##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31596430996237	34.0	31.0	34.0	30.0	34.0
2	32.48854724724367	34.0	31.0	34.0	30.0	34.0
3	32.58997138529793	34.0	31.0	34.0	31.0	34.0
4	36.10117812308445	37.0	35.0	37.0	35.0	37.0
5	35.93049733506689	37.0	35.0	37.0	35.0	37.0
6	35.899456842927	37.0	35.0	37.0	35.0	37.0
7	35.90151429773034	37.0	35.0	37.0	35.0	37.0
8	36.029418717478606	37.0	35.0	37.0	35.0	37.0
9	37.64710320809018	39.0	37.0	39.0	35.0	39.0
10	37.28245267304198	39.0	37.0	39.0	34.0	39.0
11	37.24828820310116	39.0	37.0	39.0	34.0	39.0
12	37.17646832454185	39.0	37.0	39.0	34.0	39.0
13	37.148404750975125	39.0	37.0	39.0	33.0	39.0
14	38.428389028007224	40.0	38.0	41.0	34.0	41.0
15	38.4438604833988	40.0	38.0	41.0	34.0	41.0
16	38.447380284167906	40.0	38.0	41.0	34.0	41.0
17	38.3969623335963	40.0	38.0	41.0	34.0	41.0
18	38.335146605680606	40.0	38.0	41.0	34.0	41.0
19	38.337953100475815	40.0	38.0	41.0	34.0	41.0
20	38.25817553099376	40.0	38.0	41.0	34.0	41.0
21	38.21019711434061	40.0	37.0	41.0	34.0	41.0
22	38.12648467972699	40.0	37.0	41.0	33.0	41.0
23	38.088713822742776	40.0	37.0	41.0	33.0	41.0
24	37.9970601898301	40.0	37.0	41.0	33.0	41.0
25	37.940463002921945	40.0	37.0	41.0	33.0	41.0
26	37.8237873111251	40.0	37.0	41.0	33.0	41.0
27	37.75385446359116	40.0	37.0	41.0	33.0	41.0
28	37.67805711395578	40.0	37.0	41.0	33.0	41.0
29	37.59937492956275	40.0	36.0	41.0	33.0	41.0
30	37.56750155992732	40.0	36.0	41.0	33.0	41.0
31	37.49688701728152	40.0	36.0	41.0	32.0	41.0
32	37.452662046899526	40.0	36.0	41.0	32.0	41.0
33	37.35962853113945	40.0	36.0	41.0	32.0	41.0
34	37.24799408464564	40.0	36.0	41.0	32.0	41.0
35	37.21701818861514	40.0	36.0	41.0	31.0	41.0
36	37.08097603346903	39.0	35.0	41.0	31.0	41.0
37	37.01790136861289	39.0	35.0	41.0	31.0	41.0
38	36.98078884219033	39.0	35.0	41.0	31.0	41.0
39	36.89398953817905	39.0	35.0	41.0	31.0	41.0
40	36.79688784191271	39.0	35.0	41.0	31.0	41.0
41	36.710705636903896	39.0	35.0	41.0	31.0	41.0
42	36.62251408057746	39.0	35.0	41.0	30.0	41.0
43	36.54618346944329	39.0	35.0	41.0	30.0	41.0
44	36.45512357098288	39.0	35.0	41.0	30.0	41.0
45	36.33551631532797	39.0	35.0	40.0	30.0	41.0
46	36.21675568102166	39.0	35.0	40.0	30.0	41.0
47	36.08082622547066	38.0	35.0	40.0	29.0	41.0
48	35.973984260539474	38.0	35.0	40.0	29.0	41.0
49	35.7833927525914	38.0	35.0	40.0	28.0	41.0
50	35.67962666197543	38.0	34.0	40.0	28.0	41.0
51	34.643654875356994	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	6.0
12	12.0
13	13.0
14	22.0
15	19.0
16	79.0
17	123.0
18	215.0
19	372.0
20	625.0
21	1022.0
22	1522.0
23	2069.0
24	2818.0
25	3508.0
26	4504.0
27	5680.0
28	7203.0
29	9027.0
30	11454.0
31	15056.0
32	19563.0
33	26050.0
34	40051.0
35	50744.0
36	60041.0
37	87922.0
38	149763.0
39	228018.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.873617024785666	32.7360712921146	19.386941690329003	26.00336999277073
2	22.287581879004613	32.521804622882414	19.956349522675985	25.23426397543699
3	22.868259670862205	31.56193392505202	19.880620892305917	25.689185511779854
4	22.464327829378313	31.530460501540684	18.972701959048816	27.032509710032187
5	19.819185869120037	34.694020599286965	17.29650163964167	28.190291891951325
6	20.60368500188291	38.07693259189827	19.384330358247272	21.935052047971546
7	83.93453527909642	7.225006116014613	4.814746604581101	4.025712000307862
8	85.45391823506937	6.2156575471620314	4.552376449632901	3.778047768135701
9	75.55944353887723	11.663308585235253	7.68638726329649	5.090860612591019
10	33.1776612909876	37.026215025329925	15.329481389448569	14.466642294233903
11	26.74526318104228	29.387106616565738	26.662250308549503	17.20537989384248
12	30.380374877336113	24.473404269940268	27.425858784658562	17.720362068065057
13	24.353008117119618	26.8798155025165	26.543915733688106	22.223260646675776
14	19.718305987647025	28.59367397931275	27.06604471150278	24.62197532153744
15	20.39876415273269	30.951706849111737	28.33721368118109	20.312315316974484
16	22.16196306202051	28.212007179788838	28.023029200190212	21.603000558000435
17	20.99029958850904	30.44442123260372	27.63270377323742	20.93257540564982
18	20.85657189821852	30.8978309451098	27.059035346441302	21.186561810230376
19	23.131729334055343	29.534990475509826	26.963240690601133	20.3700394998337
20	24.773020266685723	28.229324434646603	27.571131311520926	19.426523987146748
21	23.48797000541508	29.98977457332208	25.910324107542905	20.61193131371994
22	22.26435476733031	30.23482747341252	26.39190871882551	21.108909040431666
23	23.30064128818386	29.34422579501318	27.455270630210638	19.89986228659232
24	21.145605128106453	28.020005552516636	29.80327048727457	21.03111883210234
25	20.728754064744543	32.367323714468704	25.85864722003084	21.04527500075591
26	22.16017636112249	31.220124299407093	26.18973664028763	20.42996269918279
27	21.505968955384706	31.087083801769662	26.363184065926514	21.04376317691912
28	21.593654737918467	30.58337158705769	27.658267339932213	20.16470633509163
29	21.199206155047154	29.692632470127734	25.384484289401566	23.723677085423546
30	20.82289945821731	31.054510870013385	26.69537299442824	21.42721667734106
31	21.266963350641426	31.49321465974343	25.719696865576868	21.520125124038277
32	22.88846313486293	29.58281908416461	26.455680197031878	21.073037583940582
33	20.97133307128387	32.121583621725186	25.75969147798647	21.147391829004476
34	20.642305228986334	30.245135363208803	27.826766978468882	21.285792429335977
35	24.18162226943999	28.429984689347688	26.767940538594114	20.620452502618203
36	21.235764804191327	33.47631521801874	25.428189742137832	19.859730235652105
37	20.610144612821916	32.96408181440851	27.579927377480423	18.845846195289155
38	20.297059640075975	29.1395798229242	30.42435520713361	20.139005329866215
39	20.82592310589089	30.5202873015044	28.501727602329858	20.15206199027485
40	22.09667975997735	30.258054585086818	28.929711186671764	18.71555446826407
41	18.814372771777823	29.858245899521442	31.192636593283652	20.134744735417083
42	19.579218194662438	30.28100681969989	27.52165344049874	22.618121545138937
43	21.1531642472904	30.53018287570884	28.058763218150684	20.25788965885008
44	21.320426939051508	29.852336042704902	26.675306968958136	22.151930049285458
45	20.55049629053406	30.335982231946762	27.413489316903018	21.700032160616164
46	18.97586304525301	32.08103925519312	27.292543409959897	21.65055428959398
47	20.543899241064434	29.801758663437777	28.838864317933805	20.815477777563984
48	20.532629281553824	28.39768663465265	29.949230206790013	21.12045387700351
49	18.807500845246963	32.62818204558011	27.201009348568856	21.36330776060407
50	19.094747374236874	30.33323346133442	29.533616090203658	21.038403074225055
51	19.73163752511689	28.925725469283865	28.382843273345998	22.95979373225325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3977.0
1	3186.5
2	2396.0
3	1655.0
4	914.0
5	944.5
6	975.0
7	1036.5
8	1098.0
9	1185.5
10	1273.0
11	1383.0
12	1493.0
13	2013.0
14	2533.0
15	2487.5
16	2442.0
17	2332.5
18	2223.0
19	2337.0
20	2451.0
21	3287.0
22	4123.0
23	6031.0
24	7939.0
25	7647.5
26	9347.0
27	11338.0
28	14926.0
29	18514.0
30	20018.5
31	21523.0
32	24297.0
33	27071.0
34	31528.0
35	35985.0
36	37005.5
37	38026.0
38	38970.0
39	39914.0
40	41277.5
41	42641.0
42	44138.5
43	45636.0
44	45917.0
45	46198.0
46	46861.5
47	47525.0
48	46857.0
49	46189.0
50	43315.0
51	40441.0
52	38503.5
53	36566.0
54	33113.0
55	29660.0
56	28688.5
57	27717.0
58	27357.5
59	26998.0
60	27676.0
61	28354.0
62	26049.0
63	23744.0
64	20781.5
65	17819.0
66	14550.5
67	11282.0
68	9484.5
69	7687.0
70	6803.0
71	5919.0
72	4828.0
73	3737.0
74	3138.5
75	2115.0
76	1690.0
77	1263.5
78	837.0
79	637.0
80	437.0
81	323.5
82	210.0
83	156.5
84	103.0
85	76.0
86	49.0
87	37.0
88	25.0
89	16.5
90	8.0
91	8.5
92	9.0
93	8.0
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	727598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.105939069158776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49542313452592	16.750748297392395
2	7.726982206955381	3.5707836012477006
3	3.242975684629824	2.247959957154605
4	1.99044620359973	1.8396451480325506
5	1.4703525376489617	1.6986938072549944
6	1.1070076130649875	1.5347070273944696
7	0.9601700502002652	1.5529941473170914
8	0.8581446693008045	1.5862590753110237
9	0.7443989076795183	1.5480032223592166
>10	8.04309125012767	38.96220920164205
>50	1.05982565059937	16.87283095407423
>100	0.2927992899113513	9.749235616457973
>500	0.005987715540109434	0.9544890021988103
>1k	0.0023950862160437734	1.1314409421629035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2914	0.4004958782184668	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2666	0.36641112262540576	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1538	0.2113804600892251	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1060	0.14568484245421237	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	919	0.12630600963718977	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	906	0.12451930873916642	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	893	0.12273260784114305	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06954389649229382	0.0
2	0.0	0.0	0.0	0.10637742269769845	0.0
3	0.0	0.0	0.0	0.14898336718902472	0.0
4	0.0	0.0	0.0	0.352942146624922	0.0
5	0.0	0.0	0.0	0.3970599149530373	0.0
6	0.0	0.0	0.0	0.6759226935752984	0.0
7	0.0	0.0	0.0	0.8720474767660163	0.0
8	0.0	0.0	0.0	1.069271768201672	0.0
9	0.0	0.0	0.0	1.3082773729449504	0.0
10	0.0	0.0	0.0	1.6998397466733004	0.0
11	0.0	0.0	0.0	2.0203464000725675	0.0
12	0.0	0.0	0.0	2.1658938039961626	0.0
13	0.0	0.0	0.0	2.2377741555089488	0.0
14	0.0	0.0	0.0	2.320374712409875	0.0
15	0.0	0.0	0.0	2.3929422565757466	0.0
16	0.0	0.0	0.0	2.5199354588660223	0.0
17	0.0	0.0	0.0	2.661634583932336	0.0
18	0.0	0.0	0.0	2.826560820672954	0.0
19	0.0	0.0	0.0	2.9160333041047393	0.0
20	0.0	0.0	0.0	3.0237851121086092	0.0
21	0.0	0.0	0.0	3.1396457934188935	0.0
22	0.0	0.0	0.0	3.277221762566692	0.0
23	0.0	0.0	0.0	3.4076509281223974	0.0
24	0.0	0.0	0.0	3.521037715881572	0.0
25	0.0	0.0	0.0	3.6287895238854424	0.0
26	0.0	0.0	0.0	3.7258211265011725	0.0
27	0.0	0.0	0.0	3.8335729345050424	0.0
28	0.0	0.0	0.0	3.9359646398148427	0.0
29	0.0	0.0	0.0	4.051000689941423	0.0
30	0.0	0.0	0.0	4.185690449946262	0.0
31	0.0	0.0	0.0	4.301688569787163	0.0
32	0.0	0.0	0.0	4.423596546444602	0.0
33	0.0	0.0	0.0	4.540006981877355	0.0
34	0.0	0.0	0.0	4.665488360330842	0.0
35	0.0	0.0	0.0	4.795092894702844	0.0
36	0.0	0.0	0.0	4.938991036259033	0.0
37	0.0	0.0	0.0	5.0698325174065895	0.0
38	0.0	0.0	0.0	5.213593220432162	0.0
39	0.0	0.0	0.0	5.3552923454984755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	75	0.0	45.000004	6
TCGGATA	25	3.8893886E-5	45.000004	13
ACTAGCG	25	3.8893886E-5	45.000004	1
ATTGCGT	25	3.8893886E-5	45.000004	33
GATTCGT	25	3.8893886E-5	45.000004	25
TATCGCG	30	2.1643991E-6	45.000004	1
CGATACG	25	3.8893886E-5	45.000004	1
TGCGTCG	25	3.8893886E-5	45.000004	37
TTTAGCG	20	7.031578E-4	45.0	1
CCTACGG	45	3.8380676E-10	45.0	2
ACGATGA	35	1.2111013E-7	45.0	39
CGCTATG	40	6.8084773E-9	45.0	1
CGGTGTA	20	7.031578E-4	45.0	14
ACGTATA	20	7.031578E-4	45.0	42
CGCAATC	20	7.031578E-4	45.0	11
ATACGGA	20	7.031578E-4	45.0	18
CGATATG	20	7.031578E-4	45.0	1
TAATCCG	20	7.031578E-4	45.0	1
ACGCGCT	20	7.031578E-4	45.0	16
ACTACGG	80	0.0	42.1875	2
>>END_MODULE