FastQCFastQC Report
Sat 18 Jun 2016
SRR3554283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712717
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27630.38767140393732713No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA25320.355260222500656No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14560.20428865875235191No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11140.15630327324870882No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9310.13062688275991735No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA9150.12838195244395742No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8780.12319055108830013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG302.1643373E-645.0000041
GGATCGA253.8893082E-545.0000049
CGATTCC253.8893082E-545.00000434
ACTAGCG253.8893082E-545.0000041
CGATATT253.8893082E-545.00000442
TATCACG453.8380676E-1045.01
ACACGAT207.0314814E-445.018
GTCGATG207.0314814E-445.01
CGTTATG207.0314814E-445.01
ACGATAG207.0314814E-445.01
TCGTAAG207.0314814E-445.01
GTATACT207.0314814E-445.037
CGTCCCG207.0314814E-445.01
TATCGCG207.0314814E-445.01
CGATAGG900.042.52
CGAAAGG850.042.352942
ACGGGCT2400.041.2500045
TCATACG603.6379788E-1241.2500041
TACTCGC1150.041.08695645
AATTGCG501.0804797E-940.5000041