##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846306
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.316683327307146	34.0	31.0	34.0	30.0	34.0
2	32.49982039593244	34.0	31.0	34.0	31.0	34.0
3	32.5814303573412	34.0	31.0	34.0	31.0	34.0
4	36.10956911566266	37.0	35.0	37.0	35.0	37.0
5	35.93814175959996	37.0	35.0	37.0	35.0	37.0
6	35.91026059132276	37.0	35.0	37.0	35.0	37.0
7	35.91592993550796	37.0	35.0	37.0	35.0	37.0
8	36.03933683561265	37.0	35.0	37.0	35.0	37.0
9	37.655912873121544	39.0	38.0	39.0	35.0	39.0
10	37.32758954798855	39.0	37.0	39.0	34.0	39.0
11	37.28868281685348	39.0	37.0	39.0	34.0	39.0
12	37.21933319626707	39.0	37.0	39.0	34.0	39.0
13	37.19699494036436	39.0	37.0	39.0	34.0	39.0
14	38.46794303715205	40.0	38.0	41.0	34.0	41.0
15	38.47164500783405	40.0	38.0	41.0	34.0	41.0
16	38.472645827868405	40.0	38.0	41.0	34.0	41.0
17	38.44760287650094	40.0	38.0	41.0	34.0	41.0
18	38.37270916193434	40.0	38.0	41.0	34.0	41.0
19	38.35475229999551	40.0	38.0	41.0	34.0	41.0
20	38.27307026063859	40.0	38.0	41.0	34.0	41.0
21	38.2282909491366	40.0	37.0	41.0	34.0	41.0
22	38.16966676355833	40.0	37.0	41.0	34.0	41.0
23	38.11913657707732	40.0	37.0	41.0	33.0	41.0
24	38.03842581761207	40.0	37.0	41.0	33.0	41.0
25	37.95017523212644	40.0	37.0	41.0	33.0	41.0
26	37.86337211363266	40.0	37.0	41.0	33.0	41.0
27	37.77118914435204	40.0	37.0	41.0	33.0	41.0
28	37.694916495924645	40.0	37.0	41.0	33.0	41.0
29	37.614300264916	40.0	36.0	41.0	33.0	41.0
30	37.56990970169182	40.0	36.0	41.0	33.0	41.0
31	37.52811748941872	40.0	36.0	41.0	33.0	41.0
32	37.46795957963195	40.0	36.0	41.0	32.0	41.0
33	37.356094604079374	40.0	36.0	41.0	32.0	41.0
34	37.261000158335165	40.0	36.0	41.0	32.0	41.0
35	37.21313685593627	39.0	36.0	41.0	31.0	41.0
36	37.12341753455606	39.0	35.0	41.0	31.0	41.0
37	37.02284043832845	39.0	35.0	41.0	31.0	41.0
38	36.98231136255681	39.0	35.0	41.0	31.0	41.0
39	36.9033281106361	39.0	35.0	41.0	31.0	41.0
40	36.780304050780686	39.0	35.0	41.0	31.0	41.0
41	36.66932409790312	39.0	35.0	41.0	30.0	41.0
42	36.57892180842391	39.0	35.0	41.0	30.0	41.0
43	36.49624603866687	39.0	35.0	41.0	30.0	41.0
44	36.39911923110554	39.0	35.0	40.0	30.0	41.0
45	36.27674505438931	39.0	35.0	40.0	30.0	41.0
46	36.17610296984778	38.0	35.0	40.0	30.0	41.0
47	36.000557717894	38.0	35.0	40.0	29.0	41.0
48	35.90148835054933	38.0	35.0	40.0	29.0	41.0
49	35.730619893986336	38.0	34.0	40.0	28.0	41.0
50	35.606729717147225	38.0	34.0	40.0	28.0	41.0
51	34.49586083520618	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	5.0
11	5.0
12	8.0
13	11.0
14	20.0
15	49.0
16	61.0
17	110.0
18	216.0
19	403.0
20	695.0
21	1047.0
22	1606.0
23	2297.0
24	3116.0
25	4011.0
26	5235.0
27	6509.0
28	8134.0
29	10570.0
30	13342.0
31	17465.0
32	22708.0
33	30893.0
34	46313.0
35	59144.0
36	71902.0
37	105334.0
38	175064.0
39	259970.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.61500213870633	34.24210628307019	20.82142865582898	24.321462922394502
2	23.425687635441555	31.771723230131894	20.223536167769105	24.57905296665745
3	21.780774329852324	32.021396516153736	20.641470106557204	25.556359047436743
4	22.41943221482537	31.199471585927547	19.183604984485516	27.197491214761566
5	19.258400625778382	35.02657431236456	16.927328885769448	28.787696176087607
6	20.819065444413724	38.594905388830995	18.544474457229416	22.04155470952587
7	83.76698262803288	7.363175967085192	4.958135709778732	3.9117056951031897
8	85.36144136990639	6.258256469882053	4.59361034897543	3.786691811236125
9	75.32689121901535	12.117248371156531	7.384090388110211	5.171770021717912
10	34.0339073573861	37.53524139023001	14.522524949604518	13.908326302779372
11	27.069759637766953	29.621909805673123	25.459231058269705	17.849099498290215
12	28.79691270060711	25.71162203741909	27.1512904315933	18.3401748303805
13	24.97229134615612	27.110879516392416	26.050742875508387	21.866086261943078
14	20.37962628174679	29.24686815407193	27.115369618081402	23.258135946099873
15	20.32350001063445	30.628283386860073	28.75295696828334	20.295259634222138
16	22.60340822350308	28.743976764905366	28.513209170205577	20.139405841385976
17	21.568557944762297	29.875600551100902	27.85446398820285	20.701377515933952
18	21.791763262933266	29.62888127934813	26.92241340602572	21.656942051692887
19	22.82283240340964	29.944842645567917	26.692472935321266	20.539852015701175
20	24.702412602533837	28.234941026059136	27.57324182978734	19.4894045416197
21	23.96922626095053	29.65653085290663	26.234955205327626	20.13928768081521
22	22.78844767731766	29.488624681852666	25.903751125479435	21.81917651535024
23	22.924214173124142	29.16415575453796	26.831193445396817	21.080436626941083
24	21.632600974115746	27.9277235420758	28.572880258440804	21.866795225367657
25	21.191153081745846	31.68971979402249	25.527173386458323	21.591953737773334
26	22.319941014243074	31.32153145552554	25.819148156813256	20.539379373418125
27	22.06128752484326	30.55915945296382	25.947825018373972	21.43172800381895
28	22.24928099292691	30.505396393266736	27.35948935727739	19.88583325652896
29	21.91228704511134	29.68323514189903	26.26319558173994	22.14128223124969
30	21.186072177203044	30.81521340980685	26.514168634040168	21.484545778949933
31	22.925513939402535	30.064302982609124	25.591334576382536	21.4188485016058
32	23.119179114882797	29.94342471871876	26.58246544394108	20.35493072245736
33	22.41990485710842	31.64434613484957	25.326891219015344	20.608857789026665
34	21.339562758623952	30.38439996880561	27.505653983311003	20.77038328925944
35	23.149546381568843	28.774580352732936	27.332430586572702	20.74344267912552
36	22.83866591989186	31.82832214352728	25.716702941961888	19.616308994618965
37	21.76730402478536	31.925568293265083	26.67628493712676	19.630842744822793
38	21.11671192216527	29.20574827544647	29.283852412720695	20.393687389667566
39	22.676195135092982	28.89297724463728	27.40994392099312	21.02088369927662
40	22.90365423381141	28.542512991754755	28.48036053153351	20.073472242900323
41	19.764482350355543	29.35073129577245	30.314212589772495	20.57057376409951
42	20.740016022573396	29.564838249994686	28.211072590765042	21.48407313666688
43	21.37264771843754	29.433325534735662	27.860962819594803	21.333063927231994
44	21.86017823340494	29.073172115050582	27.232348583136595	21.83430106840788
45	20.987207936609217	29.807067420058463	27.797274271953643	21.408450371378674
46	20.16847334179363	30.83376461941662	27.535312286572466	21.46244975221728
47	20.948569429969773	29.316582890821998	28.564490857916642	21.170356821291588
48	21.758560142548912	27.783685806315916	29.463338319709422	20.99441573142575
49	20.003757506150258	30.763813561525026	27.91059025931519	21.321838673009527
50	19.730097624263564	29.175971811614236	29.00180313030984	22.092127433812355
51	20.539852015701175	28.21898934900615	28.077551145803053	23.163607489489618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4781.0
1	3773.0
2	2765.0
3	1935.5
4	1106.0
5	1104.5
6	1103.0
7	1165.0
8	1227.0
9	1480.5
10	1734.0
11	1904.5
12	2075.0
13	2155.0
14	2235.0
15	2545.0
16	2855.0
17	2908.0
18	2961.0
19	3280.0
20	3599.0
21	4205.0
22	4811.0
23	6322.0
24	7833.0
25	8572.5
26	11273.0
27	13234.0
28	16598.5
29	19963.0
30	22850.5
31	25738.0
32	29050.0
33	32362.0
34	33667.0
35	34972.0
36	36811.0
37	38650.0
38	41851.0
39	45052.0
40	45967.5
41	46883.0
42	47852.5
43	48822.0
44	51573.5
45	54325.0
46	54976.0
47	55627.0
48	55477.5
49	55328.0
50	53662.0
51	51996.0
52	47619.0
53	43242.0
54	39765.0
55	36288.0
56	35289.0
57	34290.0
58	34292.0
59	34294.0
60	33884.0
61	33474.0
62	30917.0
63	28360.0
64	24605.0
65	20850.0
66	17514.0
67	14178.0
68	12054.0
69	9930.0
70	8633.0
71	7336.0
72	6223.5
73	5111.0
74	4170.0
75	2675.0
76	2121.0
77	1584.5
78	1048.0
79	840.0
80	632.0
81	482.5
82	333.0
83	223.5
84	114.0
85	88.0
86	62.0
87	45.5
88	29.0
89	24.5
90	20.0
91	13.0
92	6.0
93	4.5
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	846306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.469354709502333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15309309537196	18.14478337757405
2	7.746246477851844	3.790913054675798
3	3.062615120524848	2.2482064716842323
4	1.8636647795164396	1.8241069819837685
5	1.3095086353186183	1.60214156463838
6	0.9716346080507466	1.4265163123453195
7	0.8180121623789199	1.401136083055577
8	0.7301313076680834	1.4292673561474492
9	0.6263593551361324	1.3793948312797049
>10	7.484858408702482	39.37777447392348
>50	0.9700283407876551	16.475372435408488
>100	0.25705751030677726	8.8810496180676
>500	0.004365127533511312	0.7491071358408528
>1k	0.002425070851950729	1.2702303033752609
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3708	0.43813939638854027	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2825	0.33380361240496936	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2038	0.24081124321462924	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1097	0.12962214612681464	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1035	0.12229619073951975	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	860	0.10161809085602606	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	847	0.1000820034361094	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1816057076282102E-4	0.0	0.0	0.10587187140348762	0.0
2	1.1816057076282102E-4	0.0	0.0	0.1534905814209045	0.0
3	1.1816057076282102E-4	0.0	0.0	0.21115293995316115	0.0
4	1.1816057076282102E-4	0.0	0.0	0.46212599225339296	0.0
5	1.1816057076282102E-4	0.0	0.0	0.5260508610360791	0.0
6	1.1816057076282102E-4	0.0	0.0	0.9511925946407092	0.0
7	1.1816057076282102E-4	0.0	0.0	1.2467121821185245	0.0
8	1.1816057076282102E-4	0.0	0.0	1.5421136090255771	0.0
9	1.1816057076282102E-4	0.0	0.0	1.891396256200476	0.0
10	1.1816057076282102E-4	0.0	0.0	2.4819627888730555	0.0
11	1.1816057076282102E-4	0.0	0.0	2.9889897980163203	0.0
12	1.1816057076282102E-4	0.0	0.0	3.228855756664847	0.0
13	1.1816057076282102E-4	0.0	0.0	3.360841114206918	0.0
14	1.1816057076282102E-4	0.0	0.0	3.496253128301111	0.0
15	1.1816057076282102E-4	0.0	0.0	3.6101599185164703	0.0
16	1.1816057076282102E-4	0.0	0.0	3.807960713973433	0.0
17	1.1816057076282102E-4	0.0	0.0	4.0325839589935555	0.0
18	1.1816057076282102E-4	0.0	0.0	4.291946411817948	0.0
19	1.1816057076282102E-4	0.0	0.0	4.426885783629089	0.0
20	1.1816057076282102E-4	0.0	0.0	4.587820481008052	0.0
21	1.1816057076282102E-4	0.0	0.0	4.768251672562879	0.0
22	1.1816057076282102E-4	0.0	0.0	4.973496583977899	0.0
23	1.1816057076282102E-4	0.0	0.0	5.165034869184432	0.0
24	1.1816057076282102E-4	0.0	0.0	5.324197158001952	0.0
25	1.1816057076282102E-4	0.0	0.0	5.464926397780472	0.0
26	1.1816057076282102E-4	0.0	0.0	5.601283696440767	0.0
27	1.1816057076282102E-4	0.0	0.0	5.745675913912935	0.0
28	1.1816057076282102E-4	0.0	0.0	5.886877795974506	0.0
29	1.1816057076282102E-4	0.0	0.0	6.04202262538609	0.0
30	1.1816057076282102E-4	0.0	0.0	6.224817028356174	0.0
31	1.1816057076282102E-4	0.0	0.0	6.375944398361822	0.0
32	1.1816057076282102E-4	0.0	0.0	6.550231240236982	0.0
33	2.3632114152564204E-4	0.0	0.0	6.698877238256611	0.0
34	3.5448171228846304E-4	0.0	0.0	6.852367819677516	0.0
35	3.5448171228846304E-4	0.0	0.0	7.012593553631901	0.0
36	3.5448171228846304E-4	0.0	0.0	7.191843139479101	0.0
37	3.5448171228846304E-4	0.0	0.0	7.36813871105723	0.0
38	3.5448171228846304E-4	0.0	0.0	7.5495151871781605	0.0
39	3.5448171228846304E-4	0.0	0.0	7.725338116473238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.03223E-4	45.000004	14
ACGTTAG	20	7.03223E-4	45.000004	1
CCGATAG	20	7.03223E-4	45.000004	1
TCGAGTT	20	7.03223E-4	45.000004	27
AATGTCG	20	7.03223E-4	45.000004	1
CATATCG	25	3.8899307E-5	45.0	1
GGTAACG	25	3.8899307E-5	45.0	1
CGAAACG	25	3.8899307E-5	45.0	1
TAAACCG	35	1.2114288E-7	45.0	1
TTATACG	35	1.2114288E-7	45.0	1
TCGTACT	35	1.2114288E-7	45.0	29
TACAGCG	60	0.0	44.999996	1
GCTCATA	30	2.1648211E-6	44.999996	31
TATACGG	130	0.0	41.53846	2
ATGGGCG	55	6.002665E-11	40.909092	5
TAACGCG	55	6.002665E-11	40.909092	1
CATACCG	50	1.0804797E-9	40.5	1
ATACGCG	45	1.9272193E-8	40.0	1
CGGTAAG	45	1.9272193E-8	40.0	1
AAACGCG	130	0.0	39.80769	1
>>END_MODULE