##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340535
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2377200581438	33.0	31.0	34.0	30.0	34.0
2	32.41188717752947	34.0	31.0	34.0	30.0	34.0
3	32.49778730526965	34.0	31.0	34.0	30.0	34.0
4	36.022743624003404	37.0	35.0	37.0	35.0	37.0
5	35.866821325267594	37.0	35.0	37.0	35.0	37.0
6	35.84769260134788	37.0	35.0	37.0	35.0	37.0
7	35.824728735665936	37.0	35.0	37.0	35.0	37.0
8	35.964696727208654	37.0	35.0	37.0	35.0	37.0
9	37.63145344825055	39.0	37.0	39.0	35.0	39.0
10	37.22920698312949	39.0	37.0	39.0	34.0	39.0
11	37.238369036956556	39.0	37.0	39.0	34.0	39.0
12	37.18754019410633	39.0	37.0	39.0	34.0	39.0
13	37.17158882346895	39.0	37.0	39.0	33.0	39.0
14	38.40382339553937	40.0	38.0	41.0	34.0	41.0
15	38.410380724448295	40.0	38.0	41.0	34.0	41.0
16	38.39388021789243	40.0	38.0	41.0	34.0	41.0
17	38.35322947714626	40.0	38.0	41.0	34.0	41.0
18	38.24203679504309	40.0	37.0	41.0	34.0	41.0
19	38.16913679944793	40.0	37.0	41.0	34.0	41.0
20	38.0880966714141	40.0	37.0	41.0	33.0	41.0
21	38.03217877751186	40.0	37.0	41.0	33.0	41.0
22	37.99370402454961	40.0	37.0	41.0	33.0	41.0
23	37.855333519315195	40.0	37.0	41.0	33.0	41.0
24	37.81069493590967	40.0	36.0	41.0	33.0	41.0
25	37.75297106024344	40.0	36.0	41.0	33.0	41.0
26	37.70805937715653	40.0	36.0	41.0	33.0	41.0
27	37.671781167868204	40.0	36.0	41.0	33.0	41.0
28	37.58279765662854	40.0	36.0	41.0	33.0	41.0
29	37.51257873640007	40.0	36.0	41.0	33.0	41.0
30	37.47464724624488	40.0	36.0	41.0	33.0	41.0
31	37.392171142467	40.0	36.0	41.0	32.0	41.0
32	37.33812677111016	40.0	36.0	41.0	32.0	41.0
33	37.23628995551118	39.0	36.0	41.0	32.0	41.0
34	37.14941195471831	39.0	35.0	41.0	31.0	41.0
35	37.02441158764885	39.0	35.0	41.0	31.0	41.0
36	36.988177426696225	39.0	35.0	41.0	31.0	41.0
37	36.90699047087671	39.0	35.0	41.0	31.0	41.0
38	36.86759363942032	39.0	35.0	41.0	31.0	41.0
39	36.784389269825425	39.0	35.0	41.0	31.0	41.0
40	36.71742405332785	39.0	35.0	41.0	31.0	41.0
41	36.64665306062519	39.0	35.0	41.0	30.0	41.0
42	36.56356615325884	39.0	35.0	41.0	30.0	41.0
43	36.496139897514205	39.0	35.0	41.0	30.0	41.0
44	36.420276917203815	39.0	35.0	41.0	30.0	41.0
45	36.26680664248902	39.0	35.0	40.0	30.0	41.0
46	36.095708223824275	38.0	35.0	40.0	30.0	41.0
47	36.015061594256096	38.0	35.0	40.0	29.0	41.0
48	35.909213443552055	38.0	35.0	40.0	29.0	41.0
49	35.80491579426491	38.0	35.0	40.0	29.0	41.0
50	35.728861937833116	38.0	35.0	40.0	28.0	41.0
51	34.71053489362327	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	6.0
13	6.0
14	10.0
15	28.0
16	24.0
17	50.0
18	108.0
19	202.0
20	345.0
21	573.0
22	845.0
23	1068.0
24	1463.0
25	1827.0
26	2155.0
27	2732.0
28	3390.0
29	4306.0
30	5546.0
31	7009.0
32	9201.0
33	12357.0
34	18935.0
35	26622.0
36	28535.0
37	40367.0
38	69018.0
39	103754.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.365395627468544	30.73252382280823	20.693027148457574	26.209053401265653
2	23.282481976889308	32.66595210477631	21.833879043270148	22.21768687506424
3	21.320275448925955	32.792517656041234	20.7523455738764	25.134861321156414
4	20.888895414568253	33.30876415052785	20.033476735137356	25.76886369976654
5	18.00431673689929	34.44756045634076	18.644779538079785	28.903343268680164
6	20.749702673733978	38.35024299998532	19.89986345015931	21.000190876121398
7	80.77583801958683	8.425565654044371	5.83640448118402	4.962191845184783
8	82.60413760700075	8.079052079815584	4.855594872773724	4.4612154404099424
9	74.04055383440762	12.41751949138855	8.88895414568253	4.652972528521297
10	30.578648303404936	37.932664777482486	16.891362121367848	14.597324797744726
11	24.91520695376393	30.300850132879148	28.071710690531077	16.712232222825847
12	24.72021965436739	27.42155725549503	29.12505322507231	18.733169865065268
13	23.846887985082297	28.32631007091782	28.15364059494619	19.673161349053693
14	19.338100342108742	30.094703921770154	30.594505704259472	19.972690031861628
15	18.84710822676083	32.18817449014051	29.890025988518065	19.074691294580585
16	19.617660446062814	32.054561205162464	28.473431512179364	19.854346836595358
17	20.363545597368844	30.891685142496367	29.167045971779697	19.577723288355088
18	21.17315400766441	29.06955232208143	29.654220564699663	20.103073105554493
19	20.861291790858502	31.743286299499317	29.330024813895783	18.0653970957464
20	21.457118945189187	30.77950871422908	28.566520328306925	19.196852012274803
21	21.83329173212739	31.473710484972177	28.004169909113603	18.688827873786835
22	22.07996241208686	30.077378243058718	29.012876796805028	18.829782548049394
23	20.87098242471405	30.960400546199363	27.98508229697388	20.183534732112705
24	19.145168631711865	30.507877310702273	30.003083383499497	20.343870674086364
25	20.38439514293685	31.482813807684966	28.15422790608895	19.978563143289236
26	20.122454373265597	30.90255039863744	28.69367319071461	20.281322037382353
27	18.3728544789816	31.007091782048835	29.462757132159688	21.157296606809872
28	19.14722422071153	30.38160541500874	29.602830839708105	20.86833952457163
29	19.16807376627953	30.4911389431336	29.618981896134024	20.721805394452847
30	18.803647202196544	31.045560661899657	29.044004287371344	21.106787848532456
31	20.330362517802868	30.999456737192947	27.710807993304655	20.959372751699533
32	20.444594535069815	29.93201873522545	28.966772872098318	20.656613857606413
33	19.41856196866695	30.534893623269266	28.906573479965346	21.139970928098435
34	19.477293082943017	30.69875343209949	29.073957155652135	20.749996329305358
35	18.82655233676421	30.787143759084966	29.955804836507262	20.430499067643563
36	19.111691896574506	31.986726768173607	29.744079169542044	19.15750216570984
37	19.117565008002114	31.443757616691382	29.564655615428663	19.874021759877838
38	20.667772769318866	31.177999324592186	30.03714742977961	18.117080476309336
39	19.36335472124745	30.209523250179863	29.64100606398755	20.78611596458514
40	20.564699663764372	30.925161877633723	28.71041155828329	19.799726900318614
41	18.561968666950534	30.099989722055003	30.44738426299793	20.890657347996537
42	19.699003039335164	30.366335325296962	29.378771638744915	20.555889996622962
43	19.146343253997387	30.71813469981059	30.039790329922035	20.095731716269988
44	19.594461655923766	29.74114261382824	28.884255656540443	21.78014007370755
45	21.429221665908056	30.091473710484973	28.473137856607984	20.006166766998987
46	18.16553364558709	30.390415082150145	30.784794514513926	20.659256757748835
47	19.675216938053357	30.094997577341537	29.734975846829254	20.49480963777585
48	19.73013052990148	29.59607676156636	30.76893711365939	19.90485559487277
49	19.87372810430646	29.983114804645634	29.200816362488435	20.942340728559472
50	18.98072151173888	28.3251354486323	30.799477293082944	21.894665746545876
51	19.038571659300807	27.74252279501373	31.641094160658962	21.5778113850265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6366.0
1	4727.0
2	3088.0
3	1992.5
4	897.0
5	752.5
6	608.0
7	669.0
8	730.0
9	738.0
10	746.0
11	932.0
12	1118.0
13	1162.5
14	1207.0
15	1356.5
16	1506.0
17	1341.5
18	1177.0
19	1551.5
20	1926.0
21	2020.5
22	2115.0
23	2776.5
24	3438.0
25	3552.0
26	4302.5
27	4939.0
28	5748.5
29	6558.0
30	8246.0
31	9934.0
32	11669.0
33	13404.0
34	13831.5
35	14259.0
36	16773.5
37	19288.0
38	18886.5
39	18485.0
40	18889.0
41	19293.0
42	20899.0
43	22505.0
44	22377.0
45	22249.0
46	24338.5
47	26428.0
48	27067.5
49	27707.0
50	26160.0
51	24613.0
52	22069.0
53	19525.0
54	16633.5
55	13742.0
56	12229.5
57	10717.0
58	10094.0
59	9471.0
60	8817.5
61	8164.0
62	7337.0
63	6510.0
64	5772.5
65	5035.0
66	4092.0
67	3149.0
68	2647.5
69	2146.0
70	1854.0
71	1562.0
72	1266.0
73	970.0
74	791.0
75	477.5
76	343.0
77	237.5
78	132.0
79	107.5
80	83.0
81	70.0
82	57.0
83	45.5
84	34.0
85	20.5
86	7.0
87	8.5
88	10.0
89	6.5
90	3.0
91	5.0
92	7.0
93	5.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.268959137827242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28081158124196	20.038175224279442
2	8.71946788888268	4.581026913533117
3	3.1770163769493043	2.503707401588677
4	1.6701134648706053	1.75488569456884
5	1.1089374545861048	1.456531634046427
6	0.8216421664524063	1.2950210697872464
7	0.5947124252417416	1.0935733478203415
8	0.4929852998714438	1.036016855829797
9	0.44938796042703033	1.0624458572540267
>10	5.3557654686713985	31.05701322918349
>50	0.9278408138170029	17.20821648288722
>100	0.3968475769940193	14.429353810915178
>500	0.0011178804985747023	0.2516628246729411
>1k	0.0022357609971494045	0.6216688446121544
>5k	0.0011178804985747023	1.610700809021099
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5485	1.610700809021099	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1066	0.31303683909142965	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1051	0.30863200552072473	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	857	0.2516628246729411	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	489	0.1435975744049804	No Hit
AAGGGAGGGCATGCAAAAACTAACATTGAAATTTTGAAACAGCAGCAGAGT	364	0.1068906279824394	No Hit
AAAGCAGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGC	364	0.1068906279824394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.87311142760656E-4	0.0	0.0	0.3576724859412395	0.0
2	5.87311142760656E-4	0.0	0.0	0.5056748939169249	0.0
3	5.87311142760656E-4	0.0	0.0	0.6551455797495118	0.0
4	5.87311142760656E-4	0.0	0.0	1.4030863200552073	0.0
5	5.87311142760656E-4	0.0	0.0	1.5114452258945483	0.0
6	5.87311142760656E-4	0.0	0.0	2.224440953205985	0.0
7	5.87311142760656E-4	0.0	0.0	2.7415684144067423	0.0
8	5.87311142760656E-4	0.0	0.0	3.1438765471977916	0.0
9	5.87311142760656E-4	0.0	0.0	3.780521825950343	0.0
10	5.87311142760656E-4	0.0	0.0	4.750172522648186	0.0
11	5.87311142760656E-4	0.0	0.0	5.671370050068275	0.0
12	5.87311142760656E-4	0.0	0.0	6.119782107566036	0.0
13	5.87311142760656E-4	0.0	0.0	6.296562761536993	0.0
14	5.87311142760656E-4	0.0	0.0	6.520621962500184	0.0
15	8.80966714140984E-4	0.0	0.0	6.717958506467764	0.0
16	8.80966714140984E-4	0.0	0.0	7.114980838973968	0.0
17	8.80966714140984E-4	0.0	0.0	7.546360873331669	0.0
18	8.80966714140984E-4	0.0	0.0	8.153934250517567	0.0
19	8.80966714140984E-4	0.0	0.0	8.412644808903638	0.0
20	8.80966714140984E-4	0.0	0.0	8.720983158852981	0.0
21	8.80966714140984E-4	0.0	0.0	9.046647187513765	0.0
22	8.80966714140984E-4	0.0	0.0	9.407256229168809	0.0
23	8.80966714140984E-4	0.0	0.0	9.751420558826553	0.0
24	8.80966714140984E-4	0.0	0.0	10.016297884211609	0.0
25	8.80966714140984E-4	0.0	0.0	10.257389108314857	0.0
26	8.80966714140984E-4	0.0	0.0	10.479099064707006	0.0
27	8.80966714140984E-4	0.0	0.0	10.748381223662767	0.0
28	8.80966714140984E-4	0.0	0.0	10.978607191624944	0.0
29	8.80966714140984E-4	0.0	0.0	11.238786027867913	0.0
30	8.80966714140984E-4	0.0	0.0	11.55887060067247	0.0
31	8.80966714140984E-4	0.0	0.0	11.80847783634575	0.0
32	8.80966714140984E-4	0.0	0.0	12.069537639302862	0.0
33	8.80966714140984E-4	0.0	0.0	12.315621008119576	0.0
34	8.80966714140984E-4	0.0	0.0	12.574331566505645	0.0
35	8.80966714140984E-4	0.0	0.0	12.843907381032787	0.0
36	8.80966714140984E-4	0.0	0.0	13.105554495132658	0.0
37	8.80966714140984E-4	0.0	0.0	13.364558709090108	0.0
38	8.80966714140984E-4	0.0	0.0	13.614165944763387	0.0
39	8.80966714140984E-4	0.0	0.0	13.896662604431263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	35	1.2085547E-7	45.000004	6
GATACGT	25	3.8850034E-5	45.000004	12
GGTACCC	25	3.8850034E-5	45.000004	9
TTGGACC	25	3.8850034E-5	45.000004	19
TCCGATA	25	3.8850034E-5	45.000004	11
CCGATAT	25	3.8850034E-5	45.000004	12
GTTTACC	25	3.8850034E-5	45.000004	10
ATTACGG	70	0.0	45.000004	2
TAGCGCA	25	3.8850034E-5	45.000004	40
ATTACCG	50	2.1827873E-11	45.000004	1
ATCCGAT	25	3.8850034E-5	45.000004	10
TACGCGG	35	1.2085547E-7	45.000004	2
CGCTCCG	35	1.2085547E-7	45.000004	1
CTAGTGA	25	3.8850034E-5	45.000004	30
CGAGGTT	35	1.2085547E-7	45.000004	29
GACGGGT	25	3.8850034E-5	45.000004	4
ATACGTA	25	3.8850034E-5	45.000004	13
CGCAAAC	25	3.8850034E-5	45.000004	24
TCCCACG	25	3.8850034E-5	45.000004	1
CGATAGG	25	3.8850034E-5	45.000004	2
>>END_MODULE