FastQCFastQC Report
Sat 18 Jun 2016
SRR3554286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588347
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29030.49341630024458355No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23050.3917756018132157No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10600.1801657865171404No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT7020.11931734163682316No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT6690.1137084067735537No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6630.11268860043477744No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5900.10028095664633287No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG253.888475E-545.0000041
TAACGCG253.888475E-545.0000041
CCTCGAG253.888475E-545.0000041
ATAGTCG302.163688E-645.0000042
TAAGCGA253.888475E-545.00000427
GATTACG302.163688E-645.00000439
ACGCGAT406.8048394E-945.025
CCTTACG406.8048394E-945.01
CTCGTAG207.0304755E-445.020
CCGATGT207.0304755E-445.011
TAAACCG453.8380676E-1045.01
CGCGATT406.8048394E-945.026
TCGATAG406.8048394E-945.01
TACGCGA406.8048394E-945.024
ACTAACG351.2105738E-745.01
CGTACGG207.0304755E-445.02
CGACTTA207.0304755E-445.01
GACGAAA207.0304755E-445.023
CGGTAAT207.0304755E-445.06
TAATGCG351.2105738E-745.01