##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1125397
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34900306291913	34.0	31.0	34.0	30.0	34.0
2	32.52247517986986	34.0	31.0	34.0	31.0	34.0
3	32.61429877634293	34.0	31.0	34.0	31.0	34.0
4	36.10953290261126	37.0	35.0	37.0	35.0	37.0
5	35.95552947093337	37.0	35.0	37.0	35.0	37.0
6	35.937327005492286	37.0	35.0	37.0	35.0	37.0
7	35.98321392361984	37.0	35.0	37.0	35.0	37.0
8	36.10122827766557	37.0	35.0	37.0	35.0	37.0
9	37.717711172146366	39.0	38.0	39.0	35.0	39.0
10	37.32831347515588	39.0	37.0	39.0	34.0	39.0
11	37.30640742777882	39.0	37.0	39.0	34.0	39.0
12	37.287119123296044	39.0	37.0	39.0	34.0	39.0
13	37.26139931064326	39.0	37.0	39.0	34.0	39.0
14	38.53178744922903	40.0	38.0	41.0	34.0	41.0
15	38.52879739327544	40.0	38.0	41.0	34.0	41.0
16	38.519162571074915	40.0	38.0	41.0	34.0	41.0
17	38.48557797826012	40.0	38.0	41.0	34.0	41.0
18	38.426470836513694	40.0	38.0	41.0	34.0	41.0
19	38.44142111628163	40.0	38.0	41.0	34.0	41.0
20	38.36201802563895	40.0	38.0	41.0	34.0	41.0
21	38.3168108676316	40.0	38.0	41.0	34.0	41.0
22	38.24661874876155	40.0	37.0	41.0	34.0	41.0
23	38.178935078021354	40.0	37.0	41.0	34.0	41.0
24	38.161103148488934	40.0	37.0	41.0	34.0	41.0
25	38.101070111258515	40.0	37.0	41.0	33.0	41.0
26	38.030124480516655	40.0	37.0	41.0	33.0	41.0
27	37.96342890553289	40.0	37.0	41.0	33.0	41.0
28	37.8567376667967	40.0	37.0	41.0	33.0	41.0
29	37.791121710827376	40.0	37.0	41.0	33.0	41.0
30	37.76829421084293	40.0	37.0	41.0	33.0	41.0
31	37.69303810122117	40.0	37.0	41.0	33.0	41.0
32	37.643210351547054	40.0	36.0	41.0	33.0	41.0
33	37.547059393263	40.0	36.0	41.0	33.0	41.0
34	37.45941298937175	40.0	36.0	41.0	32.0	41.0
35	37.42083371468024	40.0	36.0	41.0	32.0	41.0
36	37.34452553187897	40.0	36.0	41.0	32.0	41.0
37	37.28108303114367	39.0	36.0	41.0	32.0	41.0
38	37.235959399216455	39.0	35.0	41.0	32.0	41.0
39	37.17666032520079	39.0	35.0	41.0	32.0	41.0
40	37.084729211113945	39.0	35.0	41.0	31.0	41.0
41	36.98997864753505	39.0	35.0	41.0	31.0	41.0
42	36.922280759589725	39.0	35.0	41.0	31.0	41.0
43	36.8510641133751	39.0	35.0	41.0	31.0	41.0
44	36.76101677896778	39.0	35.0	41.0	31.0	41.0
45	36.62174326037834	39.0	35.0	41.0	31.0	41.0
46	36.48351559494116	39.0	35.0	40.0	30.0	41.0
47	36.38685192869716	39.0	35.0	40.0	30.0	41.0
48	36.29581383280745	38.0	35.0	40.0	30.0	41.0
49	36.19296568233255	38.0	35.0	40.0	30.0	41.0
50	36.09357231270387	38.0	35.0	40.0	29.0	41.0
51	35.065992711905224	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	16.0
11	12.0
12	20.0
13	16.0
14	31.0
15	45.0
16	83.0
17	146.0
18	240.0
19	443.0
20	686.0
21	1200.0
22	1668.0
23	2414.0
24	3314.0
25	4429.0
26	5744.0
27	7410.0
28	9652.0
29	12454.0
30	16050.0
31	21265.0
32	28262.0
33	38591.0
34	60684.0
35	76315.0
36	94061.0
37	137957.0
38	234742.0
39	367272.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.487436877830667	34.27261668548965	20.951006622551862	24.288939814127815
2	22.094691917607744	32.36751119827048	20.671993971905025	24.865802912216754
3	21.281378926725413	31.585031771010584	21.76769619965221	25.365893102611793
4	21.922841450616982	30.86955092291876	20.060476436315362	27.147131190148897
5	19.567317133420474	34.3804008718701	17.674118555496417	28.378163439213004
6	21.510009356698127	36.81625239804265	19.554965936465088	22.118772308794142
7	84.44264557307332	6.305419332022389	5.095446318054873	4.156488776849414
8	85.80420953672349	5.798575969191316	4.573674889838875	3.823539604246324
9	75.86682743956132	11.77291213678373	7.244465730759901	5.11579469289504
10	32.34734053849442	39.31608134729345	14.534693090527165	13.801885023684976
11	24.75082126574	28.745322761656556	28.624387660532243	17.879468312071207
12	27.40757261659663	24.60020774890994	29.279356529295885	18.712863105197545
13	24.078791750822155	26.550186289815947	28.263448365332412	21.107573594029486
14	20.42434803007294	28.344397577032815	28.916906656051154	22.31434773684309
15	20.146312812278687	30.071788000145727	29.458671028979104	20.323228158596475
16	22.118061448537716	29.137984195799348	28.641359449154386	20.102594906508546
17	21.399115156695814	29.592490472251125	28.91975009707685	20.088644273976204
18	21.245747056372107	29.814278872255745	27.582177667081037	21.357796404291108
19	22.061370343087816	30.06592340303022	27.605813770607174	20.26689248327479
20	23.374684666833126	28.936011025442575	28.605638721269028	19.08366558645527
21	23.335587352729746	30.044508737805415	26.904550127643844	19.715353781820994
22	21.48859469147332	30.287889518098947	27.728348307308444	20.49516748311929
23	22.042799118888713	29.606352247251415	27.51597880570145	20.83486982815842
24	20.52973306308796	28.86421413954365	29.700185801099522	20.90586699626887
25	20.182477827824314	30.884390130771628	26.93849370488814	21.994638336515916
26	21.489927554454116	30.844581956411826	26.853279331649187	20.81221115748487
27	21.1065961611769	30.094180098223116	27.284593792235096	21.514629948364888
28	21.58580483153945	29.94676545254697	28.916640083454993	19.55078963245859
29	21.104463580407625	30.062280244216037	27.23083498534295	21.602421190033382
30	21.688346423528763	30.0710771398893	27.652908262595332	20.5876681739866
31	21.27196002832778	30.256700524348297	26.89841895793218	21.572920489391745
32	22.825900548872976	29.991194218573536	27.361011269800787	19.8218939627527
33	20.703804968380048	31.0975593501671	27.83568820602863	20.362947475424228
34	19.9650434468903	29.870614547577436	29.532156207987047	20.632185797545223
35	21.476865497242308	28.963468002846994	29.109727500606454	20.449938999304244
36	21.205938882012305	31.636657997133455	27.30849646835739	19.848906652496854
37	20.899202681364887	32.58681158737761	27.21635120761829	19.29763452363921
38	20.132273322214296	30.76372160224348	29.01855967271994	20.085445402822295
39	20.9774861671037	30.05748193748517	28.896913711339202	20.06811818407193
40	22.264676376425385	29.35906173554754	29.009051916790252	19.367209971236818
41	19.258537209535834	29.748968586196696	30.189968517776393	20.802525686491077
42	19.844996921086512	30.249325349187888	27.782817974457014	22.122859755268586
43	20.77737900492004	30.10031126793478	27.948803844332264	21.17350588281291
44	21.52715886038438	30.348134924830973	26.78441474430801	21.34029147047664
45	20.788841626554895	30.247725913610928	27.81445125586793	21.148981203966244
46	18.553008405033957	30.7814931086541	29.231906607179507	21.43359187913243
47	20.116989826701158	30.23217584550163	28.828759984254443	20.822074343542766
48	20.320029287442566	28.657975807648324	29.54184167898084	21.480153225928273
49	19.110500561135314	30.74204036442251	28.430589383124357	21.716869691317818
50	18.728768603435054	30.003101127868653	29.304325495802814	21.96380477289348
51	19.86276842749714	29.032954592912546	28.558721944344974	22.54555503524534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5503.0
1	4256.0
2	3009.0
3	2192.0
4	1375.0
5	1379.5
6	1384.0
7	1661.5
8	1939.0
9	2302.5
10	2666.0
11	3244.5
12	3823.0
13	4155.0
14	4487.0
15	4675.5
16	4864.0
17	5061.0
18	5258.0
19	5403.5
20	5549.0
21	6755.5
22	7962.0
23	10938.5
24	13915.0
25	12706.0
26	14830.5
27	18164.0
28	21393.0
29	24622.0
30	28860.0
31	33098.0
32	39470.5
33	45843.0
34	48123.0
35	50403.0
36	53354.5
37	56306.0
38	57742.5
39	59179.0
40	60997.0
41	62815.0
42	64751.5
43	66688.0
44	69535.5
45	72383.0
46	75892.5
47	79402.0
48	80043.5
49	80685.0
50	77309.5
51	73934.0
52	68068.0
53	62202.0
54	57078.5
55	51955.0
56	48032.0
57	44109.0
58	42575.5
59	41042.0
60	39079.0
61	37116.0
62	33823.5
63	30531.0
64	26780.0
65	23029.0
66	18615.5
67	14202.0
68	11862.5
69	9523.0
70	7590.0
71	5657.0
72	4690.5
73	3724.0
74	3014.0
75	1956.0
76	1608.0
77	1226.0
78	844.0
79	629.5
80	415.0
81	325.0
82	235.0
83	149.5
84	64.0
85	43.0
86	22.0
87	23.0
88	24.0
89	20.5
90	17.0
91	10.5
92	4.0
93	3.5
94	3.0
95	6.5
96	10.0
97	7.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1125397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.40682449121783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.348189048347	16.93441363312106
2	8.85581530858272	4.145730293092715
3	3.7270420985592656	2.617146608170707
4	2.160419498469789	2.022742401123488
5	1.5132394800211988	1.7710065461018962
6	1.0982729561219529	1.5424249396438516
7	0.8690520557913629	1.4239224260548333
8	0.7245302908267673	1.3567162684762522
9	0.5894880334419734	1.2418238644604476
>10	6.6965726536781105	34.59495986556257
>50	1.0121404663792248	16.472392852096394
>100	0.3983631936862624	13.798528490851378
>500	0.004201337613182015	0.6875752052086688
>1k	0.002673578481115828	1.3906166060357354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4106	0.3648490266101651	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3983	0.35391955016762977	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2043	0.18153593798455123	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1650	0.14661492788766986	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1312	0.11658108205371083	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1249	0.11098305753436342	No Hit
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	1212	0.10769532884839751	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12919885160525574	0.0
2	0.0	0.0	0.0	0.19610857324126507	0.0
3	0.0	0.0	0.0	0.28025665609558226	0.0
4	0.0	0.0	0.0	0.6506148496930416	0.0
5	0.0	0.0	0.0	0.7323637791819242	0.0
6	0.0	0.0	0.0	1.2011761182942553	0.0
7	0.0	0.0	0.0	1.5348361511537707	0.0
8	0.0	0.0	0.0	1.8588107130194944	0.0
9	0.0	0.0	0.0	2.328511627452357	0.0
10	0.0	0.0	0.0	3.0679840091985318	0.0
11	0.0	0.0	0.0	3.6922081718717927	0.0
12	0.0	0.0	0.0	3.9899697617818424	0.0
13	0.0	0.0	0.0	4.143782149765816	0.0
14	0.0	0.0	0.0	4.310034592237228	0.0
15	0.0	0.0	0.0	4.4346128521757215	0.0
16	0.0	0.0	0.0	4.6532023810264285	0.0
17	0.0	0.0	0.0	4.917020393692182	0.0
18	0.0	0.0	0.0	5.230243194179476	0.0
19	0.0	0.0	0.0	5.410623984247337	0.0
20	0.0	0.0	0.0	5.5971359440268635	0.0
21	0.0	0.0	0.0	5.813592892108296	0.0
22	0.0	0.0	0.0	6.049509639709365	0.0
23	0.0	0.0	0.0	6.27938407513082	0.0
24	0.0	0.0	0.0	6.465896034910347	0.0
25	0.0	0.0	0.0	6.640412227862701	0.0
26	0.0	0.0	0.0	6.809952399020079	0.0
27	0.0	0.0	0.0	6.98553488235707	0.0
28	0.0	0.0	0.0	7.159873360245318	0.0
29	0.0	0.0	0.0	7.35304963492883	0.0
30	0.0	0.0	0.0	7.596074985094149	0.0
31	0.0	0.0	0.0	7.794227281572637	0.0
32	8.885753205313325E-5	0.0	0.0	8.013794243275928	0.0
33	8.885753205313325E-5	0.0	0.0	8.216389416357073	0.0
34	8.885753205313325E-5	0.0	0.0	8.415074858027879	0.0
35	8.885753205313325E-5	0.0	0.0	8.62726664457076	0.0
36	8.885753205313325E-5	0.0	0.0	8.857585367652481	0.0
37	8.885753205313325E-5	0.0	0.0	9.080706630637899	0.0
38	8.885753205313325E-5	0.0	0.0	9.303561321027157	0.0
39	8.885753205313325E-5	0.0	0.0	9.53361347151272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.0332224E-4	45.000004	43
CATTGCG	40	6.8157533E-9	45.000004	1
CACTCGA	35	1.2119017E-7	45.000004	17
AACTCGA	20	7.0332224E-4	45.000004	19
GCGTAAG	40	6.8157533E-9	45.000004	1
AAGTCGA	20	7.0332224E-4	45.000004	29
TAGGTCG	25	3.8907543E-5	45.0	13
CGATTTA	25	3.8907543E-5	45.0	10
CGATAAG	50	2.1827873E-11	45.0	1
TCGAACA	25	3.8907543E-5	45.0	44
CGACGAA	30	2.1654614E-6	44.999996	7
CCGAACG	30	2.1654614E-6	44.999996	1
GCTAACG	30	2.1654614E-6	44.999996	1
GTAACGG	135	0.0	43.333332	2
CGCATAG	65	0.0	41.53846	1
ACGCGAG	60	3.6379788E-12	41.249996	1
TAACGCG	60	3.6379788E-12	41.249996	1
TAGTGCG	60	3.6379788E-12	41.249996	1
ATTACGG	175	0.0	41.142857	2
CATTACG	50	1.0822987E-9	40.5	1
>>END_MODULE