FastQCFastQC Report
Sat 18 Jun 2016
SRR3554296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025989
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47590.4638451289438776No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA34440.3356761134865968No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29510.2876249160566049No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22300.21735125815189055No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT22200.21637658883282376No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22110.21549938644566366No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA13860.13508916762265483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG351.2117562E-745.0000041
CATACGC351.2117562E-745.00000415
ATACGCG650.045.0000041
TCGTTAT207.032932E-445.034
CTCGTTA207.032932E-445.033
ATCGTCA207.032932E-445.022
GTTACGA207.032932E-445.012
CGTAGTA253.8905124E-545.042
GTTCGAC207.032932E-445.037
CGCTATA207.032932E-445.01
CGTAGCG406.8139343E-945.01
GTCCGTT207.032932E-445.028
TCGTAAG207.032932E-445.01
CGGTCTA453.8380676E-1045.031
TAAATCG207.032932E-445.019
TATCGCG406.8139343E-945.01
ATCGCAT207.032932E-445.033
TAGTCCG207.032932E-445.01
TACCGGA406.8139343E-945.03
TACGCGG1550.042.0967752