##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287441
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20552475802775	33.0	31.0	34.0	30.0	34.0
2	32.395197139131035	34.0	31.0	34.0	30.0	34.0
3	32.49846478401729	34.0	31.0	34.0	30.0	34.0
4	36.02827391701833	37.0	35.0	37.0	35.0	37.0
5	35.85562445191663	37.0	35.0	37.0	35.0	37.0
6	35.834794759526844	37.0	35.0	37.0	35.0	37.0
7	35.97752130000521	37.0	35.0	37.0	35.0	37.0
8	36.110630312379364	37.0	35.0	37.0	35.0	37.0
9	37.745944085981414	39.0	38.0	39.0	35.0	39.0
10	37.22987305826054	39.0	37.0	39.0	34.0	39.0
11	37.17219507534714	39.0	37.0	39.0	34.0	39.0
12	37.19768206853751	39.0	37.0	39.0	34.0	39.0
13	37.188623012627374	39.0	37.0	39.0	33.0	39.0
14	38.42971056537736	40.0	38.0	41.0	34.0	41.0
15	38.41798187256737	40.0	38.0	41.0	34.0	41.0
16	38.41759738892889	40.0	38.0	41.0	34.0	41.0
17	38.39525927790089	40.0	38.0	41.0	34.0	41.0
18	38.31937075174707	40.0	38.0	41.0	34.0	41.0
19	38.308098002160875	40.0	38.0	41.0	34.0	41.0
20	38.243486109266364	40.0	37.0	41.0	34.0	41.0
21	38.21078791183441	40.0	37.0	41.0	34.0	41.0
22	38.15011251001017	40.0	37.0	41.0	33.0	41.0
23	38.07565783597074	40.0	37.0	41.0	33.0	41.0
24	38.035729792666224	40.0	37.0	41.0	33.0	41.0
25	37.97100371978211	40.0	37.0	41.0	33.0	41.0
26	37.850093324664975	40.0	37.0	41.0	33.0	41.0
27	37.79184444180355	40.0	37.0	41.0	33.0	41.0
28	37.78141677948737	40.0	37.0	41.0	33.0	41.0
29	37.64497091517204	40.0	37.0	41.0	33.0	41.0
30	37.61950955422423	40.0	37.0	41.0	33.0	41.0
31	37.55176742079831	40.0	36.0	41.0	33.0	41.0
32	37.48632985899936	40.0	36.0	41.0	32.0	41.0
33	37.41138739561658	40.0	36.0	41.0	32.0	41.0
34	37.29801831695588	39.0	36.0	41.0	32.0	41.0
35	37.246088946988635	39.0	36.0	41.0	31.0	41.0
36	37.1334321339774	39.0	36.0	41.0	31.0	41.0
37	37.08466252045725	39.0	35.0	41.0	31.0	41.0
38	37.04553606728386	39.0	35.0	41.0	31.0	41.0
39	36.97052835819272	39.0	35.0	41.0	31.0	41.0
40	36.885588543475	39.0	35.0	41.0	31.0	41.0
41	36.81021499237635	39.0	35.0	41.0	31.0	41.0
42	36.70450995424256	39.0	35.0	41.0	30.0	41.0
43	36.63560504908575	39.0	35.0	41.0	30.0	41.0
44	36.53712985682451	39.0	35.0	41.0	30.0	41.0
45	36.3780553827321	39.0	35.0	40.0	30.0	41.0
46	36.25562880163052	39.0	35.0	40.0	30.0	41.0
47	36.12476688252122	39.0	35.0	40.0	29.0	41.0
48	36.0348140225455	38.0	35.0	40.0	29.0	41.0
49	35.91553166319855	38.0	35.0	40.0	28.0	41.0
50	35.79220950707644	38.0	35.0	40.0	28.0	41.0
51	34.72978645235005	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	10.0
12	9.0
13	17.0
14	28.0
15	39.0
16	67.0
17	143.0
18	292.0
19	522.0
20	884.0
21	1432.0
22	2168.0
23	3244.0
24	4367.0
25	5871.0
26	7479.0
27	9630.0
28	12450.0
29	16177.0
30	20981.0
31	26942.0
32	34962.0
33	46364.0
34	68058.0
35	89864.0
36	110990.0
37	160543.0
38	269175.0
39	394585.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.893105781158127	34.42542221352279	20.540281069190744	25.141190936128332
2	20.79971043333248	32.07680973341691	21.348162750759066	25.775317082491544
3	20.733687990362277	31.58311720692443	22.49959415615939	25.1836006465539
4	21.0530035939511	31.41138118173959	20.534766253366175	27.000848970943135
5	18.230816014093072	33.965206949289325	17.970765262252794	29.833211774364806
6	21.461099964969268	35.26080030075164	20.195333223037018	23.08276651124207
7	86.48108923049678	5.686085808980761	4.410609884258774	3.422215076263689
8	88.2487818859272	4.906632614620786	3.576319225502372	3.268266273949641
9	79.02303872565811	10.48459696405505	6.547795199935376	3.9445691103514644
10	31.528202069065692	43.15669611267623	13.240296060168971	12.07480575808911
11	21.559589915188347	27.465879989840314	33.744458969381895	17.230071125589443
12	25.543617144397295	23.71098947446912	32.093198833965985	18.652194547167596
13	23.128360833622665	26.05369877143885	30.66734708619657	20.150593308741914
14	18.46671031915249	28.445187002744206	31.638809079406354	21.44929359869695
15	18.085644313020946	31.37503000137482	31.405400325141112	19.13392536046312
16	20.242714035050927	29.67778717626672	30.912562206734133	19.16693658194822
17	19.8764836602221	29.4544759721028	30.179402395915616	20.489637971759482
18	19.42155019142625	29.841755855219777	29.978616495823886	20.758077457530092
19	20.591933921632137	31.300385804087334	29.666912891542214	18.440767382738315
20	22.984509581409945	29.657592076064066	29.61526003910082	17.742638303425167
21	22.015067098220424	30.175829416648998	28.528142260499706	19.28096122463088
22	20.971679478904274	29.197454485292916	28.891654064147403	20.9392119716554
23	20.290094847064836	30.139866603595816	29.097411065827483	20.472627483511864
24	19.227055841782263	29.66745660577844	30.147090235591378	20.95839731684792
25	20.1922262845443	32.17149368398241	27.45034529737673	20.185934734096552
26	21.37216385061529	31.539853088413373	27.424091667113288	19.663891393858048
27	19.191869763352262	31.04740333731798	28.446585125065926	21.31414177426383
28	20.90845328057752	30.47992102162351	29.249495705045902	19.362129992753065
29	20.508279602715778	29.88517532065547	28.69941224491064	20.907132831718116
30	20.83971226642619	29.581006042218633	29.06354543625688	20.51573625509829
31	19.67569776013037	31.252538951299513	28.1963212294777	20.875442059092418
32	21.35530870929231	29.812628306850563	28.791455297757334	20.04060768609979
33	20.424935977648683	30.698649491510675	28.572105440171626	20.30430909066901
34	20.726852725678302	29.511022252670223	29.888748299922092	19.873376721729382
35	21.019914699003685	28.66500290110382	29.838571243264738	20.476511156627762
36	19.544041241501553	30.78043964733141	30.813373195354192	18.862145915812842
37	19.56066336243758	31.308386170706076	29.81286132723752	19.318089139618824
38	20.06414274518211	29.230155012928748	31.273899153436936	19.43180308845221
39	19.925573288407	29.474748745767766	29.738217129950034	20.8614608358752
40	21.160736686186006	28.886216921785152	30.249386185464033	19.703660206564805
41	17.817204827250336	28.975463729988405	31.65341169032212	21.55391975243914
42	19.68874690179977	30.052095591176602	28.78570746154581	21.47345004547781
43	19.040095817983115	29.794685737055136	30.232220350291783	20.93299809466997
44	21.3027237753031	29.629707303091944	28.630360536910043	20.43720838469491
45	20.984650947111362	29.792899247421822	28.762327749388128	20.460122056078685
46	18.30079980364149	31.35405816654899	29.803773532146327	20.541368497663193
47	19.610762745632616	30.197888679947276	30.213734066260123	19.977614508159984
48	19.763546446011894	27.871180116215033	31.26667552144137	21.0985979163317
49	18.46756472723799	29.769519535264138	29.99904461641349	21.76387112108438
50	18.236563850304595	28.783454931138593	30.332574463606488	22.647406754950325
51	18.687846666371506	28.127657888788686	30.851044824578366	22.333450620261434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8193.0
1	6262.5
2	4332.0
3	2916.0
4	1500.0
5	1552.0
6	1604.0
7	1813.5
8	2023.0
9	2635.5
10	3248.0
11	3828.0
12	4408.0
13	4979.0
14	5550.0
15	6087.0
16	6624.0
17	6188.5
18	5753.0
19	6216.5
20	6680.0
21	7277.5
22	7875.0
23	10593.0
24	13311.0
25	14989.0
26	18632.0
27	20597.0
28	25052.5
29	29508.0
30	36735.5
31	43963.0
32	47109.5
33	50256.0
34	55078.5
35	59901.0
36	65363.5
37	70826.0
38	70639.5
39	70453.0
40	73407.0
41	76361.0
42	81602.5
43	86844.0
44	89068.0
45	91292.0
46	97053.0
47	102814.0
48	98853.0
49	94892.0
50	92312.0
51	89732.0
52	81292.5
53	72853.0
54	63299.0
55	53745.0
56	48191.5
57	42638.0
58	39700.5
59	36763.0
60	35278.0
61	33793.0
62	31472.5
63	29152.0
64	23658.0
65	18164.0
66	14271.0
67	10378.0
68	8130.0
69	5882.0
70	4657.5
71	3433.0
72	2975.0
73	2517.0
74	1986.0
75	1127.0
76	799.0
77	517.0
78	235.0
79	205.5
80	176.0
81	129.5
82	83.0
83	68.5
84	54.0
85	52.5
86	51.0
87	38.0
88	25.0
89	24.5
90	24.0
91	15.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1287441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.409035011889614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99360009167715	18.06112375293506
2	9.489623508707092	4.632651049473643
3	3.6734458063520545	2.6899580190457915
4	1.9893501817530401	1.942324729492757
5	1.2966605207583808	1.5825116024863166
6	0.942875175161392	1.380880389741358
7	0.7609024343320777	1.3001025912170605
8	0.5920209218308894	1.1560527526993076
9	0.48811166305456083	1.0722901205889397
>10	5.279472575941462	29.09526887974545
>50	0.9869178242787663	16.625168268355598
>100	0.5025521657904904	18.808412934665302
>500	0.0025526459214644604	0.4454995092403801
>1k	0.0015954037009152876	0.6756482941713807
>5k	3.1908074018305755E-4	0.5321071061416568
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6832	0.5306650945557894	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2899	0.2251753672595482	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1691	0.1313458247795433	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1447	0.11239349997397938	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1436	0.11153909188848264	Illumina PCR Primer Index 5 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3302038695365457E-4	0.0	0.0	0.1487446803387495	0.0
2	2.3302038695365457E-4	0.0	0.0	0.25166201790994697	0.0
3	2.3302038695365457E-4	0.0	0.0	0.38471665886048373	0.0
4	2.3302038695365457E-4	0.0	0.0	1.0026090515992578	0.0
5	3.1069384927153945E-4	0.0	0.0	1.111817939618204	0.0
6	3.1069384927153945E-4	0.0	0.0	1.8191124874848634	0.0
7	3.1069384927153945E-4	0.0	0.0	2.2877941591109807	0.0
8	3.1069384927153945E-4	0.0	0.0	2.673442899519279	0.0
9	3.1069384927153945E-4	0.0	0.0	3.3032193319926892	0.0
10	3.1069384927153945E-4	0.0	0.0	4.338373564303141	0.0
11	3.1069384927153945E-4	0.0	0.0	5.278455478736501	0.0
12	3.1069384927153945E-4	0.0	0.0	5.698202869102351	0.0
13	3.1069384927153945E-4	0.0	0.0	5.8917651371985205	0.0
14	3.1069384927153945E-4	0.0	0.0	6.099541648898862	0.0
15	3.1069384927153945E-4	0.0	0.0	6.276714816445957	0.0
16	3.1069384927153945E-4	0.0	0.0	6.591214665371074	0.0
17	3.8836731158942427E-4	0.0	0.0	7.011505769973148	0.0
18	3.8836731158942427E-4	0.0	0.0	7.485469237036882	0.0
19	3.8836731158942427E-4	0.0	0.0	7.7546077839683525	0.0
20	3.8836731158942427E-4	0.0	0.0	8.0297271874983	0.0
21	3.8836731158942427E-4	0.0	0.0	8.334828547482953	0.0
22	3.8836731158942427E-4	0.0	0.0	8.688398147953965	0.0
23	3.8836731158942427E-4	0.0	0.0	9.046705829626367	0.0
24	3.8836731158942427E-4	0.0	0.0	9.311960703441944	0.0
25	4.6604077390730914E-4	0.0	0.0	9.554612599723017	0.0
26	4.6604077390730914E-4	0.0	0.0	9.778933558897068	0.0
27	4.6604077390730914E-4	0.0	0.0	10.034168556073638	0.0
28	4.6604077390730914E-4	0.0	0.0	10.278606941988022	0.0
29	4.6604077390730914E-4	0.0	0.0	10.550619407025254	0.0
30	4.6604077390730914E-4	0.0	0.0	10.89556725317898	0.0
31	4.6604077390730914E-4	0.0	0.0	11.180318166036347	0.0
32	4.6604077390730914E-4	0.0	0.0	11.457612426511195	0.0
33	4.6604077390730914E-4	0.0	0.0	11.726362606131078	0.0
34	4.6604077390730914E-4	0.0	0.0	12.003579193143608	0.0
35	5.43714236225194E-4	0.0	0.0	12.286310595980709	0.0
36	5.43714236225194E-4	0.0	0.0	12.593431465985626	0.0
37	5.43714236225194E-4	0.0	0.0	12.90653319258902	0.0
38	5.43714236225194E-4	0.0	0.0	13.198352390517313	0.0
39	5.43714236225194E-4	0.0	0.0	13.489472527284745	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAG	45	3.8562575E-10	45.000004	1
CTCCGCG	35	1.2120836E-7	45.0	1
CGCGCGA	20	7.033603E-4	45.0	32
ATGTCGA	20	7.033603E-4	45.0	3
CGGATTA	40	6.8157533E-9	45.0	6
CGCTAAG	40	6.8157533E-9	45.0	1
CCGAACG	35	1.2120836E-7	45.0	1
GCGTAAG	40	6.8157533E-9	45.0	1
ATTACCG	50	2.1827873E-11	44.999996	1
ATCACCG	60	0.0	44.999996	1
AGACCCG	55	1.8189894E-12	44.999996	1
ACGTCCA	25	3.891069E-5	44.999996	24
TAGTGCG	25	3.891069E-5	44.999996	1
ACGGTAC	25	3.891069E-5	44.999996	5
AAGGGCG	160	0.0	42.1875	5
TATCGCG	70	0.0	41.785713	1
AATTCCG	65	0.0	41.53846	1
CGCTACG	55	6.184564E-11	40.90909	1
GCGTACG	55	6.184564E-11	40.90909	1
GAACGTA	50	1.0822987E-9	40.499996	9
>>END_MODULE