##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1025989
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30622745468031	34.0	31.0	34.0	30.0	34.0
2	32.4967002570203	34.0	31.0	34.0	30.0	34.0
3	32.59340109884219	34.0	31.0	34.0	31.0	34.0
4	36.099202817963935	37.0	35.0	37.0	35.0	37.0
5	35.93767282105364	37.0	35.0	37.0	35.0	37.0
6	35.908232934271226	37.0	35.0	37.0	35.0	37.0
7	35.91863070656703	37.0	35.0	37.0	35.0	37.0
8	36.03283173601277	37.0	35.0	37.0	35.0	37.0
9	37.676374697974346	39.0	37.0	39.0	35.0	39.0
10	37.329424584474104	39.0	37.0	39.0	34.0	39.0
11	37.27193761336623	39.0	37.0	39.0	34.0	39.0
12	37.14857956566786	39.0	37.0	39.0	34.0	39.0
13	37.10508007395791	39.0	37.0	39.0	33.0	39.0
14	38.36273098444525	40.0	38.0	41.0	34.0	41.0
15	38.37995631532112	40.0	38.0	41.0	34.0	41.0
16	38.36597175993115	40.0	38.0	41.0	34.0	41.0
17	38.3446118818038	40.0	38.0	41.0	34.0	41.0
18	38.27814040891277	40.0	37.0	41.0	34.0	41.0
19	38.28472430016306	40.0	37.0	41.0	34.0	41.0
20	38.19807912170598	40.0	37.0	41.0	34.0	41.0
21	38.15019069405228	40.0	37.0	41.0	33.0	41.0
22	38.09973108873487	40.0	37.0	41.0	33.0	41.0
23	38.04786308625141	40.0	37.0	41.0	33.0	41.0
24	37.95458138440081	40.0	37.0	41.0	33.0	41.0
25	37.87950163208377	40.0	37.0	41.0	33.0	41.0
26	37.78849675776251	40.0	37.0	41.0	33.0	41.0
27	37.70613622563205	40.0	36.0	41.0	33.0	41.0
28	37.65683062878842	40.0	36.0	41.0	33.0	41.0
29	37.54085959985926	40.0	36.0	41.0	33.0	41.0
30	37.52080382928082	40.0	36.0	41.0	33.0	41.0
31	37.47903924895881	40.0	36.0	41.0	32.0	41.0
32	37.40364955179832	40.0	36.0	41.0	32.0	41.0
33	37.29660844317044	40.0	36.0	41.0	32.0	41.0
34	37.14863317248041	39.0	35.0	41.0	31.0	41.0
35	37.138867960572675	39.0	35.0	41.0	31.0	41.0
36	37.031526653794536	39.0	35.0	41.0	31.0	41.0
37	36.98464213553947	39.0	35.0	41.0	31.0	41.0
38	36.92277694985034	39.0	35.0	41.0	31.0	41.0
39	36.85051204252677	39.0	35.0	41.0	31.0	41.0
40	36.754914526374066	39.0	35.0	41.0	31.0	41.0
41	36.653332540602285	39.0	35.0	41.0	31.0	41.0
42	36.563048921577135	39.0	35.0	41.0	30.0	41.0
43	36.495597906020436	39.0	35.0	41.0	30.0	41.0
44	36.38991646109266	39.0	35.0	40.0	30.0	41.0
45	36.25247151772582	39.0	35.0	40.0	30.0	41.0
46	36.144562953403984	38.0	35.0	40.0	29.0	41.0
47	36.01671752816063	38.0	35.0	40.0	29.0	41.0
48	35.90707307778153	38.0	35.0	40.0	29.0	41.0
49	35.75947695345661	38.0	34.0	40.0	28.0	41.0
50	35.608872024943736	38.0	34.0	40.0	28.0	41.0
51	34.53798627470665	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	8.0
12	7.0
13	11.0
14	29.0
15	40.0
16	87.0
17	158.0
18	278.0
19	497.0
20	830.0
21	1275.0
22	1943.0
23	2749.0
24	3831.0
25	4903.0
26	6286.0
27	8159.0
28	10151.0
29	13000.0
30	16853.0
31	21652.0
32	27970.0
33	38155.0
34	60154.0
35	74984.0
36	85467.0
37	124441.0
38	207539.0
39	314388.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.7987610003616	32.75288526485177	20.21590874755967	26.23244498722696
2	23.295668861946865	32.15092949339613	19.984619718145126	24.568781926511882
3	22.51018285771095	31.645465984528098	20.130235314413703	25.714115843347248
4	23.028999336250195	30.721576936984707	19.28168820523417	26.967735521530933
5	19.690659451514588	34.08993663674757	16.70427265789399	29.51513125384385
6	20.983850704052383	38.693104896836125	18.774372824659913	21.54867157445158
7	83.05449668563698	7.47259473542114	4.965940180645212	4.506968398296668
8	84.8166013475778	6.728337243381752	4.6535586638843105	3.801502745156137
9	74.6016770160304	12.417676992638322	7.785658520705388	5.194987470625904
10	36.58167875094178	35.28839003147207	14.60990322508331	13.520027992502845
11	28.23197909529244	29.530336095221294	24.586131040391273	17.651553769094992
12	30.221766510167264	25.836241909026313	26.368118956441055	17.573872624365368
13	24.67502088229016	27.580510122428215	26.26880015282815	21.475668842453477
14	21.40900146102931	28.78042552113132	27.7453267042824	22.06524631355697
15	22.015245777488843	29.9464224275309	28.623698694625382	19.414633100354877
16	23.456586766524786	30.078002785604912	27.048048273422037	19.417362174448265
17	22.281525435457887	30.115527554388983	27.401268434651833	20.201678575501298
18	21.777913798296083	30.747308207008068	26.810813761161185	20.663964233534667
19	24.060004541959028	30.0545132550154	26.320750027534405	19.56473217549116
20	25.496179783603917	29.066003631617882	26.91997672489666	18.517839859881537
21	23.63465885111829	31.151698507488874	26.783815421023032	18.42982722036981
22	23.267208517830113	30.207926205836515	26.510225743160987	20.01463953317238
23	24.348701594266604	29.262886834069374	26.507106801339976	19.88130477032405
24	22.36817353792292	29.292516781369	28.619410149621487	19.71989953108659
25	21.684053142869953	32.450542842077255	25.379219465315906	20.486184549736887
26	22.624803969633202	31.092925947549144	26.011877320322146	20.270392762495504
27	21.106659038254797	30.50578514974332	27.022901804990113	21.364654007011772
28	21.935127959461553	31.275384044078447	26.968027922326655	19.82146007413335
29	21.47615617711301	31.43367034149489	25.410701284321764	21.67947219707034
30	21.23092937643581	31.058617587517993	26.828455275836287	20.881997760209906
31	23.243329119512975	30.48843603586393	25.9440403357151	20.32419450890799
32	24.063513351507666	30.466311042321117	25.98107776983964	19.489097836331577
33	23.169546652059623	31.754726415195485	25.41606196557663	19.659664967168265
34	21.102467960182807	31.1010157028974	27.86813503848482	19.928381298434974
35	23.347521269721216	29.920203822848002	27.231383572338498	19.500891335092284
36	21.77703659590892	34.15348507635072	25.10855379541106	18.960924532329294
37	21.868850445765013	33.54685089216356	26.38790474361811	18.196393918453317
38	21.4307365868445	31.27928272135471	27.38655092793392	19.903429763866864
39	23.053073668431143	30.09603416800765	26.637809957026832	20.213082206534377
40	22.352383894954038	30.30246912978599	27.685482008091704	19.659664967168265
41	19.982475445643182	30.204514863219785	29.396416530781522	20.41659316035552
42	20.27516864215893	30.245450974620585	27.227387428130324	22.25199295509016
43	21.072935479815087	30.156658599653603	27.591036551074133	21.179369369457177
44	21.901404401021843	29.664937928184415	26.1965771562853	22.23708051450844
45	21.407052122391175	30.256659671789855	27.053116553881186	21.28317165193779
46	19.445725051633104	31.903850821012703	27.524661570445687	21.125762556908505
47	21.40373824670635	29.970594226643755	27.54434989069083	21.08131763595906
48	20.961530776645752	28.492118336551364	29.15898708465685	21.387363802146027
49	20.396222571587025	31.6600860243141	27.15058348578786	20.793107918311016
50	20.26415487885348	30.239115624046654	28.24698900280607	21.249740494293796
51	20.22273143279314	29.235888493931224	28.38734138475169	22.15403868852395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6335.0
1	4849.5
2	3364.0
3	2343.5
4	1323.0
5	1275.0
6	1227.0
7	1386.5
8	1546.0
9	1704.0
10	1862.0
11	2180.0
12	2498.0
13	2683.0
14	2868.0
15	2880.0
16	2892.0
17	3317.0
18	3742.0
19	4362.5
20	4983.0
21	5206.0
22	5429.0
23	8119.5
24	10810.0
25	11852.5
26	13790.5
27	14686.0
28	19759.0
29	24832.0
30	29313.0
31	33794.0
32	36785.5
33	39777.0
34	41916.5
35	44056.0
36	45097.0
37	46138.0
38	49779.5
39	53421.0
40	55271.0
41	57121.0
42	59143.0
43	61165.0
44	62780.5
45	64396.0
46	65304.0
47	66212.0
48	65957.5
49	65703.0
50	63557.0
51	61411.0
52	57611.5
53	53812.0
54	50476.0
55	47140.0
56	44712.5
57	42285.0
58	41357.0
59	40429.0
60	40531.5
61	40634.0
62	37860.0
63	35086.0
64	29247.5
65	23409.0
66	20118.0
67	16827.0
68	13768.5
69	10710.0
70	9219.5
71	7729.0
72	6523.5
73	5318.0
74	4315.0
75	2778.5
76	2245.0
77	1804.5
78	1364.0
79	960.0
80	556.0
81	435.0
82	314.0
83	242.0
84	170.0
85	119.5
86	69.0
87	63.5
88	58.0
89	41.0
90	24.0
91	13.0
92	2.0
93	3.5
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1025989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.600663277631284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70909686144448	16.137660450800023
2	8.215961821967145	3.549404496363727
3	3.1374373215853666	2.033121814147166
4	1.746960634427262	1.5094203369416164
5	1.1583888147289296	1.2510983365767012
6	0.8577481401873718	1.1116757251921139
7	0.7117503704129655	1.0761996062313866
8	0.5908952021423455	1.0210982635075743
9	0.4838425047247331	0.9406187121568217
>10	6.687830093582263	32.899533715168836
>50	1.11752466637346	16.78830199597476
>100	0.5721363605841898	18.696724599790805
>500	0.0072537098013843405	1.1048247038588326
>1k	0.003173498038105649	1.8803172432896156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4759	0.4638451289438776	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3444	0.3356761134865968	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2951	0.2876249160566049	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2230	0.21735125815189055	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	2220	0.21637658883282376	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2211	0.21549938644566366	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1386	0.13508916762265483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09717453111095734	0.0
2	9.746693190667736E-5	0.0	0.0	0.13967011342226865	0.0
3	9.746693190667736E-5	0.0	0.0	0.18820864551179398	0.0
4	9.746693190667736E-5	0.0	0.0	0.4309987728913273	0.0
5	9.746693190667736E-5	0.0	0.0	0.47651583009174564	0.0
6	9.746693190667736E-5	0.0	0.0	0.8205740997223168	0.0
7	9.746693190667736E-5	0.0	0.0	1.0804209401855185	0.0
8	9.746693190667736E-5	0.0	0.0	1.3370513718958001	0.0
9	9.746693190667736E-5	0.0	0.0	1.6487506201333542	0.0
10	9.746693190667736E-5	0.0	0.0	2.135500478075301	0.0
11	9.746693190667736E-5	0.0	0.0	2.581314224616443	0.0
12	9.746693190667736E-5	0.0	0.0	2.790088392760546	0.0
13	9.746693190667736E-5	0.0	0.0	2.8898945310329838	0.0
14	9.746693190667736E-5	0.0	0.0	3.0114357951206103	0.0
15	9.746693190667736E-5	0.0	0.0	3.107343256116781	0.0
16	9.746693190667736E-5	0.0	0.0	3.287072278552694	0.0
17	9.746693190667736E-5	0.0	0.0	3.481616274638422	0.0
18	9.746693190667736E-5	0.0	0.0	3.709688895300047	0.0
19	9.746693190667736E-5	0.0	0.0	3.8309377585919537	0.0
20	9.746693190667736E-5	0.0	0.0	3.9693408018994356	0.0
21	9.746693190667736E-5	0.0	0.0	4.135132053072693	0.0
22	9.746693190667736E-5	0.0	0.0	4.324315367903554	0.0
23	9.746693190667736E-5	0.0	0.0	4.501607717041801	0.0
24	9.746693190667736E-5	0.0	0.0	4.658042142752018	0.0
25	9.746693190667736E-5	0.0	0.0	4.790694637077006	0.0
26	9.746693190667736E-5	0.0	0.0	4.921300325831953	0.0
27	9.746693190667736E-5	0.0	0.0	5.056194559590795	0.0
28	9.746693190667736E-5	0.0	0.0	5.194305202102557	0.0
29	9.746693190667736E-5	0.0	0.0	5.34294227326024	0.0
30	9.746693190667736E-5	0.0	0.0	5.5207219570580195	0.0
31	1.9493386381335472E-4	0.0	0.0	5.673647573219596	0.0
32	1.9493386381335472E-4	0.0	0.0	5.841875497690521	0.0
33	1.9493386381335472E-4	0.0	0.0	5.9947036469201915	0.0
34	1.9493386381335472E-4	0.0	0.0	6.1610796996848896	0.0
35	1.9493386381335472E-4	0.0	0.0	6.338956850414576	0.0
36	1.9493386381335472E-4	0.0	0.0	6.538179259231824	0.0
37	1.9493386381335472E-4	0.0	0.0	6.719662686442057	0.0
38	1.9493386381335472E-4	0.0	0.0	6.926292582084213	0.0
39	1.9493386381335472E-4	0.0	0.0	7.143643840236104	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATG	35	1.2117562E-7	45.000004	1
CATACGC	35	1.2117562E-7	45.000004	15
ATACGCG	65	0.0	45.000004	1
TCGTTAT	20	7.032932E-4	45.0	34
CTCGTTA	20	7.032932E-4	45.0	33
ATCGTCA	20	7.032932E-4	45.0	22
GTTACGA	20	7.032932E-4	45.0	12
CGTAGTA	25	3.8905124E-5	45.0	42
GTTCGAC	20	7.032932E-4	45.0	37
CGCTATA	20	7.032932E-4	45.0	1
CGTAGCG	40	6.8139343E-9	45.0	1
GTCCGTT	20	7.032932E-4	45.0	28
TCGTAAG	20	7.032932E-4	45.0	1
CGGTCTA	45	3.8380676E-10	45.0	31
TAAATCG	20	7.032932E-4	45.0	19
TATCGCG	40	6.8139343E-9	45.0	1
ATCGCAT	20	7.032932E-4	45.0	33
TAGTCCG	20	7.032932E-4	45.0	1
TACCGGA	40	6.8139343E-9	45.0	3
TACGCGG	155	0.0	42.096775	2
>>END_MODULE