##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.350793828091305	34.0	31.0	34.0	30.0	34.0
2	32.534274074694615	34.0	31.0	34.0	31.0	34.0
3	32.630659729009395	34.0	31.0	34.0	31.0	34.0
4	36.13127406538649	37.0	35.0	37.0	35.0	37.0
5	35.95844231598609	37.0	35.0	37.0	35.0	37.0
6	35.940746015346	37.0	35.0	37.0	35.0	37.0
7	35.971130844340195	37.0	35.0	37.0	35.0	37.0
8	36.0846077710449	37.0	35.0	37.0	35.0	37.0
9	37.71896282644377	39.0	38.0	39.0	35.0	39.0
10	37.349825782891045	39.0	37.0	39.0	34.0	39.0
11	37.30925786304022	39.0	37.0	39.0	34.0	39.0
12	37.24660951476734	39.0	37.0	39.0	34.0	39.0
13	37.23640470494789	39.0	37.0	39.0	34.0	39.0
14	38.52235656952085	40.0	38.0	41.0	34.0	41.0
15	38.522384493901626	40.0	38.0	41.0	34.0	41.0
16	38.530273131471084	40.0	38.0	41.0	34.0	41.0
17	38.489802946952985	40.0	38.0	41.0	34.0	41.0
18	38.410918122612856	40.0	38.0	41.0	34.0	41.0
19	38.41656660430222	40.0	38.0	41.0	34.0	41.0
20	38.32411208225902	40.0	38.0	41.0	34.0	41.0
21	38.27652893741526	40.0	37.0	41.0	34.0	41.0
22	38.21048467416902	40.0	37.0	41.0	34.0	41.0
23	38.14223748755038	40.0	37.0	41.0	34.0	41.0
24	38.10349706328596	40.0	37.0	41.0	33.0	41.0
25	38.04239386408273	40.0	37.0	41.0	33.0	41.0
26	37.93241058768411	40.0	37.0	41.0	33.0	41.0
27	37.850935001349676	40.0	37.0	41.0	33.0	41.0
28	37.75689654637464	40.0	37.0	41.0	33.0	41.0
29	37.675495425055615	40.0	37.0	41.0	33.0	41.0
30	37.63423560110332	40.0	36.0	41.0	33.0	41.0
31	37.587321090043716	40.0	36.0	41.0	33.0	41.0
32	37.50678717588326	40.0	36.0	41.0	33.0	41.0
33	37.42248036760896	40.0	36.0	41.0	32.0	41.0
34	37.33060450078964	40.0	36.0	41.0	32.0	41.0
35	37.3013133767092	40.0	36.0	41.0	32.0	41.0
36	37.1960322557625	39.0	35.0	41.0	32.0	41.0
37	37.115242368111595	39.0	35.0	41.0	31.0	41.0
38	37.0497302194546	39.0	35.0	41.0	31.0	41.0
39	36.98136978395837	39.0	35.0	41.0	31.0	41.0
40	36.90989733136001	39.0	35.0	41.0	31.0	41.0
41	36.825126668093915	39.0	35.0	41.0	31.0	41.0
42	36.727135361884464	39.0	35.0	41.0	31.0	41.0
43	36.64018349420879	39.0	35.0	41.0	31.0	41.0
44	36.549936549601455	39.0	35.0	41.0	30.0	41.0
45	36.409681693086235	39.0	35.0	40.0	30.0	41.0
46	36.31254828590843	39.0	35.0	40.0	30.0	41.0
47	36.179075951213	38.0	35.0	40.0	30.0	41.0
48	36.10418121061499	38.0	35.0	40.0	29.0	41.0
49	35.93407208834033	38.0	35.0	40.0	29.0	41.0
50	35.842334292070404	38.0	35.0	40.0	29.0	41.0
51	34.76534987697759	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	3.0
12	1.0
13	7.0
14	10.0
15	34.0
16	49.0
17	88.0
18	157.0
19	304.0
20	449.0
21	756.0
22	1220.0
23	1616.0
24	2204.0
25	2960.0
26	3695.0
27	4637.0
28	5848.0
29	7678.0
30	9836.0
31	12587.0
32	16607.0
33	22588.0
34	34709.0
35	45628.0
36	53164.0
37	78574.0
38	134070.0
39	205030.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.006735981185173	33.068827393197	20.06661516169768	25.85782146392015
2	22.130071765658595	31.75281338136327	20.64992444903645	25.467190403941682
3	22.214000043437927	31.20177226736664	20.67846937160835	25.905758317587086
4	22.089426278083394	31.334878482402985	19.421717101201057	27.153978138312564
5	19.404186795491142	35.45124247980912	16.72810030437575	28.416470420323986
6	21.472762869261153	37.706446498437785	18.73167462511519	22.089116007185876
7	85.46411872205623	6.53508077902817	4.400106733188747	3.6006937657268563
8	86.56294310562552	6.138709707445571	3.9399749921656597	3.3583721947632474
9	76.71432427652583	11.8593293804821	6.848609520972761	4.577736822019305
10	32.28927176317643	40.5258781442077	13.971653650802516	13.213196441813347
11	25.549567327233408	29.034995454531355	28.342470811265315	17.072966406969925
12	27.37675264273237	26.843707240791936	28.38978712313721	17.389752993338483
13	24.17537752211456	27.068188235148106	27.460060378716655	21.296373864020676
14	19.702977669803506	29.020567857796642	28.374428713709943	22.902025758689913
15	19.566613610343193	31.504596663346767	29.870865252451917	19.057924473858126
16	22.124952295849507	29.724572524270943	28.084015153630638	20.066460026248915
17	20.58926648857117	30.937421462679065	28.03809506079758	20.43521698795218
18	21.57065333742891	30.411822562279127	27.30833790982907	20.709186190462894
19	22.610991656815564	29.78988454819903	27.848054136066196	19.751069658919203
20	23.498831830070834	28.82509719235865	28.583396163190084	19.092674814380437
21	23.36277804150804	29.7247276597197	27.94175594711743	18.97073835165483
22	22.187471881699913	29.912596688168442	27.71960198449266	20.180329445638986
23	21.823058712561938	30.31998237661302	28.52909875612397	19.32786015470107
24	20.759915482207518	29.00489917747185	29.95681029106513	20.278375049255505
25	20.485170602453	32.107608152678104	26.516371443907676	20.89084980096122
26	21.673353004508236	32.293305284844195	26.390556594963062	19.642785115684504
27	21.667147586557824	30.676328502415455	26.912277109143997	20.744246801882724
28	21.181263361040525	31.12808292920549	28.674305536163626	19.016348173590362
29	21.66032162681237	30.000868758513054	26.955249628450602	21.383559986223972
30	21.06910043158682	32.01825013419216	26.639859261120886	20.272790173100134
31	22.144033956047025	30.93555983729394	25.830672760387095	21.089733446271943
32	22.60944030232796	30.323085085588225	27.40079863729022	19.666675974793595
33	21.28272194452977	31.470311729170742	26.88590408285474	20.361062243444753
34	20.652096345319098	30.720076698965865	28.826338275948732	19.801488679766305
35	22.055917021151167	29.397546998284202	28.875671348654507	19.67086463191012
36	21.865410690073503	32.792686294403644	26.570203444627506	18.771699570895347
37	21.563672242234695	32.3257285936351	27.331608227143118	18.778990936987082
38	21.404658407255376	29.572694919934595	29.68935677740235	19.33328989540768
39	22.42001991939162	29.479458515229645	28.116748733319064	19.98377283205967
40	22.330817036354443	29.90390910303786	28.678959599626435	19.08631426098126
41	19.0064195048697	29.711541146575076	30.483029733260107	20.799009615295112
42	20.520386349321594	30.36435111495847	28.198815385713267	20.91644715000667
43	20.913344441031466	30.38358791060475	28.0564010437513	20.646666604612488
44	21.75960831401897	29.96301570901554	27.077496362073727	21.199879614891763
45	20.589111353122412	30.619238657271662	27.76769397360836	21.023956015997566
46	19.3726942993928	31.187034399734408	28.41755636846531	21.022714932407485
47	19.934905165700172	30.178964253689895	29.494351518310637	20.391779062299292
48	20.392709874991855	29.370088023853626	30.004126602937024	20.23307549821749
49	19.267977871479587	31.767240978097977	28.532046329650417	20.432734820772016
50	19.390224605102716	30.203320519145265	29.07408958761895	21.33236528813307
51	19.82072547541258	29.152122718345385	28.89537355064707	22.131778255594963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4968.0
1	3889.5
2	2811.0
3	1901.5
4	992.0
5	938.5
6	885.0
7	1078.5
8	1272.0
9	1495.5
10	1719.0
11	1826.5
12	1934.0
13	1954.5
14	1975.0
15	2141.0
16	2307.0
17	2636.0
18	2965.0
19	2887.5
20	2810.0
21	3360.0
22	3910.0
23	5486.5
24	7063.0
25	7696.5
26	9070.0
27	9810.0
28	12957.0
29	16104.0
30	18273.5
31	20443.0
32	22053.5
33	23664.0
34	25833.5
35	28003.0
36	31417.0
37	34831.0
38	35247.0
39	35663.0
40	37204.0
41	38745.0
42	40082.5
43	41420.0
44	40851.5
45	40283.0
46	41769.0
47	43255.0
48	42841.0
49	42427.0
50	39614.0
51	36801.0
52	34890.0
53	32979.0
54	30536.0
55	28093.0
56	26871.0
57	25649.0
58	23831.0
59	22013.0
60	22419.0
61	22825.0
62	20343.0
63	17861.0
64	15157.0
65	12453.0
66	10755.5
67	9058.0
68	7506.0
69	5954.0
70	5146.0
71	4338.0
72	3794.0
73	3250.0
74	2587.5
75	1521.0
76	1117.0
77	978.0
78	839.0
79	631.0
80	423.0
81	320.0
82	217.0
83	168.0
84	119.0
85	90.5
86	62.0
87	38.0
88	14.0
89	11.0
90	8.0
91	5.5
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.994768312928507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14000885372258	17.789723351635413
2	8.011928620632197	3.8448874198358096
3	3.1304685124790885	2.2534460000356105
4	1.8637283791066397	1.7887892261957443
5	1.2956330736502597	1.5544207710402709
6	0.9736800550711964	1.4017936399411695
7	0.7678654590707352	1.2897327650131944
8	0.6635053432954505	1.2736525589411543
9	0.615444015036049	1.3290692895331633
>10	7.139964112157922	37.400694699714485
>50	1.075310462482199	17.891865560899475
>100	0.31726209533936706	10.484058527485056
>500	0.0032506362227394166	0.5082366819311428
>1k	0.0019503817336436501	1.1896295077983103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4097	0.6355899335710008	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2433	0.3774445468338406	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1096	0.17002845184130264	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	795	0.12333268176444855	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	710	0.11014616861982197	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6540634628093787E-4	0.0	0.0	0.12116078548180416	0.0
2	4.6540634628093787E-4	0.0	0.0	0.19159227921898608	0.0
3	4.6540634628093787E-4	0.0	0.0	0.2702459517404646	0.0
4	4.6540634628093787E-4	0.0	0.0	0.5930828206106752	0.0
5	4.6540634628093787E-4	0.0	0.0	0.6529651038321559	0.0
6	4.6540634628093787E-4	0.0	0.0	1.0687281065097938	0.0
7	4.6540634628093787E-4	0.0	0.0	1.35960707293538	0.0
8	4.6540634628093787E-4	0.0	0.0	1.6219411167890685	0.0
9	4.6540634628093787E-4	0.0	0.0	1.9528450289948154	0.0
10	4.6540634628093787E-4	0.0	0.0	2.5315002528707815	0.0
11	4.6540634628093787E-4	0.0	0.0	2.9875984722261006	0.0
12	4.6540634628093787E-4	0.0	0.0	3.2148719046599585	0.0
13	4.6540634628093787E-4	0.0	0.0	3.3338607938591185	0.0
14	4.6540634628093787E-4	0.0	0.0	3.459365371906211	0.0
15	4.6540634628093787E-4	0.0	0.0	3.5606688199466956	0.0
16	4.6540634628093787E-4	0.0	0.0	3.753967589102045	0.0
17	4.6540634628093787E-4	0.0	0.0	3.991635096602844	0.0
18	4.6540634628093787E-4	0.0	0.0	4.2651388927672755	0.0
19	4.6540634628093787E-4	0.0	0.0	4.409104589216845	0.0
20	4.6540634628093787E-4	0.0	0.0	4.587665490739965	0.0
21	4.6540634628093787E-4	0.0	0.0	4.769949643033333	0.0
22	4.6540634628093787E-4	0.0	0.0	4.9703846428316565	0.0
23	4.6540634628093787E-4	0.0	0.0	5.161976922050642	0.0
24	4.6540634628093787E-4	0.0	0.0	5.320990757029963	0.0
25	4.6540634628093787E-4	0.0	0.0	5.457975358285319	0.0
26	4.6540634628093787E-4	0.0	0.0	5.5912367087704276	0.0
27	4.6540634628093787E-4	0.0	0.0	5.755525149007599	0.0
28	4.6540634628093787E-4	0.0	0.0	5.9015076062910525	0.0
29	4.6540634628093787E-4	0.0	0.0	6.052144127037316	0.0
30	4.6540634628093787E-4	0.0	0.0	6.249011011514153	0.0
31	4.6540634628093787E-4	0.0	0.0	6.403836189376945	0.0
32	4.6540634628093787E-4	0.0	0.0	6.561608940766183	0.0
33	4.6540634628093787E-4	0.0	0.0	6.7251217037595525	0.0
34	4.6540634628093787E-4	0.0	0.0	6.891582040279368	0.0
35	4.6540634628093787E-4	0.0	0.0	7.066729961929761	0.0
36	4.6540634628093787E-4	0.0	0.0	7.24498059255536	0.0
37	4.6540634628093787E-4	0.0	0.0	7.420128514205753	0.0
38	4.6540634628093787E-4	0.0	0.0	7.609083490795814	0.0
39	4.6540634628093787E-4	0.0	0.0	7.800210363668519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACG	30	2.1640117E-6	45.000004	1
TAGCACG	60	0.0	45.000004	1
GTTAACG	50	2.1827873E-11	45.000004	1
TACGAAG	30	2.1640117E-6	45.000004	1
GATATCG	25	3.8888917E-5	45.000004	39
AATTCGC	25	3.8888917E-5	45.000004	32
CATACCG	30	2.1640117E-6	45.000004	1
ACGCTAG	30	2.1640117E-6	45.000004	1
CAGTACG	25	3.8888917E-5	45.000004	1
GGTTAAC	30	2.1640117E-6	45.000004	9
AGTACGG	65	0.0	45.000004	2
GATCGCA	20	7.0309784E-4	45.0	9
ACATTCG	20	7.0309784E-4	45.0	13
ATAGCCG	20	7.0309784E-4	45.0	1
CGATTGT	20	7.0309784E-4	45.0	17
CACGACT	20	7.0309784E-4	45.0	33
CGTTAAT	20	7.0309784E-4	45.0	12
ATCCGAT	20	7.0309784E-4	45.0	21
CCCGTAG	20	7.0309784E-4	45.0	1
TCGTAAT	20	7.0309784E-4	45.0	45
>>END_MODULE