##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588347
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29629113431359	34.0	31.0	34.0	30.0	34.0
2	32.481027352905684	34.0	31.0	34.0	30.0	34.0
3	32.587037921498705	34.0	31.0	34.0	31.0	34.0
4	36.10225768126633	37.0	35.0	37.0	35.0	37.0
5	35.93351202606625	37.0	35.0	37.0	35.0	37.0
6	35.907088843828554	37.0	35.0	37.0	35.0	37.0
7	35.94868844406447	37.0	35.0	37.0	35.0	37.0
8	36.07393425988405	37.0	35.0	37.0	35.0	37.0
9	37.702245443590265	39.0	38.0	39.0	35.0	39.0
10	37.31525953221483	39.0	37.0	39.0	34.0	39.0
11	37.27405085774212	39.0	37.0	39.0	34.0	39.0
12	37.22283618340877	39.0	37.0	39.0	34.0	39.0
13	37.183495454213244	39.0	37.0	39.0	33.0	39.0
14	38.44936066640945	40.0	38.0	41.0	34.0	41.0
15	38.45057933498428	40.0	38.0	41.0	34.0	41.0
16	38.44844624005901	40.0	38.0	41.0	34.0	41.0
17	38.41933076908695	40.0	38.0	41.0	34.0	41.0
18	38.339707689509765	40.0	38.0	41.0	34.0	41.0
19	38.322201014027435	40.0	38.0	41.0	34.0	41.0
20	38.252378273365885	40.0	37.0	41.0	34.0	41.0
21	38.22112970746855	40.0	37.0	41.0	34.0	41.0
22	38.14675183182714	40.0	37.0	41.0	33.0	41.0
23	38.07970806343875	40.0	37.0	41.0	33.0	41.0
24	38.019988204240015	40.0	37.0	41.0	33.0	41.0
25	37.946664128482	40.0	37.0	41.0	33.0	41.0
26	37.84144390980153	40.0	37.0	41.0	33.0	41.0
27	37.753735465635074	40.0	37.0	41.0	33.0	41.0
28	37.69215276019084	40.0	37.0	41.0	33.0	41.0
29	37.58703112278978	40.0	36.0	41.0	33.0	41.0
30	37.56789105748818	40.0	36.0	41.0	33.0	41.0
31	37.50488062316966	40.0	36.0	41.0	33.0	41.0
32	37.43828726924757	40.0	36.0	41.0	32.0	41.0
33	37.34269402240515	40.0	36.0	41.0	32.0	41.0
34	37.23207562883808	39.0	36.0	41.0	32.0	41.0
35	37.200339255575365	39.0	36.0	41.0	32.0	41.0
36	37.06752647672207	39.0	35.0	41.0	31.0	41.0
37	37.00542027069059	39.0	35.0	41.0	31.0	41.0
38	36.97073495743158	39.0	35.0	41.0	31.0	41.0
39	36.88843148686065	39.0	35.0	41.0	31.0	41.0
40	36.81431706119008	39.0	35.0	41.0	31.0	41.0
41	36.73018473791827	39.0	35.0	41.0	31.0	41.0
42	36.6338827256704	39.0	35.0	41.0	30.0	41.0
43	36.55278602593368	39.0	35.0	41.0	30.0	41.0
44	36.45454808131936	39.0	35.0	40.0	30.0	41.0
45	36.31799431287998	39.0	35.0	40.0	30.0	41.0
46	36.22460044837485	38.0	35.0	40.0	30.0	41.0
47	36.07537728585341	38.0	35.0	40.0	29.0	41.0
48	35.974358669288705	38.0	35.0	40.0	29.0	41.0
49	35.84091361050537	38.0	35.0	40.0	29.0	41.0
50	35.71040389430047	38.0	34.0	40.0	28.0	41.0
51	34.619770305618964	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	6.0
13	9.0
14	11.0
15	28.0
16	49.0
17	91.0
18	149.0
19	266.0
20	485.0
21	725.0
22	1085.0
23	1538.0
24	2200.0
25	2733.0
26	3590.0
27	4354.0
28	5467.0
29	7039.0
30	9091.0
31	12065.0
32	15594.0
33	21423.0
34	32827.0
35	42506.0
36	50488.0
37	73447.0
38	121663.0
39	179346.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.19377510210811	34.521634341638524	21.45026659437373	24.83432396187964
2	22.109401424669457	32.917818906189716	20.564734756869672	24.40804491227116
3	21.14466462818711	31.604308341845883	21.37905011838252	25.871976911584486
4	21.601367900235747	31.463575067094755	19.971377435425016	26.963679597244482
5	19.350825278279654	34.30968459089619	17.735452037658046	28.604038093166107
6	20.634591491075845	38.453667648513544	19.117119658976762	21.79462120143385
7	84.8087948098656	6.94335145755821	4.455023990944119	3.7928297416320635
8	86.48416665675188	6.077875811383418	3.9792843339049915	3.4586731979597074
9	76.17426450717008	12.095753016502165	6.982104098431707	4.747878377896037
10	32.96914915857479	40.80058196948399	13.204792409921357	13.025476462019864
11	25.106612254332898	28.711287726460743	28.564265645953835	17.61783437325252
12	28.528912359542925	24.889903407342945	28.83298461622138	17.74819961689275
13	24.211222288887342	27.01653955913772	27.419023127508087	21.353215024466856
14	20.139815449046225	29.505206961198066	27.70847815999742	22.646499429758286
15	20.282418368751774	31.0993342364285	28.57616338657289	20.04208400824683
16	22.874766081921045	29.193656124701917	27.976177323926187	19.95540046945085
17	21.39026798810906	30.418953440741603	27.424971997817615	20.765806573331723
18	21.698419470142618	30.535721266531485	26.616435538891164	21.14942372443473
19	22.384069265246527	31.306014987753827	26.24097683849837	20.068938908501273
20	24.553537283269907	28.834174390283284	27.741791833730773	18.870496492716033
21	23.426651278922133	29.413424390708204	27.187697056329004	19.97222727404066
22	21.786462750723636	29.873696984942562	27.249395339824968	21.090444924508837
23	22.659077041269864	29.496538607318467	27.71884619110831	20.125538160303357
24	21.055091638097924	28.608627221690604	29.34526733373332	20.99101380647815
25	20.37063161705592	32.40298667283083	25.85243062342461	21.37395108668864
26	22.59805862866642	32.201404953199386	25.340657809082057	19.859878609052142
27	21.02942651190539	31.175479776390464	26.24709567653103	21.547998035173123
28	21.306643868329402	31.267432314603454	27.846152015732212	19.57977180133493
29	20.628982556212573	31.18006890491496	26.472642845123712	21.718305693748757
30	21.142794983232687	31.208623482400693	26.81172845276682	20.836853081599806
31	20.775494733550097	31.405786041230773	26.26749180330655	21.55122742191258
32	23.065809802718466	30.336859030470116	26.45411636330261	20.143214803508812
33	21.563125162531634	31.693711364211936	25.57657300878563	21.166590464470797
34	20.543658759201627	31.737053133609926	26.98050640183429	20.738781705354153
35	22.52225302415071	30.347227061581005	26.885834380051225	20.24468553421705
36	21.46046465776149	33.60465847535553	25.746540731915008	19.18833613496797
37	20.58462098047581	33.342908181736284	26.80968884008927	19.262781997698635
38	21.064099927423783	30.581782519499544	28.282459161005324	20.071658392071345
39	21.632471993568423	30.581442584053285	26.649069341732005	21.137016080646283
40	22.47942115792211	29.77596554414317	27.695560613039582	20.049052684895138
41	19.58487083302881	30.184567950546192	29.253654730966588	20.97690648545841
42	20.317431719716424	30.544899523580472	26.76158797444365	22.37608078225945
43	21.139735564216355	30.52603310631311	27.780034571434886	20.554196758035648
44	22.481120835153405	29.640841204255313	25.92398703486208	21.954050925729206
45	20.9473321016339	30.794922044303785	26.551337900932616	21.7064079531297
46	18.914178197560283	32.228769756623215	27.334719136835915	21.522332908980584
47	20.134376481906084	31.15151432742922	28.31373322206113	20.400375968603562
48	20.76461679926982	29.2346183459761	28.620864897755915	21.379899956998166
49	19.71302649626836	31.461195518970946	27.237497599205913	21.588280385554786
50	19.53727987055258	29.719536260064217	29.16102232186108	21.582161547522123
51	19.92531618245695	28.90794038212143	28.054702412011963	23.112041023409656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3616.0
1	2799.5
2	1983.0
3	1363.0
4	743.0
5	770.0
6	797.0
7	941.0
8	1085.0
9	1271.0
10	1457.0
11	1620.5
12	1784.0
13	2061.5
14	2339.0
15	2410.5
16	2482.0
17	2475.0
18	2468.0
19	2389.5
20	2311.0
21	2695.5
22	3080.0
23	4875.5
24	6671.0
25	6383.0
26	8019.0
27	9943.0
28	11657.0
29	13371.0
30	16328.5
31	19286.0
32	21110.5
33	22935.0
34	23666.0
35	24397.0
36	26412.0
37	28427.0
38	29525.5
39	30624.0
40	32012.0
41	33400.0
42	34444.0
43	35488.0
44	36060.5
45	36633.0
46	38976.0
47	41319.0
48	40748.0
49	40177.0
50	38531.5
51	36886.0
52	34172.0
53	31458.0
54	29369.5
55	27281.0
56	25198.0
57	23115.0
58	22681.0
59	22247.0
60	20997.0
61	19747.0
62	18904.0
63	18061.0
64	15331.5
65	12602.0
66	10457.5
67	8313.0
68	7015.5
69	5718.0
70	4628.5
71	3539.0
72	2941.0
73	2343.0
74	2067.0
75	1471.0
76	1151.0
77	866.0
78	581.0
79	456.5
80	332.0
81	241.5
82	151.0
83	98.5
84	46.0
85	40.0
86	34.0
87	25.5
88	17.0
89	14.0
90	11.0
91	7.0
92	3.0
93	4.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.19707307606075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60982844668158	18.54752226487331
2	8.210301204338935	4.137511188443954
3	3.4356878916650233	2.5970783661846206
4	1.9949596343673257	2.010685747637798
5	1.318487555029388	1.6611013636976077
6	1.0501065703286345	1.587576719413328
7	0.9029668630700927	1.5926485423826926
8	0.7159274737008283	1.4431421497599466
9	0.6355312192683523	1.4412173916620394
>10	6.957296573053465	37.51093341581734
>50	0.863119525345636	15.025313385195215
>100	0.299006842637412	10.644261452257421
>500	0.0047461403593240005	0.7301753221562991
>1k	0.002034060153996	1.0708326905184096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2903	0.49341630024458355	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2305	0.3917756018132157	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1060	0.1801657865171404	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	702	0.11931734163682316	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	669	0.1137084067735537	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	663	0.11268860043477744	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	590	0.10028095664633287	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6996772312937773E-4	0.0	0.0	0.1558604021096394	0.0
2	1.6996772312937773E-4	0.0	0.0	0.22231778185322607	0.0
3	1.6996772312937773E-4	0.0	0.0	0.29965309587709293	0.0
4	1.6996772312937773E-4	0.0	0.0	0.6621942493120556	0.0
5	1.6996772312937773E-4	0.0	0.0	0.721343016961079	0.0
6	1.6996772312937773E-4	0.0	0.0	1.1049601680640846	0.0
7	1.6996772312937773E-4	0.0	0.0	1.364330913559515	0.0
8	1.6996772312937773E-4	0.0	0.0	1.6332198515501906	0.0
9	1.6996772312937773E-4	0.0	0.0	2.0017098752946816	0.0
10	1.6996772312937773E-4	0.0	0.0	2.5911579391073634	0.0
11	1.6996772312937773E-4	0.0	0.0	3.0850841425213353	0.0
12	1.6996772312937773E-4	0.0	0.0	3.316240245977289	0.0
13	1.6996772312937773E-4	0.0	0.0	3.4306285236433602	0.0
14	1.6996772312937773E-4	0.0	0.0	3.5550448969740645	0.0
15	1.6996772312937773E-4	0.0	0.0	3.6616146593761845	0.0
16	1.6996772312937773E-4	0.0	0.0	3.867445572085861	0.0
17	1.6996772312937773E-4	0.0	0.0	4.1242668017343505	0.0
18	1.6996772312937773E-4	0.0	0.0	4.417801059578786	0.0
19	1.6996772312937773E-4	0.0	0.0	4.563803333746922	0.0
20	1.6996772312937773E-4	0.0	0.0	4.737170411338886	0.0
21	1.6996772312937773E-4	0.0	0.0	4.912747069331534	0.0
22	1.6996772312937773E-4	0.0	0.0	5.144243108233747	0.0
23	1.6996772312937773E-4	0.0	0.0	5.355343020360434	0.0
24	1.6996772312937773E-4	0.0	0.0	5.5271803884442345	0.0
25	1.6996772312937773E-4	0.0	0.0	5.678451662029381	0.0
26	1.6996772312937773E-4	0.0	0.0	5.811196453793425	0.0
27	1.6996772312937773E-4	0.0	0.0	5.975555242059533	0.0
28	1.6996772312937773E-4	0.0	0.0	6.134984966354889	0.0
29	1.6996772312937773E-4	0.0	0.0	6.29322491658834	0.0
30	1.6996772312937773E-4	0.0	0.0	6.5044947964381565	0.0
31	1.6996772312937773E-4	0.0	0.0	6.66749384291923	0.0
32	1.6996772312937773E-4	0.0	0.0	6.85326856429964	0.0
33	1.6996772312937773E-4	0.0	0.0	7.023236287429017	0.0
34	1.6996772312937773E-4	0.0	0.0	7.211900460102626	1.6996772312937773E-4
35	1.6996772312937773E-4	0.0	0.0	7.402944180900047	1.6996772312937773E-4
36	1.6996772312937773E-4	0.0	0.0	7.604185965085231	1.6996772312937773E-4
37	1.6996772312937773E-4	0.0	0.0	7.803218168869732	1.6996772312937773E-4
38	1.6996772312937773E-4	0.0	0.0	8.008029275240633	1.6996772312937773E-4
39	1.6996772312937773E-4	0.0	0.0	8.207571382194521	1.6996772312937773E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	25	3.888475E-5	45.000004	1
TAACGCG	25	3.888475E-5	45.000004	1
CCTCGAG	25	3.888475E-5	45.000004	1
ATAGTCG	30	2.163688E-6	45.000004	2
TAAGCGA	25	3.888475E-5	45.000004	27
GATTACG	30	2.163688E-6	45.000004	39
ACGCGAT	40	6.8048394E-9	45.0	25
CCTTACG	40	6.8048394E-9	45.0	1
CTCGTAG	20	7.0304755E-4	45.0	20
CCGATGT	20	7.0304755E-4	45.0	11
TAAACCG	45	3.8380676E-10	45.0	1
CGCGATT	40	6.8048394E-9	45.0	26
TCGATAG	40	6.8048394E-9	45.0	1
TACGCGA	40	6.8048394E-9	45.0	24
ACTAACG	35	1.2105738E-7	45.0	1
CGTACGG	20	7.0304755E-4	45.0	2
CGACTTA	20	7.0304755E-4	45.0	1
GACGAAA	20	7.0304755E-4	45.0	23
CGGTAAT	20	7.0304755E-4	45.0	6
TAATGCG	35	1.2105738E-7	45.0	1
>>END_MODULE