FastQCFastQC Report
Sat 18 Jun 2016
SRR3554294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646153
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35070.5427507107449783No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15800.2445241297339794No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14990.23198839903242732No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT10760.1665240275909885No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7870.12179777854471No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT7870.12179777854471TruSeq Adapter, Index 21 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG302.1640208E-645.0000041
GAATCGG253.8889026E-545.0000042
CGTTCAT253.8889026E-545.00000417
AATATCG253.8889026E-545.0000042
TAGCACG253.8889026E-545.0000041
CTACGCG600.045.0000041
ACTCGCG453.8380676E-1045.0000041
AATTACG253.8889026E-545.0000041
CGTAACG253.8889026E-545.0000041
ACGTAGG302.1640208E-645.0000042
ACCCACG253.8889026E-545.0000041
ATACACG302.1640208E-645.0000041
AACGTTC207.030992E-445.026
GTATTCG351.2108285E-745.020
CTATACG207.030992E-445.01
CAACCCG207.030992E-445.01
GCGTTGT351.2108285E-745.09
ATCGTAG207.030992E-445.01
GTAATCG207.030992E-445.09
AATTGCG850.045.01