FastQCFastQC Report
Sat 18 Jun 2016
SRR3554307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608220
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30240.49718851731281444No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA26040.42813455657492355No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19700.32389595869915494No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13800.2268915852816415No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13300.21867087566998783No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA9600.1578376245437506No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA7630.12544802867383512No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT6750.11097957975732466No Hit
AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6220.10226562756897176No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.030663E-445.0000041
AACGTTG302.1638098E-645.0000041
CTTAACG207.030663E-445.0000041
CTATGCG302.1638098E-645.0000041
GATACGA253.888631E-545.00000419
CGAACGT207.030663E-445.00000438
GGTGTCG207.030663E-445.00000417
ATTAGCG502.1827873E-1145.0000041
CGAAACG253.888631E-545.0000041
TCTTACG253.888631E-545.0000041
ATTCGTT207.030663E-445.0000046
TAACGCG207.030663E-445.0000041
TCTAGCG302.1638098E-645.0000041
ATCGTGA207.030663E-445.00000442
CGTTAGC253.888631E-545.0000049
CGTTACT207.030663E-445.0000048
ACGATAG207.030663E-445.0000041
CATAGCG253.888631E-545.0000041
CTACGCG207.030663E-445.0000041
ACGTCAC207.030663E-445.00000411