##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825636
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39075331017543	34.0	31.0	34.0	30.0	34.0
2	32.55976968058563	34.0	31.0	34.0	31.0	34.0
3	32.63520970500317	34.0	31.0	34.0	31.0	34.0
4	36.14067216061315	37.0	35.0	37.0	35.0	37.0
5	35.98943965621654	37.0	35.0	37.0	35.0	37.0
6	35.96233691360357	37.0	35.0	37.0	35.0	37.0
7	35.93965985010343	37.0	35.0	37.0	35.0	37.0
8	36.06737957162721	37.0	35.0	37.0	35.0	37.0
9	37.68929043791695	39.0	38.0	39.0	35.0	39.0
10	37.376564248651945	39.0	37.0	39.0	35.0	39.0
11	37.35965970476094	39.0	37.0	39.0	35.0	39.0
12	37.304911607536496	39.0	37.0	39.0	34.0	39.0
13	37.28865747133119	39.0	37.0	39.0	34.0	39.0
14	38.56843209356181	40.0	38.0	41.0	34.0	41.0
15	38.573710448672294	40.0	38.0	41.0	34.0	41.0
16	38.57524986798056	40.0	38.0	41.0	34.0	41.0
17	38.55164503485798	40.0	38.0	41.0	34.0	41.0
18	38.4759627729411	40.0	38.0	41.0	34.0	41.0
19	38.463628039475026	40.0	38.0	41.0	34.0	41.0
20	38.37584964802891	40.0	38.0	41.0	34.0	41.0
21	38.327529322849294	40.0	38.0	41.0	34.0	41.0
22	38.27490443730651	40.0	38.0	41.0	34.0	41.0
23	38.20826490123977	40.0	37.0	41.0	34.0	41.0
24	38.1597447301232	40.0	37.0	41.0	34.0	41.0
25	38.08470076401707	40.0	37.0	41.0	33.0	41.0
26	38.01568487808186	40.0	37.0	41.0	33.0	41.0
27	37.93703884036064	40.0	37.0	41.0	33.0	41.0
28	37.83361675120755	40.0	37.0	41.0	33.0	41.0
29	37.785125648590906	40.0	37.0	41.0	33.0	41.0
30	37.73236753242349	40.0	37.0	41.0	33.0	41.0
31	37.65931596974938	40.0	37.0	41.0	33.0	41.0
32	37.6067274198315	40.0	36.0	41.0	33.0	41.0
33	37.51514832202084	40.0	36.0	41.0	33.0	41.0
34	37.42111656952943	40.0	36.0	41.0	32.0	41.0
35	37.34322631280613	40.0	36.0	41.0	32.0	41.0
36	37.27997810173006	40.0	36.0	41.0	32.0	41.0
37	37.17778657907359	39.0	35.0	41.0	31.0	41.0
38	37.13260201832284	39.0	35.0	41.0	31.0	41.0
39	37.063616412075056	39.0	35.0	41.0	31.0	41.0
40	36.93339437718316	39.0	35.0	41.0	31.0	41.0
41	36.82934852646929	39.0	35.0	41.0	31.0	41.0
42	36.760339907659066	39.0	35.0	41.0	31.0	41.0
43	36.671368496528736	39.0	35.0	41.0	31.0	41.0
44	36.587098915260476	39.0	35.0	41.0	30.0	41.0
45	36.45998115392255	39.0	35.0	41.0	30.0	41.0
46	36.32582639322898	39.0	35.0	40.0	30.0	41.0
47	36.19964003507599	38.0	35.0	40.0	30.0	41.0
48	36.086224437887886	38.0	35.0	40.0	29.0	41.0
49	35.94663144533426	38.0	35.0	40.0	29.0	41.0
50	35.84178499968509	38.0	35.0	40.0	29.0	41.0
51	34.779290147231954	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	2.0
11	5.0
12	7.0
13	6.0
14	26.0
15	46.0
16	55.0
17	102.0
18	205.0
19	334.0
20	592.0
21	892.0
22	1395.0
23	2015.0
24	2930.0
25	3666.0
26	4733.0
27	5750.0
28	7327.0
29	9542.0
30	12356.0
31	15587.0
32	21058.0
33	28529.0
34	43548.0
35	56662.0
36	68145.0
37	100488.0
38	169845.0
39	269660.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.68478118686685	34.14967370608839	20.826005648978484	24.339539458066266
2	23.54306256025658	31.637670837996406	20.190132213227134	24.62913438851988
3	21.77291203387449	31.896743843533955	20.65874065568846	25.671603466903093
4	22.329816044843007	31.18250657674811	19.19986531595037	27.287812062458517
5	19.33733509682233	34.92713496020038	16.82630117872767	28.909228764249622
6	20.875179861343256	38.5404706190137	18.512031936591914	22.072317583051127
7	83.75664336341923	7.3068519299061565	4.935710167676797	4.000794538997815
8	85.28382967796946	6.256510132794597	4.579499924906375	3.8801602643295596
9	75.26016307428455	12.03762917314652	7.459340435736814	5.242867316832115
10	33.924150594208584	37.51314138433886	14.566830903691216	13.995877117761339
11	26.925545882204748	29.678332824634584	25.454922023748967	17.9411992694117
12	28.759041514662638	25.555450585972512	27.28224059997384	18.403267299391015
13	24.884331594068087	27.174687150269612	26.055307665847906	21.8856735898144
14	20.39518625641324	29.122034407414404	27.266616281266803	23.21616305490555
15	20.38258990644788	30.487284953659966	28.79452930831504	20.33559583157711
16	22.534264494280773	28.635621508752042	28.513170452838782	20.316943544128403
17	21.58554132813976	29.795939130561166	27.88177841082511	20.736741130473963
18	21.81590919000625	29.55999980621	27.073189638048728	21.55090136573502
19	22.820225862244378	29.89586209903638	26.70099172032227	20.58292031839697
20	24.690178238352008	28.173311241273396	27.438604905793834	19.697905614580762
21	23.952686171630113	29.673488074647906	26.281678608975383	20.092147144746594
22	22.864555324622472	29.50125721262154	25.846377822672462	21.787809640083523
23	22.90609905575823	29.149165007339796	26.809756357523167	21.134979579378808
24	21.56870582193606	27.902489717018153	28.681888871124805	21.846915589920982
25	21.268331322762087	31.667223812915136	25.480599198678348	21.583845665644425
26	22.390981013424803	31.29890169517802	25.89131287879889	20.418804412598288
27	22.05063732686075	30.644254853228297	25.891070641299557	21.414037178611398
28	22.289967976202586	30.463666797474914	27.34788696229331	19.898478264029183
29	21.996133889510634	29.5263287938026	26.37760465871159	22.099932657975184
30	21.209952085422632	30.729522452993812	26.547292026994946	21.513233434588606
31	22.929838330692945	30.13398156088155	25.632118754511673	21.30406135391383
32	23.14615641759807	29.858678642888638	26.616329714305092	20.378835225208203
33	22.361791394755073	31.614658275559687	25.40066082389818	20.622889505787054
34	21.418881928598076	30.30996710414759	27.536347736775046	20.73480323047929
35	23.078693274033593	28.733243220983578	27.351883881032318	20.836179623950507
36	22.942434680658305	31.663711369174795	25.7550542854236	19.6387996647433
37	21.781026990102177	32.031306774413906	26.584717720642026	19.60294851484189
38	21.1002184982244	29.23673386334898	29.312917556889477	20.350130081537145
39	22.618442025299284	28.88960752680358	27.455198174498207	21.036752273398932
40	22.889263549554524	28.510384721596445	28.50699339660577	20.093358332243263
41	19.73024432074183	29.372023506726936	30.276174972990518	20.621557199540717
42	20.741343642961304	29.482120450174172	28.179730535005742	21.596805371858785
43	21.428450309821763	29.386436637937297	27.963291329350948	21.22182172288999
44	21.897300989782423	28.960583114108395	27.211143893919353	21.930972002189826
45	20.990848267275165	29.84789907416828	27.732075636236793	21.429177022319763
46	20.127634938399	30.847855471418395	27.654680755199628	21.36982883498297
47	20.997509798506847	29.355188000523235	28.470778890455357	21.17652331051456
48	21.783933840094182	27.692590923845373	29.529235643794603	20.99423959226584
49	20.099414269726612	30.597987490855534	27.910241316996835	21.39235692242102
50	19.794316139315633	29.068863276310626	28.917222601727637	22.219597982646107
51	20.639482774491423	28.23786753484586	27.941368835661233	23.181280855001475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4637.0
1	3688.5
2	2740.0
3	1872.0
4	1004.0
5	1056.5
6	1109.0
7	1174.5
8	1240.0
9	1485.0
10	1730.0
11	1862.0
12	1994.0
13	2091.5
14	2189.0
15	2429.0
16	2669.0
17	2763.0
18	2857.0
19	3153.5
20	3450.0
21	4027.0
22	4604.0
23	6254.0
24	7904.0
25	8530.0
26	10929.0
27	12702.0
28	15917.0
29	19132.0
30	22058.0
31	24984.0
32	28027.5
33	31071.0
34	32481.5
35	33892.0
36	35756.5
37	37621.0
38	41004.0
39	44387.0
40	45065.5
41	45744.0
42	46750.0
43	47756.0
44	50271.0
45	52786.0
46	53609.0
47	54432.0
48	54059.0
49	53686.0
50	52200.0
51	50714.0
52	46709.0
53	42704.0
54	39210.5
55	35717.0
56	34482.0
57	33247.0
58	33395.0
59	33543.0
60	33369.0
61	33195.0
62	30484.0
63	27773.0
64	23957.0
65	20141.0
66	17007.0
67	13873.0
68	11808.5
69	9744.0
70	8432.5
71	7121.0
72	6062.5
73	5004.0
74	4118.5
75	2632.0
76	2031.0
77	1541.5
78	1052.0
79	810.0
80	568.0
81	421.0
82	274.0
83	198.5
84	123.0
85	92.0
86	61.0
87	38.5
88	16.0
89	15.0
90	14.0
91	10.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	825636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.12142824383076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.23402142796198	17.66509192881749
2	8.102232864042852	3.9087485728963407
3	3.1745678045047034	2.2972532850460667
4	1.8297371862203946	1.7654349696993619
5	1.3688805158509831	1.6509676568738763
6	1.036687198464846	1.5003825521440632
7	0.8551896453333563	1.4439876965342942
8	0.7602919523325915	1.467146221804208
9	0.6937724905773001	1.5061305014103685
>10	7.690171483428064	39.73756185012542
>50	0.999322672348815	16.62995817145362
>100	0.24806597113754109	8.415984521414236
>500	0.005041991283283355	0.8670292006121778
>1k	0.0020167965133133423	1.144322871168464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3613	0.4376020425465944	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2827	0.34240270530839256	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1910	0.23133681186382377	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1059	0.12826475589727193	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	984	0.11918084967225265	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	851	0.10307205596655185	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10416212471355416	0.0
2	0.0	0.0	0.0	0.15939227456167124	0.0
3	0.0	0.0	0.0	0.21789263065079525	0.0
4	0.0	0.0	0.0	0.4721208862016676	0.0
5	0.0	0.0	0.0	0.5357082297768023	0.0
6	0.0	0.0	0.0	0.9574437161170298	0.0
7	0.0	0.0	0.0	1.2578182152909998	0.0
8	0.0	0.0	0.0	1.5653387206953184	0.0
9	0.0	0.0	0.0	1.920458894718738	0.0
10	0.0	0.0	0.0	2.530897393040032	0.0
11	0.0	0.0	0.0	3.060549685333488	0.0
12	0.0	0.0	0.0	3.3141723471360263	0.0
13	0.0	0.0	0.0	3.4431638155312996	0.0
14	0.0	0.0	0.0	3.5892330276296094	0.0
15	0.0	0.0	0.0	3.7023579398185156	0.0
16	0.0	0.0	0.0	3.9062007955079476	0.0
17	0.0	0.0	0.0	4.13681089487377	0.0
18	0.0	0.0	0.0	4.40097088789733	0.0
19	0.0	0.0	0.0	4.534201512530946	0.0
20	0.0	0.0	0.0	4.6927459558449485	0.0
21	0.0	0.0	0.0	4.878420999084343	0.0
22	0.0	0.0	0.0	5.077419104787097	0.0
23	0.0	0.0	0.0	5.269150085509837	0.0
24	0.0	0.0	0.0	5.42611998507817	0.0
25	0.0	0.0	0.0	5.577155065912824	0.0
26	0.0	0.0	0.0	5.722255328013798	0.0
27	0.0	0.0	0.0	5.87450159634512	0.0
28	0.0	0.0	0.0	6.015483820957419	0.0
29	0.0	0.0	0.0	6.180205320504435	0.0
30	0.0	0.0	0.0	6.374358676220513	0.0
31	0.0	0.0	0.0	6.529754032043177	0.0
32	0.0	0.0	0.0	6.70125818157154	0.0
33	0.0	0.0	0.0	6.859318149886875	1.2111874966692344E-4
34	0.0	0.0	0.0	7.019437136946548	1.2111874966692344E-4
35	0.0	0.0	0.0	7.184885348991565	1.2111874966692344E-4
36	0.0	0.0	0.0	7.369470323483957	1.2111874966692344E-4
37	0.0	0.0	0.0	7.541822304259989	1.2111874966692344E-4
38	0.0	0.0	0.0	7.730404197491389	1.2111874966692344E-4
39	0.0	0.0	0.0	7.906510859507095	1.2111874966692344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAC	20	7.032129E-4	45.000004	40
CGTATAG	20	7.032129E-4	45.000004	1
ATTCCCG	20	7.032129E-4	45.000004	1
GTAGCGT	20	7.032129E-4	45.000004	26
CGTAGCG	20	7.032129E-4	45.000004	1
TGCGACG	20	7.032129E-4	45.000004	1
GCGATAG	20	7.032129E-4	45.000004	1
TCGTACC	20	7.032129E-4	45.000004	41
GTATACG	40	6.8102963E-9	45.000004	1
CGTAAAG	20	7.032129E-4	45.000004	1
CGGAGTT	20	7.032129E-4	45.000004	23
AAATGCG	30	2.1647556E-6	45.000004	1
CCCGACG	20	7.032129E-4	45.000004	1
TATCACG	25	3.8898484E-5	45.0	1
CATATCG	25	3.8898484E-5	45.0	1
ATCGTAC	35	1.2113742E-7	45.0	28
TAGCACG	35	1.2113742E-7	45.0	1
ATATCCG	25	3.8898484E-5	45.0	1
CGATAAG	25	3.8898484E-5	45.0	1
ACGGGTC	70	0.0	41.785713	5
>>END_MODULE