##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554308_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622568
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28394006759101	34.0	31.0	34.0	30.0	34.0
2	32.47152278947842	34.0	31.0	34.0	30.0	34.0
3	32.5701513730227	34.0	31.0	34.0	31.0	34.0
4	36.08566775035016	37.0	35.0	37.0	35.0	37.0
5	35.91426478713972	37.0	35.0	37.0	35.0	37.0
6	35.891178152426725	37.0	35.0	37.0	35.0	37.0
7	35.875216843782525	37.0	35.0	37.0	35.0	37.0
8	35.994358849153826	37.0	35.0	37.0	35.0	37.0
9	37.62839240050886	39.0	37.0	39.0	35.0	39.0
10	37.28962619344393	39.0	37.0	39.0	34.0	39.0
11	37.24090219863533	39.0	37.0	39.0	34.0	39.0
12	37.1148613484792	39.0	37.0	39.0	33.0	39.0
13	37.08459316893897	39.0	37.0	39.0	33.0	39.0
14	38.338862582079386	40.0	38.0	41.0	34.0	41.0
15	38.35422154688323	40.0	38.0	41.0	34.0	41.0
16	38.32683176777476	40.0	38.0	41.0	34.0	41.0
17	38.2989279885892	40.0	38.0	41.0	34.0	41.0
18	38.22376672106501	40.0	37.0	41.0	33.0	41.0
19	38.228882628082395	40.0	37.0	41.0	34.0	41.0
20	38.138953495843026	40.0	37.0	41.0	33.0	41.0
21	38.10942097891315	40.0	37.0	41.0	33.0	41.0
22	38.038662443299366	40.0	37.0	41.0	33.0	41.0
23	37.996683093252464	40.0	37.0	41.0	33.0	41.0
24	37.898407884761184	40.0	37.0	41.0	33.0	41.0
25	37.82480949872142	40.0	37.0	41.0	33.0	41.0
26	37.734828966474346	40.0	36.0	41.0	33.0	41.0
27	37.642140296321045	40.0	36.0	41.0	33.0	41.0
28	37.55363751429563	40.0	36.0	41.0	33.0	41.0
29	37.47370086480513	40.0	36.0	41.0	32.0	41.0
30	37.454469230670384	40.0	36.0	41.0	32.0	41.0
31	37.40060844759127	40.0	36.0	41.0	32.0	41.0
32	37.321399429459916	39.0	36.0	41.0	32.0	41.0
33	37.2091546626232	39.0	36.0	41.0	32.0	41.0
34	37.09933854615078	39.0	35.0	41.0	31.0	41.0
35	37.08002499325375	39.0	35.0	41.0	31.0	41.0
36	36.957166124824916	39.0	35.0	41.0	31.0	41.0
37	36.84836355225453	39.0	35.0	41.0	31.0	41.0
38	36.776832410274864	39.0	35.0	41.0	31.0	41.0
39	36.66953489418023	39.0	35.0	41.0	30.0	41.0
40	36.55373388931008	39.0	35.0	41.0	30.0	41.0
41	36.46182746302412	39.0	35.0	41.0	30.0	41.0
42	36.36475694221354	39.0	35.0	40.0	30.0	41.0
43	36.25864323254648	39.0	35.0	40.0	30.0	41.0
44	36.1447359967104	38.0	35.0	40.0	30.0	41.0
45	35.98330302874545	38.0	35.0	40.0	29.0	41.0
46	35.886918376787754	38.0	35.0	40.0	29.0	41.0
47	35.716067963660194	38.0	34.0	40.0	28.0	41.0
48	35.60267472790121	38.0	34.0	40.0	28.0	41.0
49	35.452435717865356	38.0	34.0	40.0	28.0	41.0
50	35.30513614577042	38.0	34.0	40.0	27.0	41.0
51	34.23896827334524	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	6.0
11	9.0
12	11.0
13	13.0
14	21.0
15	29.0
16	55.0
17	104.0
18	176.0
19	305.0
20	527.0
21	832.0
22	1292.0
23	1851.0
24	2440.0
25	3357.0
26	4144.0
27	5157.0
28	6648.0
29	8322.0
30	10441.0
31	13668.0
32	17875.0
33	23927.0
34	37301.0
35	46532.0
36	53122.0
37	77456.0
38	125298.0
39	181576.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.677901851685277	34.93674586551188	19.972276120841418	24.413076161961424
2	22.75638966345845	32.690886778633015	19.92826486423973	24.624458693668807
3	22.309852096477815	31.53647473047121	20.468125570218834	25.68554760283214
4	22.199663329949498	31.80648539597281	19.446229166934376	26.547622107143315
5	19.246411636961746	35.54455095668264	16.829486899423035	28.379550506932578
6	20.86968813045322	38.72556893383534	18.765500314825047	21.639242620886392
7	82.7091980313797	7.890543683581552	4.921068863160329	4.479189421878414
8	84.05330823299624	7.064770434715566	4.806703846005577	4.075217486282623
9	73.95545546831832	12.419044987856747	8.122646843397026	5.502852700427905
10	35.34666092699914	35.72284473342671	15.051849757777463	13.878644581796687
11	28.729070559360586	28.85291245293687	24.45258991788849	17.965427069814062
12	29.275838141375722	26.024145153621774	26.227657059148555	18.47235964585395
13	24.63457807018671	28.269201115380167	25.758792613818894	21.33742820061423
14	21.347065702059854	28.978199971729996	27.65786227367934	22.016872052530807
15	22.038556430783466	30.143855771578366	27.917271687590755	19.900316110047417
16	23.179636601945493	29.787910718186605	27.362794104419113	19.669658575448786
17	21.761638889245834	29.839150100872512	27.646297271944594	20.752913737937064
18	22.27387209108081	29.933758240063735	26.596452114467816	21.195917554387634
19	24.096805489520822	29.729925084488762	26.59050898857635	19.582760437414066
20	25.218771282815695	28.60474679071202	26.570270235540534	19.606211690931755
21	24.19639300445895	29.642223821333573	26.12790891918634	20.03347425502114
22	22.82401279860192	30.052299507844925	26.828715899307387	20.294971794245768
23	24.286824893023734	28.189531103429665	26.455905218385784	21.067738785160817
24	21.916320787448118	29.042128731319313	28.201899230284884	20.83965125094769
25	20.928637514295627	31.46403284460493	25.891308258696238	21.71602138240321
26	23.01274720191208	30.602922090438312	25.83476825021524	20.54956245743437
27	22.643630896544632	29.121959368293904	26.39856208478431	21.835847650377147
28	22.39691085953663	30.50959895143984	27.323119723467958	19.77037046555557
29	21.782680767402116	30.57031521054728	26.180915177137276	21.46608884491333
30	22.192595828889374	29.77570321635548	26.87288778093317	21.158813173821976
31	24.11077986661698	29.65828632374295	25.452962567944386	20.777971241695685
32	23.7888873183331	29.806061345909203	26.553083357962503	19.851967977795194
33	22.5467740070161	30.86538337980751	25.913313886997084	20.674528726179307
34	20.87306125595919	31.295376569306487	27.28392721758908	20.547634957145245
35	23.852976702946506	28.888410583261585	26.89135965870395	20.367253055087957
36	21.963705169555777	33.371133755670066	25.29346191901929	19.371699155754875
37	21.459985093997762	33.636325670448855	25.34357692653654	19.560112309016848
38	21.483275722491356	30.87261150589173	27.578031636704747	20.06608113491217
39	22.9994153249123	30.34560080184012	25.878779506816922	20.776204366430655
40	22.29780521967078	31.543381606507243	26.90212153531823	19.256691638503746
41	19.979825496973824	29.934240115136017	29.016589352488403	21.069345035401753
42	20.636299970444995	30.184815152722273	27.42768661415299	21.75119826267974
43	22.261985839297875	28.895799334369897	27.331954099793116	21.51026072653911
44	22.051727682759154	29.20516313077447	26.605768365865256	22.13734082060112
45	21.507208851081327	29.896011359401705	27.10659719098958	21.49018259852739
46	19.940954241143135	31.402352835352925	27.221604708240704	21.43508821526323
47	22.09043831356575	29.48834504825176	27.79439354415903	20.626823094023464
48	21.982016422302465	28.277232366584855	28.52009740301461	21.22065380809807
49	19.94529111679367	30.733028359954258	27.23236658485499	22.08931393839709
50	20.111698641754796	29.751127587669142	28.761034939155238	21.376138831420825
51	21.01296565194485	28.533589905038486	28.31819174772876	22.135252695287903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4041.0
1	3183.0
2	2325.0
3	1628.5
4	932.0
5	860.0
6	788.0
7	830.0
8	872.0
9	1062.5
10	1253.0
11	1334.5
12	1416.0
13	1463.0
14	1510.0
15	1655.5
16	1801.0
17	2052.5
18	2304.0
19	2073.0
20	1842.0
21	2218.0
22	2594.0
23	4825.5
24	7057.0
25	7627.0
26	9041.0
27	9885.0
28	11503.0
29	13121.0
30	15139.0
31	17157.0
32	20303.0
33	23449.0
34	25359.0
35	27269.0
36	27138.5
37	27008.0
38	29842.5
39	32677.0
40	33360.0
41	34043.0
42	34292.5
43	34542.0
44	38196.0
45	41850.0
46	41129.5
47	40409.0
48	40575.0
49	40741.0
50	38487.0
51	36233.0
52	34509.5
53	32786.0
54	30645.5
55	28505.0
56	28139.5
57	27774.0
58	26783.5
59	25793.0
60	25333.0
61	24873.0
62	22671.5
63	20470.0
64	17895.0
65	15320.0
66	12972.0
67	10624.0
68	9126.0
69	7628.0
70	6311.5
71	4995.0
72	4226.5
73	3458.0
74	2856.0
75	1729.5
76	1205.0
77	995.0
78	785.0
79	559.5
80	334.0
81	286.5
82	239.0
83	177.0
84	115.0
85	71.5
86	28.0
87	25.0
88	22.0
89	17.0
90	12.0
91	16.5
92	21.0
93	14.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	622568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.959235150986753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1614037399039	17.76850511331626
2	7.848895589876468	3.7610707022678644
3	3.109125765848849	2.2347682601389307
4	1.7600885007783764	1.6868149710678744
5	1.2420097393746852	1.4878801702746927
6	1.035008870253539	1.487881254345701
7	0.8731263618358026	1.4643607873824678
8	0.7114954508245043	1.3637509452129306
9	0.644383657360433	1.3895045616736343
>10	7.361900499438937	37.781878054094406
>50	0.8941094895736759	15.04104980737331
>100	0.35036694391734097	12.035164483313602
>500	0.004042695506738549	0.6034371289653698
>1k	0.004042695506738549	1.8939337605729583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3212	0.5159275773891366	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2633	0.42292568843885325	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2060	0.33088754963313244	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1441	0.23146065971909896	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1374	0.22069878310481747	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1012	0.16255252438287868	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	752	0.1207900181185027	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	729	0.11709564256434637	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15082689762403464	0.0
2	0.0	0.0	0.0	0.20174503026175453	0.0
3	0.0	0.0	0.0	0.2684044152606623	0.0
4	0.0	0.0	0.0	0.5673275850991377	0.0
5	0.0	0.0	0.0	0.623225093483764	0.0
6	0.0	0.0	0.0	0.9351588902738336	0.0
7	0.0	0.0	0.0	1.1667801750170264	0.0
8	0.0	0.0	0.0	1.422495213374282	0.0
9	0.0	0.0	0.0	1.7696058904408836	0.0
10	0.0	0.0	0.0	2.267382840107426	0.0
11	0.0	0.0	0.0	2.7025160303774047	0.0
12	0.0	0.0	0.0	2.9013698102054715	0.0
13	0.0	0.0	0.0	2.9983873247580988	0.0
14	0.0	0.0	0.0	3.1251204687680705	0.0
15	0.0	0.0	0.0	3.220049858007479	0.0
16	0.0	0.0	0.0	3.4007530101129517	0.0
17	0.0	0.0	0.0	3.6073167910975186	0.0
18	0.0	0.0	0.0	3.860140579021087	0.0
19	0.0	0.0	0.0	3.9841430976214647	0.0
20	0.0	0.0	0.0	4.148783747317562	0.0
21	0.0	0.0	0.0	4.3025018953752845	0.0
22	0.0	0.0	0.0	4.506335050950257	0.0
23	0.0	0.0	0.0	4.685592577838887	0.0
24	0.0	0.0	0.0	4.840274476041172	0.0
25	0.0	0.0	0.0	4.979536371930456	0.0
26	0.0	0.0	0.0	5.1069120160368024	0.0
27	0.0	0.0	0.0	5.238785160817774	0.0
28	0.0	0.0	0.0	5.372907055936058	0.0
29	0.0	0.0	0.0	5.516827077524062	0.0
30	0.0	0.0	0.0	5.694478354171753	0.0
31	0.0	0.0	0.0	5.843859626578944	0.0
32	0.0	0.0	0.0	5.999826524973979	0.0
33	0.0	0.0	0.0	6.163021549453233	0.0
34	0.0	0.0	0.0	6.315133447270017	0.0
35	0.0	0.0	0.0	6.476722221508333	0.0
36	0.0	0.0	0.0	6.6551766232764935	0.0
37	0.0	0.0	0.0	6.822708523406279	0.0
38	0.0	0.0	0.0	7.010157926523689	0.0
39	0.0	0.0	0.0	7.209493581424037	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	25	3.8887374E-5	45.000004	1
ATAGCGG	120	0.0	45.000004	2
GTTAGAC	25	3.8887374E-5	45.000004	21
ACGATAG	30	2.1638916E-6	45.000004	1
AATTCGC	30	2.1638916E-6	45.000004	24
CATAACG	25	3.8887374E-5	45.000004	1
GTATACG	25	3.8887374E-5	45.000004	1
TATACCG	25	3.8887374E-5	45.000004	1
GGCTAAT	30	2.1638916E-6	45.000004	10
TAATACG	75	0.0	45.0	1
TACTTCG	20	7.030793E-4	45.0	28
CGAAACG	20	7.030793E-4	45.0	1
CGTAGCG	20	7.030793E-4	45.0	1
TCTCGAT	20	7.030793E-4	45.0	22
CGAGCGT	20	7.030793E-4	45.0	39
TTACGTA	40	6.8066583E-9	45.0	12
AATAGGT	20	7.030793E-4	45.0	22
ATACGCA	20	7.030793E-4	45.0	37
CCACGCG	40	6.8066583E-9	45.0	1
GATTACG	20	7.030793E-4	45.0	1
>>END_MODULE