FastQCFastQC Report
Sat 18 Jun 2016
SRR3554293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628800
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34570.5497773536895675No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15100.24013994910941477No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15090.23998091603053434No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT10810.1719147582697201No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT8030.12770356234096694TruSeq Adapter, Index 21 (95% over 22bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7820.12436386768447838No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.030846E-445.0000041
CGTGAGT207.030846E-445.00000411
CGACACG406.8066583E-945.0000041
GTCGTTA207.030846E-445.0000049
CGCAGTA253.8887825E-545.00000426
GTATACG207.030846E-445.0000041
CAATGCG253.8887825E-545.0000041
CAGTACG207.030846E-445.0000041
AATACCG253.8887825E-545.0000041
CCGTTCC207.030846E-445.00000417
AGTAACG253.8887825E-545.0000041
AATCGAG253.8887825E-545.0000041
TACCGGA253.8887825E-545.0000043
GATACGC351.2107557E-745.011
GCATGCG453.8380676E-1045.01
CCAATCG302.163928E-644.99999619
TCGATAT302.163928E-644.99999644
AATTGCG551.8189894E-1244.9999961
ACTAACG302.163928E-644.9999961
ATACGAG600.044.9999961