FastQCFastQC Report
Sat 18 Jun 2016
SRR3554306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644598
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40970.6355899335710008No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA24330.3774445468338406No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10960.17002845184130264No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.12333268176444855No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7100.11014616861982197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG302.1640117E-645.0000041
TAGCACG600.045.0000041
GTTAACG502.1827873E-1145.0000041
TACGAAG302.1640117E-645.0000041
GATATCG253.8888917E-545.00000439
AATTCGC253.8888917E-545.00000432
CATACCG302.1640117E-645.0000041
ACGCTAG302.1640117E-645.0000041
CAGTACG253.8888917E-545.0000041
GGTTAAC302.1640117E-645.0000049
AGTACGG650.045.0000042
GATCGCA207.0309784E-445.09
ACATTCG207.0309784E-445.013
ATAGCCG207.0309784E-445.01
CGATTGT207.0309784E-445.017
CACGACT207.0309784E-445.033
CGTTAAT207.0309784E-445.012
ATCCGAT207.0309784E-445.021
CCCGTAG207.0309784E-445.01
TCGTAAT207.0309784E-445.045