##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486171
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.281740375300046	34.0	31.0	34.0	30.0	34.0
2	32.468954339111136	34.0	31.0	34.0	30.0	34.0
3	32.54856624521002	34.0	31.0	34.0	31.0	34.0
4	36.07150364789344	37.0	35.0	37.0	35.0	37.0
5	35.90311433631376	37.0	35.0	37.0	35.0	37.0
6	35.89091081121663	37.0	35.0	37.0	35.0	37.0
7	35.979540120657134	37.0	35.0	37.0	35.0	37.0
8	36.10877859847667	37.0	35.0	37.0	35.0	37.0
9	37.750384124104485	39.0	38.0	39.0	35.0	39.0
10	37.26956564665519	39.0	37.0	39.0	34.0	39.0
11	37.27840615750425	39.0	37.0	39.0	34.0	39.0
12	37.30426125787017	39.0	37.0	39.0	34.0	39.0
13	37.292837293873966	39.0	37.0	39.0	34.0	39.0
14	38.547587165832596	40.0	38.0	41.0	34.0	41.0
15	38.530504698963945	40.0	38.0	41.0	34.0	41.0
16	38.527826628902176	40.0	38.0	41.0	34.0	41.0
17	38.48022403639872	40.0	38.0	41.0	34.0	41.0
18	38.389766152238614	40.0	38.0	41.0	34.0	41.0
19	38.376704904241514	40.0	38.0	41.0	34.0	41.0
20	38.27242677987786	40.0	38.0	41.0	34.0	41.0
21	38.21892297154705	40.0	37.0	41.0	34.0	41.0
22	38.18192981481824	40.0	37.0	41.0	34.0	41.0
23	38.10332578454906	40.0	37.0	41.0	33.0	41.0
24	38.08394165838769	40.0	37.0	41.0	33.0	41.0
25	38.01055801353844	40.0	37.0	41.0	33.0	41.0
26	37.955719283955645	40.0	37.0	41.0	33.0	41.0
27	37.905928161079125	40.0	37.0	41.0	33.0	41.0
28	37.797157790160256	40.0	37.0	41.0	33.0	41.0
29	37.760302445024486	40.0	37.0	41.0	33.0	41.0
30	37.71243040000329	40.0	37.0	41.0	33.0	41.0
31	37.62088236443556	40.0	37.0	41.0	33.0	41.0
32	37.59154083645466	40.0	36.0	41.0	33.0	41.0
33	37.50868727258516	40.0	36.0	41.0	33.0	41.0
34	37.39740338276039	40.0	36.0	41.0	32.0	41.0
35	37.3428629021476	40.0	36.0	41.0	32.0	41.0
36	37.275668437648484	39.0	36.0	41.0	32.0	41.0
37	37.21782047880273	39.0	36.0	41.0	32.0	41.0
38	37.19026844464191	39.0	36.0	41.0	32.0	41.0
39	37.103031649357945	39.0	35.0	41.0	31.0	41.0
40	37.05456927706506	39.0	35.0	41.0	31.0	41.0
41	36.963194020211	39.0	35.0	41.0	31.0	41.0
42	36.87250576443268	39.0	35.0	41.0	31.0	41.0
43	36.80376863284729	39.0	35.0	41.0	31.0	41.0
44	36.72848853592666	39.0	35.0	41.0	31.0	41.0
45	36.589952917800524	39.0	35.0	41.0	30.0	41.0
46	36.447599712858235	39.0	35.0	40.0	30.0	41.0
47	36.38713539063416	39.0	35.0	40.0	30.0	41.0
48	36.294978515789715	39.0	35.0	40.0	30.0	41.0
49	36.21190075097034	39.0	35.0	40.0	30.0	41.0
50	36.08716891793217	39.0	35.0	40.0	29.0	41.0
51	35.113908480760884	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	3.0
13	3.0
14	5.0
15	16.0
16	27.0
17	47.0
18	100.0
19	200.0
20	368.0
21	680.0
22	934.0
23	1312.0
24	1665.0
25	2098.0
26	2551.0
27	3119.0
28	4201.0
29	5421.0
30	7208.0
31	9332.0
32	12312.0
33	16544.0
34	24679.0
35	34619.0
36	40537.0
37	59983.0
38	100177.0
39	157937.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.380835138253826	32.740743483260005	22.21625724282197	25.6621641356642
2	21.7509888496023	33.04434036583836	21.26576862873351	23.93890215582583
3	20.051175409475267	33.1823576478235	21.714787595311115	25.051679347390117
4	20.369581896081833	31.949046734585156	20.140033033644542	27.54133833568847
5	18.22054380043236	34.10857496642128	18.500280765409702	29.170600467736662
6	20.366085184019614	37.431479870251415	20.17109206431482	22.031342881414155
7	85.70194437759554	5.906563739918671	4.5736993773795644	3.8177925051062283
8	87.20676469801778	5.49498016130127	3.677101266838211	3.6211538738427427
9	78.39484461228662	10.802166315967016	6.894899119857005	3.9080899518893557
10	28.79583521024496	43.77533830689202	14.364904529476256	13.063921953386771
11	20.530224961998968	29.249173644664122	32.318464079511116	17.902137313825794
12	22.97751202766105	25.470256350131947	33.13854590257338	18.413685719633627
13	22.075154626664283	26.916661010220682	30.53987177351179	20.468312589603247
14	18.307550224098105	29.62990388155608	30.9010615606443	21.161484333701516
15	17.802583864525033	30.58039249564454	32.424393886101804	19.192629753728628
16	20.501634198666725	30.892422624961174	28.982395083211465	19.623548093160636
17	19.735648568096412	29.640599706687563	29.964354105859876	20.659397619356152
18	20.063928124055117	29.113418941072172	30.921424766183094	19.901228168689617
19	20.293888364382077	31.05861929238889	28.880373366572666	19.767118976656363
20	20.353949536274275	29.69757554440721	31.14007211454406	18.808402804774452
21	21.877898928566285	29.945842100824606	29.55955826242207	18.616700708187036
22	20.767178626450363	28.382811809013702	30.84634830131785	20.003661263218085
23	20.04932420897174	28.73618541624243	29.83209611433014	21.382394260455683
24	17.593809585516208	29.095729691816253	31.74356347869371	21.56689724397383
25	18.26312141201347	32.99538639696733	28.96306032239685	19.778431868622356
26	20.015385533073754	30.323281314599182	30.234629379374745	19.426703772952315
27	17.73635202428775	30.833595586738	30.039841948614786	21.39021044035946
28	17.698505258437873	30.741035561561674	30.834212653572507	20.726246526427943
29	18.93695839529713	29.025795450571916	30.329863360833947	21.707382793297008
30	18.40792642917821	30.269596499996915	30.539254706677283	20.783222364147594
31	19.258655905021072	31.18038714773197	28.542837808096326	21.018119139150627
32	20.31260605836218	29.77758854394853	29.202276565241448	20.707528832447842
33	18.36246917236939	30.096406408444764	30.77271165906646	20.768412760119382
34	18.561987448860588	29.834358692723338	30.390747288505487	21.212906569910587
35	18.489996318167886	29.99890984859237	31.76248686161865	19.748606971621097
36	18.60147972626915	32.21027169452723	29.74591244644374	19.44233613275987
37	18.018351567658293	31.245179165355403	30.307854643736466	20.428614623249842
38	19.524611710694384	29.072898218939425	31.31984425233097	20.082645818035218
39	18.55026317900492	29.382254391973195	30.633665932357136	21.433816496664754
40	20.241643372393664	28.99658762040517	30.413990139271984	20.347778867929186
41	17.991612004829577	28.841498155998607	31.644421407282625	21.52246843188919
42	18.500486454354537	29.5036108694266	30.75399396508636	21.241908711132503
43	18.278753771821027	29.86315514500042	30.74885174146545	21.109239341713103
44	19.541272515226122	28.63724903377618	30.74185831734102	21.079620133656675
45	19.134214093395123	29.642039529301417	30.008577228999673	21.215169148303787
46	18.066482780749983	30.84634830131785	30.851696213883596	20.235472704048576
47	18.24975163059911	29.67803509464777	30.18896643362109	21.883246841132028
48	17.61972639256558	28.79233849818274	32.52559284696125	21.06234226229043
49	19.173089303969178	28.980955260597607	29.762573250975482	22.08338218445773
50	18.336140987430348	29.03607989781373	31.10757326125993	21.52020585349599
51	19.042888201887813	27.08388612237258	31.561940140403273	22.31128553533633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5692.0
1	4313.5
2	2935.0
3	1890.0
4	845.0
5	688.5
6	532.0
7	721.0
8	910.0
9	1106.5
10	1303.0
11	1451.5
12	1600.0
13	1803.0
14	2006.0
15	1903.0
16	1800.0
17	1847.0
18	1894.0
19	2100.0
20	2306.0
21	2798.0
22	3290.0
23	3391.5
24	3493.0
25	4392.5
26	7141.0
27	8990.0
28	10807.5
29	12625.0
30	13498.0
31	14371.0
32	17391.0
33	20411.0
34	23434.0
35	26457.0
36	27702.0
37	28947.0
38	29665.0
39	30383.0
40	30314.5
41	30246.0
42	30858.0
43	31470.0
44	32773.0
45	34076.0
46	34651.5
47	35227.0
48	37550.0
49	39873.0
50	36932.0
51	33991.0
52	29481.0
53	24971.0
54	21859.0
55	18747.0
56	17734.5
57	16722.0
58	14425.0
59	12128.0
60	10427.5
61	8727.0
62	8273.5
63	7820.0
64	6459.0
65	5098.0
66	4452.0
67	3806.0
68	3254.0
69	2702.0
70	2286.5
71	1871.0
72	1536.0
73	1201.0
74	898.5
75	468.5
76	341.0
77	306.0
78	271.0
79	183.0
80	95.0
81	83.0
82	71.0
83	45.5
84	20.0
85	13.5
86	7.0
87	5.0
88	3.0
89	4.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.96142762510582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90843218035607	19.44729840560462
2	9.29875291818982	4.82817801778653
3	3.5109788635280115	2.734500709762762
4	1.8515205381616269	1.9227246579152024
5	1.2194773451471592	1.5829686418247082
6	0.8373228819613612	1.304285843933094
7	0.6490540499231731	1.1795258819303596
8	0.5442790775514074	1.1304209503768177
9	0.4490616487578073	1.0492453344092854
>10	5.349035376988153	30.693622731463186
>50	0.9790430754209496	17.87886477577016
>100	0.3990750947623482	14.461528045838449
>500	0.00238016955126649	0.4912511663484503
>1k	0.0015867797008443268	1.2955848370363743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4895	1.0068473849735997	No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	1395	0.28693607804661325	TruSeq Adapter, Index 20 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	944	0.1941703639254501	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	929	0.19108502975290587	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	512	0.10531273975617633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33445022430379434	0.0
2	0.0	0.0	0.0	0.47411301784762977	0.0
3	0.0	0.0	0.0	0.6205635465710625	0.0
4	0.0	0.0	0.0	1.3118840901658058	0.0
5	0.0	0.0	0.0	1.4077351384595131	0.0
6	0.0	0.0	0.0	2.063677183542416	0.0
7	0.0	0.0	0.0	2.525448864699869	0.0
8	0.0	0.0	0.0	2.9613037388079504	0.0
9	0.0	0.0	0.0	3.582690041158358	0.0
10	0.0	0.0	0.0	4.593445516083847	0.0
11	0.0	0.0	0.0	5.416201295428975	0.0
12	0.0	0.0	0.0	5.913145786153431	0.0
13	0.0	0.0	0.0	6.142077581756213	0.0
14	0.0	0.0	0.0	6.3442698145302785	0.0
15	0.0	0.0	0.0	6.519516795530791	0.0
16	0.0	0.0	0.0	6.900452721367585	0.0
17	0.0	0.0	0.0	7.373948672380705	0.0
18	0.0	0.0	0.0	8.03091916218779	0.0
19	0.0	0.0	0.0	8.29646358997143	0.0
20	0.0	0.0	0.0	8.621657811757592	0.0
21	0.0	0.0	0.0	8.970094884310253	0.0
22	0.0	0.0	0.0	9.35144218803672	0.0
23	0.0	0.0	0.0	9.72538468974908	0.0
24	0.0	0.0	0.0	10.016228857747583	0.0
25	0.0	0.0	0.0	10.270460393565227	0.0
26	0.0	0.0	0.0	10.497540988664483	0.0
27	0.0	0.0	0.0	10.782831555152406	0.0
28	0.0	0.0	0.0	11.067916432695492	0.0
29	0.0	0.0	0.0	11.324821924796009	0.0
30	0.0	0.0	0.0	11.679429665693759	0.0
31	0.0	0.0	0.0	11.954435784939866	0.0
32	0.0	0.0	0.0	12.238697906703608	0.0
33	0.0	0.0	0.0	12.4914896199074	0.0
34	0.0	0.0	0.0	12.747160978338897	0.0
35	0.0	0.0	0.0	13.049112349358559	0.0
36	0.0	0.0	0.0	13.337693938963863	0.0
37	0.0	0.0	0.0	13.618665037610224	0.0
38	2.0568894483628187E-4	0.0	0.0	13.905395426712001	0.0
39	2.0568894483628187E-4	0.0	0.0	14.196239594710503	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGATA	30	2.1629075E-6	45.000004	19
TGACGTC	30	2.1629075E-6	45.000004	31
ACTACCG	30	2.1629075E-6	45.000004	1
GGACGAC	30	2.1629075E-6	45.000004	1
CGATCTG	30	2.1629075E-6	45.000004	1
TGAGACG	35	1.2099918E-7	45.000004	1
TGATCGG	20	7.029267E-4	45.0	2
AACCGGT	25	3.8874736E-5	45.0	3
TCGCAAG	50	2.1827873E-11	45.0	1
ACCGGAA	20	7.029267E-4	45.0	11
TCACCGT	20	7.029267E-4	45.0	14
TCCGACG	40	6.8012014E-9	45.0	1
CCGGCTA	25	3.8874736E-5	45.0	15
CGGTTCG	20	7.029267E-4	45.0	30
GTCTCGC	25	3.8874736E-5	45.0	27
GTCTATC	20	7.029267E-4	45.0	24
TGTATCG	20	7.029267E-4	45.0	18
CGATTCG	25	3.8874736E-5	45.0	17
TCGATAA	25	3.8874736E-5	45.0	21
CCTCGCT	20	7.029267E-4	45.0	28
>>END_MODULE