##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963073
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31198569578838	34.0	31.0	34.0	30.0	34.0
2	32.497881261337405	34.0	31.0	34.0	31.0	34.0
3	32.59128851083978	34.0	31.0	34.0	31.0	34.0
4	36.10017828347384	37.0	35.0	37.0	35.0	37.0
5	35.92842910142845	37.0	35.0	37.0	35.0	37.0
6	35.90864243935818	37.0	35.0	37.0	35.0	37.0
7	35.96978006859293	37.0	35.0	37.0	35.0	37.0
8	36.08166047641248	37.0	35.0	37.0	35.0	37.0
9	37.68479336457361	39.0	38.0	39.0	35.0	39.0
10	37.3064772867685	39.0	37.0	39.0	34.0	39.0
11	37.285755077756306	39.0	37.0	39.0	34.0	39.0
12	37.22590810873111	39.0	37.0	39.0	34.0	39.0
13	37.204691648504316	39.0	37.0	39.0	34.0	39.0
14	38.4745175080186	40.0	38.0	41.0	34.0	41.0
15	38.49779300219194	40.0	38.0	41.0	34.0	41.0
16	38.48638369054059	40.0	38.0	41.0	34.0	41.0
17	38.449947200264155	40.0	38.0	41.0	34.0	41.0
18	38.38160347138794	40.0	38.0	41.0	34.0	41.0
19	38.392276597931826	40.0	38.0	41.0	34.0	41.0
20	38.301701947827425	40.0	38.0	41.0	34.0	41.0
21	38.26403398288603	40.0	38.0	41.0	34.0	41.0
22	38.18720595427346	40.0	37.0	41.0	34.0	41.0
23	38.147461303556426	40.0	37.0	41.0	34.0	41.0
24	38.07594439881505	40.0	37.0	41.0	33.0	41.0
25	38.02381231744634	40.0	37.0	41.0	33.0	41.0
26	37.923806398891884	40.0	37.0	41.0	33.0	41.0
27	37.833607628912866	40.0	37.0	41.0	33.0	41.0
28	37.760089837426655	40.0	37.0	41.0	33.0	41.0
29	37.66074534329174	40.0	37.0	41.0	33.0	41.0
30	37.644548232584654	40.0	37.0	41.0	33.0	41.0
31	37.59806577486857	40.0	36.0	41.0	33.0	41.0
32	37.53409658457874	40.0	36.0	41.0	33.0	41.0
33	37.431295446970275	40.0	36.0	41.0	32.0	41.0
34	37.33223338210084	40.0	36.0	41.0	32.0	41.0
35	37.296533076931865	40.0	36.0	41.0	32.0	41.0
36	37.20897481291657	39.0	36.0	41.0	31.0	41.0
37	37.15092521543019	39.0	36.0	41.0	31.0	41.0
38	37.12835683276346	39.0	35.0	41.0	31.0	41.0
39	37.0594461686705	39.0	35.0	41.0	31.0	41.0
40	36.954025291956064	39.0	35.0	41.0	31.0	41.0
41	36.880703747275646	39.0	35.0	41.0	31.0	41.0
42	36.80646742251107	39.0	35.0	41.0	31.0	41.0
43	36.73057909421197	39.0	35.0	41.0	31.0	41.0
44	36.642659486871715	39.0	35.0	41.0	30.0	41.0
45	36.535094432093935	39.0	35.0	41.0	30.0	41.0
46	36.43966656733186	39.0	35.0	40.0	30.0	41.0
47	36.31286413387147	39.0	35.0	40.0	30.0	41.0
48	36.22577727752725	39.0	35.0	40.0	30.0	41.0
49	36.079164300110165	38.0	35.0	40.0	29.0	41.0
50	35.94923437787167	38.0	35.0	40.0	29.0	41.0
51	34.93946253295441	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	6.0
12	12.0
13	17.0
14	33.0
15	52.0
16	84.0
17	141.0
18	242.0
19	472.0
20	739.0
21	1209.0
22	1788.0
23	2634.0
24	3303.0
25	4435.0
26	5558.0
27	6913.0
28	8686.0
29	11515.0
30	14710.0
31	19029.0
32	24612.0
33	33342.0
34	51408.0
35	64310.0
36	79571.0
37	115495.0
38	199678.0
39	312906.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.263780627221408	33.772621597739736	20.267622495906334	25.695975279132526
2	22.192917878499347	31.417659928167442	20.694381422799726	25.69504077053349
3	21.8654245316814	30.813240533168308	21.252594559290934	26.068740375859356
4	22.425195182504336	31.238130442863625	19.527491685469325	26.809182689162714
5	19.322211296547614	34.93265827201053	17.14106822639613	28.60406220504572
6	20.945556567363013	37.83150394622214	19.239040031233355	21.983899455181486
7	85.76784937382732	6.2760559168411945	4.19833179831643	3.7577629110150528
8	87.10284682469553	5.357641632565755	4.094809012400929	3.4447025303377834
9	76.7903367657488	11.455102572702174	6.912767775651482	4.841792885897538
10	32.1643322987977	40.55206614659533	14.442830398111047	12.840771156495926
11	25.51623812525115	29.003512713989487	28.10991482473291	17.37033433602645
12	28.391824918775626	24.702488804067812	28.875069698766342	18.030616578390216
13	24.298573420706425	26.91052495501379	27.563019625718923	21.227881998560857
14	19.79195761899669	28.96758605007097	28.201912004593627	23.03854432633871
15	20.327846383399805	30.785724446641115	29.21658067456984	19.66984849538924
16	22.16176759186479	29.425079926443793	28.571250569790656	19.84190191190076
17	21.23027018720284	30.18961179474453	28.123101779408206	20.457016238644425
18	21.15332897921549	30.5970575439245	27.528961978998478	20.720651497861535
19	22.551561511951846	30.051200687798328	27.015501420972242	20.381736379277584
20	24.150609559192294	29.05761037844483	28.0316237709914	18.760156291371473
21	23.593434765588903	29.642508927152978	27.475694988853387	19.288361318404732
22	22.425714353948248	29.77707816541425	27.263042365428163	20.534165115209333
23	22.30879694477989	29.668052162193316	27.734657705075318	20.288493187951484
24	21.03132368989682	28.513103368072823	30.183900908861528	20.271672033168826
25	20.302614651225813	32.150418504100934	26.373182510567734	21.173784334105513
26	22.027198353603517	31.18704397278296	26.41803892332149	20.36771875029203
27	21.548833785185547	29.869698351007663	27.010102037955587	21.571365825851206
28	20.930812098355993	30.30943656399878	28.008572558881827	20.751178778763396
29	20.83788040989624	29.48769200257924	27.466972908595714	22.207454678928805
30	20.913056434974294	30.65499707706477	26.987985334445053	21.443961153515883
31	21.59296335791783	31.01229086476311	26.222518957545276	21.172226819773787
32	22.685300075902866	29.039231709330444	27.353066693802024	20.922401520964662
33	21.203169437830777	31.674130621458602	26.31223178305279	20.81046815765783
34	20.356919984258724	30.26935652852899	28.665532104004576	20.708191383207712
35	22.72776830001464	28.657536863768375	28.53646608304874	20.078228753168244
36	21.70250853258268	32.04918007253863	26.603798465952217	19.644512928926467
37	20.81586754067449	32.45942934751571	27.531038664774115	19.193664447035687
38	20.359723510055833	30.033029687261504	29.585815405478087	20.021431397204573
39	21.4392886105207	29.448027304264578	28.345930163134053	20.76675392208067
40	21.808523341428945	30.453973893983115	28.812976794074803	18.92452597051314
41	19.181515835248213	29.344504518349073	30.491665740810923	20.982313905591788
42	19.37755497246834	30.084531494497302	28.618806674052742	21.919106858981614
43	20.993424174491444	29.528706546648074	28.142207288544068	21.335661990316414
44	21.370134974191988	30.158876845265105	27.136156864536748	21.33483131600616
45	19.9881005905056	30.05244669926371	28.010130073213556	21.949322637017133
46	18.792967926626538	31.36885781243997	28.305746293375478	21.532427967558014
47	20.181232367639836	29.380535016556376	29.515831094839122	20.922401520964662
48	20.204802751193316	28.958967804102077	29.847270144630784	20.988959300073827
49	19.32376881087934	31.403953802048235	28.22485938241442	21.047418004658006
50	19.346404685833786	29.420303549159826	29.559026158972372	21.67426560603402
51	20.238756563624978	28.35184871759462	28.697824567815733	22.711570150964672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4249.0
1	3398.0
2	2547.0
3	1879.5
4	1212.0
5	1220.0
6	1228.0
7	1433.5
8	1639.0
9	1716.5
10	1794.0
11	2042.5
12	2291.0
13	2529.0
14	2767.0
15	2849.0
16	2931.0
17	3371.0
18	3811.0
19	4422.0
20	5033.0
21	5116.5
22	5200.0
23	6927.5
24	8655.0
25	9768.5
26	14395.5
27	17909.0
28	20126.5
29	22344.0
30	25609.0
31	28874.0
32	33741.0
33	38608.0
34	40703.5
35	42799.0
36	46910.5
37	51022.0
38	54139.0
39	57256.0
40	57873.0
41	58490.0
42	59383.5
43	60277.0
44	61804.0
45	63331.0
46	63991.5
47	64652.0
48	63705.0
49	62758.0
50	59504.0
51	56250.0
52	52121.5
53	47993.0
54	45784.0
55	43575.0
56	40036.5
57	36498.0
58	35564.5
59	34631.0
60	34506.0
61	34381.0
62	31426.5
63	28472.0
64	24149.0
65	19826.0
66	16548.5
67	13271.0
68	11254.5
69	9238.0
70	7879.5
71	6521.0
72	5107.0
73	3693.0
74	3108.5
75	2093.5
76	1663.0
77	1298.5
78	934.0
79	726.5
80	519.0
81	379.0
82	239.0
83	179.5
84	120.0
85	97.5
86	75.0
87	61.0
88	47.0
89	30.0
90	13.0
91	13.5
92	14.0
93	11.5
94	9.0
95	6.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	963073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.95151641873251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.83678056282088	16.717145649752002
2	8.155898468517506	3.7438047521938973
3	3.3043263727101286	2.275179029883221
4	1.9666435717783557	1.8054980890986272
5	1.3828103540624832	1.58687972726292
6	1.072471799653518	1.4768912471025193
7	0.8185063067036411	1.3150172656001309
8	0.7407851103913337	1.3601713299119416
9	0.6287433427820069	1.2987551839526994
>10	7.6193564574333505	38.4634250101117
>50	1.1733913762294985	18.552079324227392
>100	0.2925516303906714	9.37879349426888
>500	0.004549792074504999	0.6358415568464072
>1k	0.003184854452153499	1.39051833978761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3161	0.3282201868394192	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3093	0.3211594552022536	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2460	0.2554323504033443	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1296	0.13456923826127407	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1209	0.12553565513725334	No Hit
AGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAGGTGAA	1091	0.11328320906099539	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1006	0.10445729451453835	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07590286509953036	0.0
2	0.0	0.0	0.0	0.11515222625906862	0.0
3	0.0	0.0	0.0	0.16675786778364673	0.0
4	0.0	0.0	0.0	0.3659120336672298	0.0
5	0.0	0.0	0.0	0.41346813792931586	0.0
6	0.0	0.0	0.0	0.7438688448331539	0.0
7	0.0	0.0	0.0	1.0030392296326447	0.0
8	0.0	0.0	0.0	1.2940867410881627	0.0
9	0.0	0.0	0.0	1.617426716354835	0.0
10	0.0	0.0	0.0	2.1290182571829965	0.0
11	0.0	0.0	0.0	2.5042753768405928	0.0
12	1.0383428878184728E-4	0.0	0.0	2.6852585421873525	0.0
13	1.0383428878184728E-4	0.0	0.0	2.774036859095832	0.0
14	1.0383428878184728E-4	0.0	0.0	2.8652033646462938	0.0
15	1.0383428878184728E-4	0.0	0.0	2.945155767008316	0.0
16	1.0383428878184728E-4	0.0	0.0	3.084605216842337	0.0
17	1.0383428878184728E-4	0.0	0.0	3.2455483644542005	0.0
18	1.0383428878184728E-4	0.0	0.0	3.4385763072996545	0.0
19	1.0383428878184728E-4	0.0	0.0	3.550094333451358	0.0
20	1.0383428878184728E-4	0.0	0.0	3.6736571371017566	0.0
21	1.0383428878184728E-4	0.0	0.0	3.7999196322604827	0.0
22	1.0383428878184728E-4	0.0	0.0	3.956709408321072	0.0
23	1.0383428878184728E-4	0.0	0.0	4.098650881085858	0.0
24	1.0383428878184728E-4	0.0	0.0	4.22470570766702	0.0
25	1.0383428878184728E-4	0.0	0.0	4.3369505738401966	0.0
26	1.0383428878184728E-4	0.0	0.0	4.440369525466917	0.0
27	1.0383428878184728E-4	0.0	0.0	4.554898745993294	0.0
28	1.0383428878184728E-4	0.0	0.0	4.674827349536328	0.0
29	1.0383428878184728E-4	0.0	0.0	4.802543524738	0.0
30	1.0383428878184728E-4	0.0	0.0	4.9614099865742265	0.0
31	1.0383428878184728E-4	0.0	0.0	5.087672481732953	0.0
32	1.0383428878184728E-4	0.0	0.0	5.23200214313972	0.0
33	2.0766857756369456E-4	0.0	0.0	5.372386101572778	0.0
34	2.0766857756369456E-4	0.0	0.0	5.513704568604872	0.0
35	2.0766857756369456E-4	0.0	0.0	5.660941590097532	0.0
36	2.0766857756369456E-4	0.0	0.0	5.810047628788264	0.0
37	2.0766857756369456E-4	0.0	0.0	5.957803821724833	0.0
38	2.0766857756369456E-4	0.0	0.0	6.115528106384459	0.0
39	2.0766857756369456E-4	0.0	0.0	6.27408306535434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.165134E-6	45.000004	1
GTATACG	35	1.211647E-7	45.000004	1
ATACCCG	30	2.165134E-6	45.000004	1
TAATACG	20	7.032717E-4	45.0	1
CACGCTA	20	7.032717E-4	45.0	24
TTATCCG	20	7.032717E-4	45.0	1
TCTAGCG	75	0.0	45.0	1
CGTAGCG	40	6.8139343E-9	45.0	1
TCTCGAC	20	7.032717E-4	45.0	23
CACGTTG	20	7.032717E-4	45.0	1
TCGACGT	20	7.032717E-4	45.0	25
CGTTTAC	25	3.890333E-5	45.0	32
TATACGG	250	0.0	43.199997	2
ATATACG	130	0.0	41.53846	1
AACCGCG	60	3.6379788E-12	41.250004	1
CTTAACG	115	0.0	41.086956	1
CTACGAA	115	0.0	41.086956	10
GTTAGCG	50	1.0804797E-9	40.5	1
TTATACG	50	1.0804797E-9	40.5	1
TCTAACG	100	0.0	40.5	1
>>END_MODULE