FastQCFastQC Report
Sat 18 Jun 2016
SRR3554309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725578
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18790.25896595541761186No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15220.20976380209984316No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9500.13093009986521092No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9050.1247281477663325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG351.2111013E-745.0000041
CTACGTA453.8380676E-1045.00000417
CCGAACG207.031564E-445.0000041
TGTACCG207.031564E-445.0000041
ATCGACG351.2111013E-745.0000041
CTATGCG253.88938E-544.9999961
TCGCACG253.88938E-544.9999961
CTACGCG253.88938E-544.9999961
ACTACCG302.1643918E-644.9999961
TCGTAAG253.88938E-544.9999961
TATAGCG502.1827873E-1144.9999961
TGCGGGT800.042.1875044
AGTACGG800.042.1875042
CGTTGAT1400.041.78571725
TCGACGG650.041.538462
CCACGCG556.002665E-1140.909091
GGCCTAT1500.040.5000048
CGCATAG501.0804797E-940.4999961
ATGCGTG501.0804797E-940.4999961
CATGACG451.9266736E-840.0000041
Produced by FastQC (version 0.11.5)