##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	735244
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.380802835521266	34.0	31.0	34.0	30.0	34.0
2	32.54676270734613	34.0	31.0	34.0	31.0	34.0
3	32.63373247520551	34.0	31.0	34.0	31.0	34.0
4	36.12802280603446	37.0	35.0	37.0	35.0	37.0
5	35.97318169206413	37.0	35.0	37.0	35.0	37.0
6	35.95306048060236	37.0	35.0	37.0	35.0	37.0
7	35.95423288051314	37.0	35.0	37.0	35.0	37.0
8	36.076268558464946	37.0	35.0	37.0	35.0	37.0
9	37.699822099874325	39.0	38.0	39.0	35.0	39.0
10	37.35458568856053	39.0	37.0	39.0	35.0	39.0
11	37.30819700670798	39.0	37.0	39.0	34.0	39.0
12	37.248253640968166	39.0	37.0	39.0	34.0	39.0
13	37.233451752071424	39.0	37.0	39.0	34.0	39.0
14	38.51862510948746	40.0	38.0	41.0	34.0	41.0
15	38.509969479519725	40.0	38.0	41.0	34.0	41.0
16	38.50571510954187	40.0	38.0	41.0	34.0	41.0
17	38.47110619059795	40.0	38.0	41.0	34.0	41.0
18	38.40995098225895	40.0	38.0	41.0	34.0	41.0
19	38.41126891208905	40.0	38.0	41.0	34.0	41.0
20	38.32726278623151	40.0	38.0	41.0	34.0	41.0
21	38.261155480357544	40.0	37.0	41.0	34.0	41.0
22	38.213134687260286	40.0	37.0	41.0	34.0	41.0
23	38.13219149017197	40.0	37.0	41.0	34.0	41.0
24	38.10467545467899	40.0	37.0	41.0	33.0	41.0
25	38.04240497032278	40.0	37.0	41.0	33.0	41.0
26	37.961520257220734	40.0	37.0	41.0	33.0	41.0
27	37.88066138588006	40.0	37.0	41.0	33.0	41.0
28	37.79071437509181	40.0	37.0	41.0	33.0	41.0
29	37.709588925581166	40.0	36.0	41.0	33.0	41.0
30	37.66254739923073	40.0	36.0	41.0	33.0	41.0
31	37.58871748698391	40.0	36.0	41.0	33.0	41.0
32	37.54087486603087	40.0	36.0	41.0	33.0	41.0
33	37.40803325154643	40.0	36.0	41.0	32.0	41.0
34	37.30268319088629	40.0	36.0	41.0	32.0	41.0
35	37.21167530778898	39.0	35.0	41.0	32.0	41.0
36	37.144093661423966	39.0	35.0	41.0	31.0	41.0
37	37.07985240273977	39.0	35.0	41.0	31.0	41.0
38	36.99567626529424	39.0	35.0	41.0	31.0	41.0
39	36.909362062118156	39.0	35.0	41.0	31.0	41.0
40	36.8254538629353	39.0	35.0	41.0	31.0	41.0
41	36.73171355359581	39.0	35.0	41.0	31.0	41.0
42	36.66884190826447	39.0	35.0	41.0	31.0	41.0
43	36.59411569492577	39.0	35.0	41.0	31.0	41.0
44	36.52209606606786	39.0	35.0	41.0	31.0	41.0
45	36.37915168297871	39.0	35.0	40.0	30.0	41.0
46	36.2333062221521	38.0	35.0	40.0	30.0	41.0
47	36.14586994249528	38.0	35.0	40.0	30.0	41.0
48	36.00963217652915	38.0	35.0	40.0	29.0	41.0
49	35.900522819635384	38.0	35.0	40.0	29.0	41.0
50	35.80082122397462	38.0	35.0	40.0	29.0	41.0
51	34.810375875219655	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	4.0
12	6.0
13	10.0
14	18.0
15	32.0
16	65.0
17	100.0
18	201.0
19	334.0
20	589.0
21	918.0
22	1384.0
23	1926.0
24	2685.0
25	3359.0
26	4139.0
27	5149.0
28	6684.0
29	8422.0
30	10961.0
31	14441.0
32	18755.0
33	26010.0
34	40817.0
35	51342.0
36	63612.0
37	90970.0
38	149813.0
39	232376.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.342906572511982	33.60136226885225	19.21226150774437	26.843469650891404
2	22.44982618015244	31.5644874354636	19.854089254723604	26.131597129660356
3	21.394122223370747	31.55537481434735	20.595475787629685	26.455027174652223
4	22.13047641327233	30.793178863071308	19.372072400454815	27.70427232320155
5	19.650755395487757	33.935809064745854	17.0419615801013	29.37147395966509
6	21.634042576342	36.92162057765857	18.97329321966585	22.47104362633357
7	84.17056106544221	6.852555070153582	4.738971008263923	4.237912856140275
8	85.75384498207399	6.107088259135743	4.526660537182215	3.6124062216080643
9	75.91425431557414	12.000369945215466	7.304921903476941	4.780453835733443
10	34.5473067444277	37.45219274145726	14.529870355963462	13.47063015815158
11	26.30283280108372	28.92237134883114	26.900185516644814	17.874610333440327
12	27.941880518576145	25.65298050715137	27.81253570243348	18.59260327183901
13	24.046166986741817	27.452927191517375	27.00450462703538	21.49640119470543
14	20.229746859545948	28.729374194145073	28.3290989113818	22.71178003492718
15	20.503125493033604	31.02153842805926	28.253613766314313	20.221722312592828
16	21.733057325187286	29.819216477795123	28.461843959284266	19.985882237733325
17	21.462942914189032	30.594605328299178	27.863403169560037	20.079048587951757
18	20.85416541991502	31.108856379650835	26.684746832344093	21.35223136809005
19	22.240099885208174	31.545854165419918	26.280663290009848	19.93338265936206
20	23.994347454722515	29.72863430371414	27.74276294672245	18.534255294840897
21	22.965029296396843	31.468872918378114	26.16750357704381	19.39859420818123
22	21.87464297566522	30.364613652066524	27.593288758561783	20.16745461370647
23	22.358699968989885	30.590389040917028	27.252993564041322	19.797917426051757
24	20.546104422477434	29.63206772173591	29.286876193481348	20.534951662305303
25	20.273405835341737	32.648889348297985	25.833464808961377	21.244240007398904
26	21.9937870965285	32.015494176083045	25.336486934949487	20.654231792438974
27	20.281294373024465	32.11070066535735	26.307593125547434	21.300411836070747
28	21.236759497527352	31.293692978113384	27.46027169211854	20.009275832240725
29	21.123599784561314	31.216983749612375	25.85373019024977	21.805686275576544
30	20.2305629151683	31.80631191822035	26.549009580493006	21.41411558611835
31	21.21118975469368	31.91552736234502	25.271202485161385	21.602080397799913
32	22.89933681879757	30.314562240562314	25.6189781895534	21.167122751086715
33	20.738149512270756	32.41862565352454	26.32445827507603	20.51876655912867
34	19.750450190684997	31.363057706013244	27.61369014912056	21.272801954181197
35	21.49123284243054	30.474101114731976	27.55738231117833	20.47728373165915
36	20.79228120188672	33.332471941287515	25.777973026641497	20.097273830184264
37	20.280614326672506	34.11887754269331	26.188176986143375	19.412331144490807
38	20.59234757441067	32.1165490639842	27.64769246671853	19.643410894886596
39	21.12754405340268	32.33035563704022	26.05243973429229	20.48966057526481
40	22.221058587353316	31.676015037184936	27.53874904113464	18.56417733432711
41	19.016816186191253	31.121777260338064	29.365217533227067	20.49618902024362
42	19.82484726158935	31.654933600274195	26.45176295216282	22.068456185973638
43	20.087617171986444	31.525180756320353	27.43225378241781	20.954948289275396
44	21.083477049795714	31.402636403697276	26.47515654667022	21.03872999983679
45	20.7290368911545	31.629499866710916	26.23931647181072	21.402146770323867
46	18.567577566086904	33.51282023382714	26.828508631148303	21.09109356893766
47	20.526383078270616	31.561767250055762	27.627563094700534	20.284286576973088
48	20.221042266240865	30.066889359178724	28.227091958587895	21.484976415992513
49	19.6517074603805	32.6869719440077	26.458699424952805	21.202621170658993
50	18.890871601808378	31.686623760275502	27.96255392767571	21.45995071024041
51	19.02592880730751	30.510959627008177	28.194857761505027	22.26825380417929
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3740.0
1	3042.0
2	2344.0
3	1750.5
4	1157.0
5	1197.5
6	1238.0
7	1518.0
8	1798.0
9	2147.0
10	2496.0
11	3023.5
12	3551.0
13	3862.5
14	4174.0
15	4188.0
16	4202.0
17	4410.5
18	4619.0
19	4665.5
20	4712.0
21	4865.0
22	5018.0
23	6831.5
24	8645.0
25	8541.0
26	9340.0
27	10243.0
28	13807.5
29	17372.0
30	19893.5
31	22415.0
32	24483.5
33	26552.0
34	29529.0
35	32506.0
36	34548.0
37	36590.0
38	38430.0
39	40270.0
40	39437.5
41	38605.0
42	41377.0
43	44149.0
44	44043.5
45	43938.0
46	45970.5
47	48003.0
48	47651.5
49	47300.0
50	45885.0
51	44470.0
52	41896.0
53	39322.0
54	35627.5
55	31933.0
56	30143.0
57	28353.0
58	28102.5
59	27852.0
60	26609.5
61	25367.0
62	23500.0
63	21633.0
64	19064.5
65	16496.0
66	13765.5
67	11035.0
68	9633.0
69	8231.0
70	7239.0
71	6247.0
72	5076.0
73	3905.0
74	3230.5
75	2187.0
76	1818.0
77	1383.0
78	948.0
79	696.0
80	444.0
81	351.5
82	259.0
83	198.0
84	137.0
85	112.5
86	88.0
87	63.0
88	38.0
89	30.5
90	23.0
91	15.0
92	7.0
93	6.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	735244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.394747516986516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71928638969514	18.666177088374646
2	9.053725944261437	4.779416207735419
3	4.149063106588994	3.285404193913806
4	2.489591915350526	2.62848600104032
5	1.7522369745727673	2.312492626688826
6	1.3285003235034458	2.1039258369065017
7	0.989714624161963	1.828628733303695
8	0.8250090414939203	1.7420724279570463
9	0.7059328814557336	1.6769628152966576
>10	7.038572111491854	38.42173532390595
>50	0.747810453526532	13.57973695976748
>100	0.19433743594738817	7.186480457624119
>500	0.0041458653002109476	0.6743566305547777
>1k	0.0020729326501054738	1.1141246969307634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2859	0.38885050405035604	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2673	0.3635527797574683	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1502	0.2042859241285886	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1111	0.15110629940536746	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	794	0.10799136069114472	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	737	0.10023883227880813	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.440370815674796E-4	0.0	0.0	0.0931663502184309	0.0
2	5.440370815674796E-4	0.0	0.0	0.15069827159419186	0.0
3	5.440370815674796E-4	0.0	0.0	0.22577538885050405	0.0
4	5.440370815674796E-4	0.0	0.0	0.5037783375314862	0.0
5	5.440370815674796E-4	0.0	0.0	0.5724630190793805	0.0
6	5.440370815674796E-4	0.0	0.0	1.0312222881111577	0.0
7	5.440370815674796E-4	0.0	0.0	1.3641729820304551	0.0
8	5.440370815674796E-4	0.0	0.0	1.6904592217005512	0.0
9	5.440370815674796E-4	0.0	0.0	2.0934546898716615	0.0
10	5.440370815674796E-4	0.0	0.0	2.704544341742333	0.0
11	5.440370815674796E-4	0.0	0.0	3.202474280646969	0.0
12	5.440370815674796E-4	0.0	0.0	3.4389944018584306	0.0
13	5.440370815674796E-4	0.0	0.0	3.5594986154256274	0.0
14	5.440370815674796E-4	0.0	0.0	3.6877553574051607	0.0
15	5.440370815674796E-4	0.0	0.0	3.782825837409078	0.0
16	5.440370815674796E-4	0.0	0.0	3.9483491194759837	0.0
17	5.440370815674796E-4	0.0	0.0	4.158755460772206	0.0
18	5.440370815674796E-4	0.0	0.0	4.375962265588022	0.0
19	5.440370815674796E-4	0.0	0.0	4.5059871280826505	0.0
20	5.440370815674796E-4	0.0	0.0	4.649612917616465	0.0
21	5.440370815674796E-4	0.0	0.0	4.812824042086708	0.0
22	5.440370815674796E-4	0.0	0.0	5.002692983553759	0.0
23	5.440370815674796E-4	0.0	0.0	5.184129350256513	0.0
24	5.440370815674796E-4	0.0	0.0	5.326939084167977	0.0
25	5.440370815674796E-4	0.0	0.0	5.458188030096132	0.0
26	5.440370815674796E-4	0.0	0.0	5.57733215095941	0.0
27	5.440370815674796E-4	0.0	0.0	5.715245551136766	0.0
28	5.440370815674796E-4	0.0	0.0	5.8509828029878514	0.0
29	5.440370815674796E-4	0.0	0.0	5.998824879903815	0.0
30	5.440370815674796E-4	0.0	0.0	6.1729167460054075	0.0
31	5.440370815674796E-4	0.0	0.0	6.322118915625289	0.0
32	5.440370815674796E-4	0.0	0.0	6.481249761983777	0.0
33	5.440370815674796E-4	0.0	0.0	6.646909053321075	0.0
34	5.440370815674796E-4	0.0	0.0	6.801007556675063	0.0
35	5.440370815674796E-4	0.0	0.0	6.964626708956483	0.0
36	5.440370815674796E-4	0.0	0.0	7.132598157890442	0.0
37	5.440370815674796E-4	0.0	0.0	7.3011136439059685	0.0
38	5.440370815674796E-4	0.0	0.0	7.478333723226575	0.0
39	5.440370815674796E-4	0.0	0.0	7.6660265163673555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.031625E-4	45.000004	33
TCATACG	45	3.8380676E-10	45.000004	1
CGTTTCA	20	7.031625E-4	45.000004	23
CAATACG	20	7.031625E-4	45.000004	1
TCAACCG	45	3.8380676E-10	45.000004	1
CCCGATG	20	7.031625E-4	45.000004	1
TAATACG	25	3.889429E-5	45.0	1
CAAACGA	25	3.889429E-5	45.0	39
TAGCGCG	25	3.889429E-5	45.0	1
AACGACG	25	3.889429E-5	45.0	1
GCGATCG	35	1.2111377E-7	45.0	9
GTATCGC	25	3.889429E-5	45.0	40
GCGATAG	35	1.2111377E-7	45.0	1
TGTAGCG	25	3.889429E-5	45.0	1
CAGACGA	35	1.2111377E-7	45.0	30
CCAAACG	30	2.1644319E-6	44.999996	1
AATACGG	85	0.0	42.35294	2
CCGGGTC	70	0.0	41.785713	5
TTTACGG	70	0.0	41.785713	2
TCTACGG	60	3.6379788E-12	41.249996	2
>>END_MODULE