FastQCFastQC Report
Sat 18 Jun 2016
SRR3554279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486171
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48951.0068473849735997No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT13950.28693607804661325TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9440.1941703639254501No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.19108502975290587No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.10531273975617633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA302.1629075E-645.00000419
TGACGTC302.1629075E-645.00000431
ACTACCG302.1629075E-645.0000041
GGACGAC302.1629075E-645.0000041
CGATCTG302.1629075E-645.0000041
TGAGACG351.2099918E-745.0000041
TGATCGG207.029267E-445.02
AACCGGT253.8874736E-545.03
TCGCAAG502.1827873E-1145.01
ACCGGAA207.029267E-445.011
TCACCGT207.029267E-445.014
TCCGACG406.8012014E-945.01
CCGGCTA253.8874736E-545.015
CGGTTCG207.029267E-445.030
GTCTCGC253.8874736E-545.027
GTCTATC207.029267E-445.024
TGTATCG207.029267E-445.018
CGATTCG253.8874736E-545.017
TCGATAA253.8874736E-545.021
CCTCGCT207.029267E-445.028