##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725578
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.447472222145656	34.0	31.0	34.0	31.0	34.0
2	32.628023451648204	34.0	31.0	34.0	31.0	34.0
3	32.70121475568443	34.0	31.0	34.0	31.0	34.0
4	36.179022792863066	37.0	37.0	37.0	35.0	37.0
5	36.03464686084749	37.0	35.0	37.0	35.0	37.0
6	36.017718288040705	37.0	35.0	37.0	35.0	37.0
7	36.05906463536656	37.0	36.0	37.0	35.0	37.0
8	36.189120949091624	37.0	37.0	37.0	35.0	37.0
9	37.831173216387484	39.0	38.0	39.0	35.0	39.0
10	37.43527091504979	39.0	37.0	39.0	35.0	39.0
11	37.422416335666185	39.0	37.0	39.0	35.0	39.0
12	37.41016541295353	39.0	37.0	39.0	35.0	39.0
13	37.41196122263906	39.0	37.0	39.0	35.0	39.0
14	38.72546301017947	40.0	38.0	41.0	35.0	41.0
15	38.72654490626783	40.0	38.0	41.0	35.0	41.0
16	38.706752409802945	40.0	38.0	41.0	35.0	41.0
17	38.67922814638812	40.0	38.0	41.0	35.0	41.0
18	38.61183635667013	40.0	38.0	41.0	34.0	41.0
19	38.62825774761638	40.0	38.0	41.0	34.0	41.0
20	38.55631096863466	40.0	38.0	41.0	34.0	41.0
21	38.52579736430818	40.0	38.0	41.0	34.0	41.0
22	38.46967107602491	40.0	38.0	41.0	34.0	41.0
23	38.41488440939499	40.0	38.0	41.0	34.0	41.0
24	38.39432838371616	40.0	38.0	41.0	34.0	41.0
25	38.328052945375966	40.0	38.0	41.0	34.0	41.0
26	38.276892353406524	40.0	38.0	41.0	34.0	41.0
27	38.21612011389541	40.0	38.0	41.0	34.0	41.0
28	38.11287966283432	40.0	37.0	41.0	34.0	41.0
29	38.075152499111056	40.0	37.0	41.0	33.0	41.0
30	38.0349652277219	40.0	37.0	41.0	33.0	41.0
31	37.9713704109	40.0	37.0	41.0	33.0	41.0
32	37.910075829201	40.0	37.0	41.0	33.0	41.0
33	37.82175727488981	40.0	37.0	41.0	33.0	41.0
34	37.75088136630383	40.0	37.0	41.0	33.0	41.0
35	37.70670141597458	40.0	37.0	41.0	33.0	41.0
36	37.62405420230492	40.0	37.0	41.0	33.0	41.0
37	37.56850538467263	40.0	37.0	41.0	33.0	41.0
38	37.51993996510368	40.0	36.0	41.0	33.0	41.0
39	37.46663763234277	40.0	36.0	41.0	32.0	41.0
40	37.362165060131375	40.0	36.0	41.0	32.0	41.0
41	37.29739876346857	40.0	36.0	41.0	32.0	41.0
42	37.25387070721549	40.0	36.0	41.0	32.0	41.0
43	37.198295703563225	39.0	36.0	41.0	32.0	41.0
44	37.128719448494856	39.0	36.0	41.0	31.0	41.0
45	37.02036721069272	39.0	35.0	41.0	31.0	41.0
46	36.9067943074349	39.0	35.0	41.0	31.0	41.0
47	36.833383867757846	39.0	35.0	41.0	31.0	41.0
48	36.720451832883576	39.0	35.0	41.0	31.0	41.0
49	36.62192900005237	39.0	35.0	41.0	31.0	41.0
50	36.52908164249743	39.0	35.0	40.0	30.0	41.0
51	35.54348533169418	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	4.0
12	11.0
13	11.0
14	16.0
15	31.0
16	37.0
17	81.0
18	131.0
19	256.0
20	436.0
21	698.0
22	1025.0
23	1406.0
24	1903.0
25	2546.0
26	3290.0
27	4231.0
28	5593.0
29	7194.0
30	9196.0
31	12104.0
32	16242.0
33	21646.0
34	33765.0
35	44850.0
36	54411.0
37	82937.0
38	152343.0
39	269053.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.182913484146432	34.14133284085239	21.573421465369677	25.102332209631495
2	22.324822417438238	31.151578465719744	20.63651323496578	25.887085881876242
3	20.905677956057104	31.131732219003332	22.29215880305081	25.670431021888756
4	21.88158957410506	31.07412297506264	19.821301086857652	27.22298636397465
5	18.8892165969751	34.59669394606782	17.55758857076703	28.956500886190046
6	20.329447695492423	38.127672007695935	19.228807929678133	22.314072367133512
7	87.37213642089479	5.539721435875951	3.518711978588105	3.5694301646411555
8	89.0468013087497	4.633960787124196	3.245550443921949	3.0736874602041406
9	79.50819898067472	11.079718514067407	5.435804282930298	3.976278222327579
10	30.95628588518395	45.307327399673085	12.423750444473233	11.312636270669728
11	23.564110267951897	27.92284220304364	31.12787322658625	17.38517430241821
12	26.041859042032694	25.649620027068075	30.771054249164116	17.537466681735115
13	23.622959902312363	26.00671464680572	29.753107178001535	20.61721827288038
14	19.567021050803636	28.295234971291855	30.328786153935205	21.808957823969305
15	19.961603025450056	30.727227120998705	29.877973146925623	19.433196706625612
16	21.85898690423359	29.994845488700044	29.15138000325258	18.994787603813787
17	20.933793472238683	29.822431220351227	28.71407346970277	20.529701837707318
18	20.417377594138742	30.538825598350556	28.96587272491724	20.077924082593462
19	21.84685864235134	30.156647527901892	28.53311428957328	19.463379540173488
20	23.959794811860338	28.91005515602733	29.22511432265036	17.905035709461973
21	23.52042096094424	29.36775922092456	28.665973885647027	18.44584593248417
22	22.222834760701122	29.12960426032763	28.106419985170444	20.541140993800806
23	22.13531832552806	29.38526250795917	29.149174864728533	19.330244301784234
24	20.912017729313735	28.948782901355884	29.95570427989823	20.18349508943215
25	20.192453464686086	31.77149803329208	27.18329387054183	20.852754631480007
26	21.546270697292368	31.0479369550896	27.20548307694004	20.200309270677998
27	20.990713610390614	30.017034695098253	28.66211489322995	20.330136801281185
28	20.511371623726188	31.28595409452878	29.591994244588456	18.610680037156584
29	21.738393391199843	29.52391059265854	28.095945577181226	20.641750438960386
30	20.981755235136674	29.76137644746671	28.93086615084801	20.3260021665486
31	22.032503741844433	30.151410323907285	27.62459721766647	20.191488716581816
32	22.86783777898448	29.88830973375709	28.153416999964165	19.090435487294265
33	20.540038424538782	31.667305238030924	28.186494077824854	19.606162259605444
34	20.168472583237087	30.244715247705965	30.430773810672317	19.156038358384624
35	21.99556767156667	29.264531173767672	29.792524029118855	18.947377125546804
36	20.781776735237287	32.22961556166257	27.97397385256995	19.0146338505302
37	20.77474785619189	32.939532345247514	27.363150481409303	18.922569317151293
38	20.525705024132485	30.027233460771964	29.61983411845453	19.82722739664102
39	22.07536612190557	29.263566425663402	27.844835427755527	20.8162320246755
40	21.722543958058267	29.71341468456872	29.168607647971683	19.39543370940133
41	18.818927806521145	29.385538150274677	31.51074040282368	20.284793640380496
42	20.861023900945177	29.898508499430797	28.37544688510401	20.86502071452001
43	21.03302470582074	29.358938666828376	29.657872757994312	19.95016386935657
44	20.863366860626975	29.770334822720645	28.810823922445277	20.555474394207103
45	21.005736116585673	29.85330315968786	28.3120491525377	20.828911571188762
46	18.94379377544523	30.61517851974564	29.00404918561478	21.43697851919435
47	20.460515616515384	30.00862760447533	29.732709646654115	19.79814713235517
48	20.37864984881019	28.782294942790436	30.236032514767537	20.603022693631836
49	19.32238849579232	30.823288467952448	29.355493137884554	20.49882989837068
50	19.29868325665883	30.043358536229047	29.190107748581127	21.467850458530993
51	19.865541678496314	29.050081452304234	29.429365278440084	21.65501159075937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2379.0
1	1901.0
2	1423.0
3	1023.5
4	624.0
5	698.5
6	773.0
7	841.5
8	910.0
9	974.0
10	1038.0
11	1182.0
12	1326.0
13	1506.5
14	1687.0
15	1713.0
16	1739.0
17	1900.0
18	2061.0
19	2240.0
20	2419.0
21	3357.0
22	4295.0
23	5889.5
24	7484.0
25	8194.0
26	10798.0
27	12692.0
28	15858.5
29	19025.0
30	21547.5
31	24070.0
32	27480.0
33	30890.0
34	35004.5
35	39119.0
36	39338.5
37	39558.0
38	42620.5
39	45683.0
40	46711.5
41	47740.0
42	48119.5
43	48499.0
44	50855.5
45	53212.0
46	52359.0
47	51506.0
48	50003.0
49	48500.0
50	46143.0
51	43786.0
52	39414.5
53	35043.0
54	32522.5
55	30002.0
56	27510.0
57	25018.0
58	24025.5
59	23033.0
60	22710.0
61	22387.0
62	20161.5
63	17936.0
64	14728.5
65	11521.0
66	9403.0
67	7285.0
68	5868.0
69	4451.0
70	3742.5
71	3034.0
72	2385.5
73	1737.0
74	1464.5
75	1019.0
76	846.0
77	605.5
78	365.0
79	299.5
80	234.0
81	167.5
82	101.0
83	59.5
84	18.0
85	15.5
86	13.0
87	10.5
88	8.0
89	6.0
90	4.0
91	4.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	725578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.792360282498894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.07444532460212	16.910288100417027
2	8.28334851867258	3.941608246035231
3	3.5060410900761814	2.50250978341013
4	2.1218260985796493	2.0193300397686413
5	1.5877819317664625	1.8888539885314877
6	1.228710400863983	1.7540352324125714
7	1.0401851345080158	1.7323921636500001
8	0.8910889745804775	1.6960887941584977
9	0.7407354885803483	1.5861461056502844
>10	8.316390198509408	41.97624668070702
>50	0.9951587136719833	16.06528858117591
>100	0.208465078697817	6.687520307467874
>500	0.004658437512800379	0.7685037320390368
>1k	0.0011646093782000948	0.47118824457630504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1879	0.25896595541761186	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1522	0.20976380209984316	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	950	0.13093009986521092	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	905	0.1247281477663325	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09688827390025607	0.0
2	0.0	0.0	0.0	0.13947501164588783	0.0
3	0.0	0.0	0.0	0.21127983483512455	0.0
4	0.0	0.0	0.0	0.5013933719048813	0.0
5	0.0	0.0	0.0	0.5613455755273726	0.0
6	0.0	0.0	0.0	0.8889464675059057	0.0
7	0.0	0.0	0.0	1.1481880652390233	0.0
8	0.0	0.0	0.0	1.4210739575896734	0.0
9	0.0	0.0	0.0	1.800771247198785	0.0
10	0.0	0.0	0.0	2.250481684946346	0.0
11	0.0	0.0	0.0	2.6926119590175004	0.0
12	0.0	0.0	0.0	2.886801970291271	0.0
13	0.0	0.0	0.0	2.980244715247706	0.0
14	0.0	0.0	0.0	3.086504827875156	0.0
15	0.0	0.0	0.0	3.168508416738104	0.0
16	0.0	0.0	0.0	3.320111690266243	0.0
17	0.0	0.0	0.0	3.5034138300775384	0.0
18	0.0	0.0	0.0	3.7353668385755907	0.0
19	0.0	0.0	0.0	3.853617391927539	0.0
20	0.0	0.0	0.0	3.972557051068252	0.0
21	0.0	0.0	0.0	4.1132724531339155	0.0
22	0.0	0.0	0.0	4.2830681194854305	0.0
23	0.0	0.0	0.0	4.452863785836946	0.0
24	0.0	0.0	0.0	4.59164969169407	0.0
25	0.0	0.0	0.0	4.710175887361524	0.0
26	0.0	0.0	0.0	4.831596327341789	0.0
27	0.0	0.0	0.0	4.9549462635305925	0.0
28	0.0	0.0	0.0	5.086152005711309	0.0
29	0.0	0.0	0.0	5.215428251683486	0.0
30	0.0	0.0	0.0	5.389220731609834	0.0
31	0.0	0.0	0.0	5.53103870293752	0.0
32	0.0	0.0	0.0	5.677404772471051	0.0
33	0.0	0.0	0.0	5.829283688314695	0.0
34	0.0	0.0	0.0	5.972204228904404	0.0
35	0.0	0.0	0.0	6.1359357643147945	0.0
36	0.0	0.0	0.0	6.317859692548561	0.0
37	0.0	0.0	0.0	6.487241895426818	0.0
38	0.0	0.0	0.0	6.666547221663281	0.0
39	0.0	0.0	0.0	6.8341377494907505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	35	1.2111013E-7	45.000004	1
CTACGTA	45	3.8380676E-10	45.000004	17
CCGAACG	20	7.031564E-4	45.000004	1
TGTACCG	20	7.031564E-4	45.000004	1
ATCGACG	35	1.2111013E-7	45.000004	1
CTATGCG	25	3.88938E-5	44.999996	1
TCGCACG	25	3.88938E-5	44.999996	1
CTACGCG	25	3.88938E-5	44.999996	1
ACTACCG	30	2.1643918E-6	44.999996	1
TCGTAAG	25	3.88938E-5	44.999996	1
TATAGCG	50	2.1827873E-11	44.999996	1
TGCGGGT	80	0.0	42.187504	4
AGTACGG	80	0.0	42.187504	2
CGTTGAT	140	0.0	41.785717	25
TCGACGG	65	0.0	41.53846	2
CCACGCG	55	6.002665E-11	40.90909	1
GGCCTAT	150	0.0	40.500004	8
CGCATAG	50	1.0804797E-9	40.499996	1
ATGCGTG	50	1.0804797E-9	40.499996	1
CATGACG	45	1.9266736E-8	40.000004	1
>>END_MODULE