FastQCFastQC Report
Sat 18 Jun 2016
SRR3554297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125397
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41060.3648490266101651No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA39830.35391955016762977No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20430.18153593798455123No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16500.14661492788766986No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13120.11658108205371083No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12490.11098305753436342No Hit
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT12120.10769532884839751Illumina Single End Adapter 2 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.0332224E-445.00000443
CATTGCG406.8157533E-945.0000041
CACTCGA351.2119017E-745.00000417
AACTCGA207.0332224E-445.00000419
GCGTAAG406.8157533E-945.0000041
AAGTCGA207.0332224E-445.00000429
TAGGTCG253.8907543E-545.013
CGATTTA253.8907543E-545.010
CGATAAG502.1827873E-1145.01
TCGAACA253.8907543E-545.044
CGACGAA302.1654614E-644.9999967
CCGAACG302.1654614E-644.9999961
GCTAACG302.1654614E-644.9999961
GTAACGG1350.043.3333322
CGCATAG650.041.538461
ACGCGAG603.6379788E-1241.2499961
TAACGCG603.6379788E-1241.2499961
TAGTGCG603.6379788E-1241.2499961
ATTACGG1750.041.1428572
CATTACG501.0822987E-940.51