	33.1750761552399	26.861396927646364	21.39975634609141
46	17.806129063711104	33.93561065091167	27.41942223808008	20.838838047297145
47	18.73542121374791	32.91663586169801	27.610573765310072	20.73736915924401
48	18.720313385642847	31.810657126233938	29.188109603542735	20.280919884580477
49	18.059747709985416	33.96014747811776	27.01118943609228	20.968915375804546
50	17.722018105821228	31.963556918474517	28.1843497901512	22.130075185553057
51	17.933099108423846	31.877731597111726	28.62965570224078	21.55951359222365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6573.0
1	5171.5
2	3770.0
3	2757.0
4	1744.0
5	1865.5
6	1987.0
7	2339.0
8	2691.0
9	3387.5
10	4084.0
11	4967.0
12	5850.0
13	6543.5
14	7237.0
15	7416.0
16	7595.0
17	7734.5
18	7874.0
19	8281.0
20	8688.0
21	8815.0
22	8942.0
23	10329.0
24	11716.0
25	11885.5
26	13709.5
27	15364.0
28	19447.0
29	23530.0
30	26141.0
31	28752.0
32	32603.0
33	36454.0
34	42253.0
35	48052.0
36	47888.0
37	47724.0
38	47212.0
39	46700.0
40	50341.0
41	53982.0
42	53817.0
43	53652.0
44	57171.5
45	60691.0
46	62675.5
47	64660.0
48	64538.0
49	64416.0
50	62022.5
51	59629.0
52	54666.0
53	49703.0
54	45330.0
55	40957.0
56	36807.0
57	32657.0
58	30953.5
59	29250.0
60	27214.5
61	25179.0
62	22339.5
63	19500.0
64	16835.5
65	14171.0
66	11751.5
67	9332.0
68	7469.0
69	5606.0
70	5008.0
71	4410.0
72	3703.0
73	2996.0
74	2753.0
75	1881.0
76	1252.0
77	984.0
78	716.0
79	498.0
80	280.0
81	216.5
82	153.0
83	117.0
84	81.0
85	69.0
86	57.0
87	52.0
88	47.0
89	29.0
90	11.0
91	6.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	933291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.125068233611554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29714796497495	19.339400034118448
2	9.323764572611555	5.05815500452437
3	4.295522159292529	3.4954899500940084
4	2.6810236692607172	2.908917998584983
5	1.8283230857050872	2.479669422641886
6	1.355747968207974	2.2064853687132917
7	1.0333473427985989	1.962073202770323
8	0.7972623838828905	1.7300637250332171
9	0.6644779590411132	1.6221608980849025
>10	5.74388603541102	33.10738075330253
>50	0.7061766575109493	13.337122581018676
>100	0.26814820331584477	10.990165306524071
>500	0.0031827679918794596	0.5368820934795485
>1k	0.0015913839959397298	0.6427476881938071
>5k	3.9784599898493245E-4	0.5832859729159717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5405	0.579133410693985	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2397	0.25683307778602815	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1348	0.14443512259306046	Illumina Single End Adapter 2 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1126	0.1206483294063695	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1085	0.11625527300702568	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	973	0.10425472869662303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2144315117149956E-4	0.0	0.0	0.17304356304732393	0.0
2	3.2144315117149956E-4	0.0	0.0	0.256725930068971	0.0
3	3.2144315117149956E-4	0.0	0.0	0.36494512429670917	0.0
4	3.2144315117149956E-4	0.0	0.0	0.8163584562585517	0.0
5	3.2144315117149956E-4	0.0	0.0	0.9100055609665153	0.0
6	3.2144315117149956E-4	0.0	0.0	1.4878531990558144	0.0
7	3.2144315117149956E-4	0.0	0.0	1.9835185381622666	0.0
8	3.2144315117149956E-4	0.0	0.0	2.523114441262157	0.0
9	3.2144315117149956E-4	0.0	0.0	3.1443569047596087	0.0
10	3.2144315117149956E-4	0.0	0.0	4.061755658203069	0.0
11	3.2144315117149956E-4	0.0	0.0	4.819182870080178	0.0
12	3.2144315117149956E-4	0.0	0.0	5.16398422357014	0.0
13	3.2144315117149956E-4	0.0	0.0	5.345278160830866	0.0
14	3.2144315117149956E-4	0.0	0.0	5.522607632560477	0.0
15	3.2144315117149956E-4	0.0	0.0	5.665864130265908	0.0
16	3.2144315117149956E-4	0.0	0.0	5.899446153450532	0.0
17	3.2144315117149956E-4	0.0	0.0	6.223032258963174	0.0
18	3.2144315117149956E-4	0.0	0.0	6.565690658111993	0.0
19	3.2144315117149956E-4	0.0	0.0	6.763485343799522	0.0
20	3.2144315117149956E-4	0.0	0.0	6.974673494119197	0.0
21	3.2144315117149956E-4	0.0	0.0	7.229256469847026	0.0
22	3.2144315117149956E-4	0.0	0.0	7.524126987188348	0.0
23	3.2144315117149956E-4	0.0	0.0	7.8018538698005235	0.0
24	3.2144315117149956E-4	0.0	0.0	8.012613429251969	0.0
25	3.2144315117149956E-4	0.0	0.0	8.204086399633127	0.0
26	3.2144315117149956E-4	0.0	0.0	8.397273733487198	0.0
27	3.2144315117149956E-4	0.0	0.0	8.585853715507811	0.0
28	3.2144315117149956E-4	0.0	0.0	8.78514846923414	0.0
29	3.2144315117149956E-4	0.0	0.0	9.004051255181931	0.0
30	4.2859086822866606E-4	0.0	0.0	9.284028239852308	0.0
31	5.357385852858326E-4	0.0	0.0	9.501216662327185	0.0
32	5.357385852858326E-4	0.0	0.0	9.735548719531208	0.0
33	5.357385852858326E-4	0.0	0.0	9.970523663037573	0.0
34	5.357385852858326E-4	0.0	0.0	10.204427129373368	0.0
35	5.357385852858326E-4	0.0	0.0	10.467581922465769	0.0
36	5.357385852858326E-4	0.0	0.0	10.721843455042425	0.0
37	5.357385852858326E-4	0.0	0.0	10.96024712549462	0.0
38	5.357385852858326E-4	0.0	0.0	11.217937385017105	0.0
39	5.357385852858326E-4	0.0	0.0	11.47648482627605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.032603E-4	45.000004	44
GTTTCGA	20	7.032603E-4	45.000004	19
TAGAACG	20	7.032603E-4	45.000004	1
TAGCGGA	20	7.032603E-4	45.000004	3
CAACCCG	40	6.8121153E-9	45.000004	1
TACGGCA	20	7.032603E-4	45.000004	4
ATCACGC	20	7.032603E-4	45.000004	10
CGAATCC	20	7.032603E-4	45.000004	37
CGATACG	20	7.032603E-4	45.000004	1
CGATAAG	20	7.032603E-4	45.000004	1
TCGAATC	20	7.032603E-4	45.000004	36
GCCGTAA	20	7.032603E-4	45.000004	28
TAAACCG	25	3.89024E-5	45.0	1
TGCGATA	25	3.89024E-5	45.0	16
ATGGTCG	25	3.89024E-5	45.0	9
CATTACG	30	2.1650612E-6	44.999996	1
TATAGCG	105	0.0	42.857143	1
TTACGCG	85	0.0	42.35294	1
ATAGCCG	55	6.002665E-11	40.909092	1
GTATGCG	55	6.002665E-11	40.909092	1
>>END_MODULE