FastQCFastQC Report
Sat 18 Jun 2016
SRR3554278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367469
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83402.269579202599403No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15460.4207157610573953No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.3638402150929738No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT10100.2748531168615584No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8030.21852183449488255No Hit
GAACTTGGGATGAAACTCAGGAGTTACCTCCCATGCGAGAAATCTTAGATT7080.1926693136019637No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGT4440.12082651869953656No Hit
AATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT4370.11892159610742675No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCC3730.1015051609795656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG453.8380676E-1045.0000041
TCCGGAT253.885609E-545.043
CTTCGAA207.027015E-445.012
CTAGACG406.7939254E-945.014
CGAACCG351.2089185E-745.01
CACGGGT1100.045.04
CAACGAA253.885609E-545.038
ACGTTAC253.885609E-545.022
TTAGCGA207.027015E-445.044
CTATCAT207.027015E-445.015
AACCACG253.885609E-545.01
GGTCCGT253.885609E-545.021
ACCGAAA207.027015E-445.044
ACTTACC207.027015E-445.011
TATGCAC207.027015E-445.032
CGTTCAT207.027015E-445.039
TAGCCGT253.885609E-545.044
GCACCGA253.885609E-545.09
CGTTATT207.027015E-445.019
ATGTACG207.027015E-445.01