Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554279_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 486171 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4895 | 1.0068473849735997 | No Hit |
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT | 1395 | 0.28693607804661325 | TruSeq Adapter, Index 20 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 944 | 0.1941703639254501 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 929 | 0.19108502975290587 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 512 | 0.10531273975617633 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGATA | 30 | 2.1629075E-6 | 45.000004 | 19 |
TGACGTC | 30 | 2.1629075E-6 | 45.000004 | 31 |
ACTACCG | 30 | 2.1629075E-6 | 45.000004 | 1 |
GGACGAC | 30 | 2.1629075E-6 | 45.000004 | 1 |
CGATCTG | 30 | 2.1629075E-6 | 45.000004 | 1 |
TGAGACG | 35 | 1.2099918E-7 | 45.000004 | 1 |
TGATCGG | 20 | 7.029267E-4 | 45.0 | 2 |
AACCGGT | 25 | 3.8874736E-5 | 45.0 | 3 |
TCGCAAG | 50 | 2.1827873E-11 | 45.0 | 1 |
ACCGGAA | 20 | 7.029267E-4 | 45.0 | 11 |
TCACCGT | 20 | 7.029267E-4 | 45.0 | 14 |
TCCGACG | 40 | 6.8012014E-9 | 45.0 | 1 |
CCGGCTA | 25 | 3.8874736E-5 | 45.0 | 15 |
CGGTTCG | 20 | 7.029267E-4 | 45.0 | 30 |
GTCTCGC | 25 | 3.8874736E-5 | 45.0 | 27 |
GTCTATC | 20 | 7.029267E-4 | 45.0 | 24 |
TGTATCG | 20 | 7.029267E-4 | 45.0 | 18 |
CGATTCG | 25 | 3.8874736E-5 | 45.0 | 17 |
TCGATAA | 25 | 3.8874736E-5 | 45.0 | 21 |
CCTCGCT | 20 | 7.029267E-4 | 45.0 | 28 |