FastQCFastQC Report
Sat 18 Jun 2016
SRR3554292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653387
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51070.7816194690130045No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT15760.24120467655462996Illumina PCR Primer Index 5 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9420.1441718307832877No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8360.12794867360385193No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA7440.11386819756132277No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG7110.10881759202432861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA302.1640572E-645.00000420
GCGGTTC207.0310483E-445.00000435
CTATCGT207.0310483E-445.00000414
ACACCGT207.0310483E-445.00000432
GACGTAG406.8066583E-945.0000041
ATAGCCG406.8066583E-945.0000041
CGATTGG207.0310483E-445.0000042
CGATTAG207.0310483E-445.0000041
TAGCCCG207.0310483E-445.0000041
ATCCGTT207.0310483E-445.00000420
GTAATCG207.0310483E-445.0000049
ATATCCG406.8066583E-945.0000041
CTACGTT207.0310483E-445.00000412
CGTAGCG302.1640572E-645.0000041
AGACCGT302.1640572E-645.00000414
CAGTGCG302.1640572E-645.0000041
ACGCTAA207.0310483E-445.00000411
TTACGCG351.2108467E-745.0000041
CGTCGAG207.0310483E-445.0000041
CAATGCG207.0310483E-445.0000041