FastQCFastQC Report
Sat 18 Jun 2016
SRR3554289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340535
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54851.610700809021099No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT10660.31303683909142965No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10510.30863200552072473No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8570.2516628246729411No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.1435975744049804No Hit
AAGGGAGGGCATGCAAAAACTAACATTGAAATTTTGAAACAGCAGCAGAGT3640.1068906279824394No Hit
AAAGCAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGC3640.1068906279824394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC351.2085547E-745.0000046
GATACGT253.8850034E-545.00000412
GGTACCC253.8850034E-545.0000049
TTGGACC253.8850034E-545.00000419
TCCGATA253.8850034E-545.00000411
CCGATAT253.8850034E-545.00000412
GTTTACC253.8850034E-545.00000410
ATTACGG700.045.0000042
TAGCGCA253.8850034E-545.00000440
ATTACCG502.1827873E-1145.0000041
ATCCGAT253.8850034E-545.00000410
TACGCGG351.2085547E-745.0000042
CGCTCCG351.2085547E-745.0000041
CTAGTGA253.8850034E-545.00000430
CGAGGTT351.2085547E-745.00000429
GACGGGT253.8850034E-545.0000044
ATACGTA253.8850034E-545.00000413
CGCAAAC253.8850034E-545.00000424
TCCCACG253.8850034E-545.0000041
CGATAGG253.8850034E-545.0000042