##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367469
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07154344992367	33.0	31.0	34.0	30.0	34.0
2	32.25488408546027	34.0	31.0	34.0	30.0	34.0
3	32.35719747788249	34.0	31.0	34.0	30.0	34.0
4	35.92152535315904	37.0	35.0	37.0	35.0	37.0
5	35.73313939407134	37.0	35.0	37.0	35.0	37.0
6	35.707790317006335	37.0	35.0	37.0	33.0	37.0
7	35.66239329031837	37.0	35.0	37.0	35.0	37.0
8	35.821631212428805	37.0	35.0	37.0	35.0	37.0
9	37.47565100729585	39.0	37.0	39.0	35.0	39.0
10	37.097597348347755	39.0	37.0	39.0	33.0	39.0
11	37.027041736853995	39.0	37.0	39.0	33.0	39.0
12	36.814931327540556	39.0	35.0	39.0	33.0	39.0
13	36.763438548557836	39.0	35.0	39.0	33.0	39.0
14	37.93253308442345	40.0	37.0	41.0	33.0	41.0
15	37.92736258024487	40.0	37.0	41.0	33.0	41.0
16	37.898026772326375	40.0	37.0	41.0	33.0	41.0
17	37.79078779434456	39.0	36.0	41.0	33.0	41.0
18	37.642010618582795	39.0	36.0	41.0	33.0	41.0
19	37.5519322718379	39.0	35.0	41.0	32.0	41.0
20	37.49108632292792	39.0	35.0	41.0	32.0	41.0
21	37.41608407784058	39.0	35.0	41.0	32.0	41.0
22	37.382984687143676	39.0	35.0	41.0	32.0	41.0
23	37.29229132253333	39.0	35.0	41.0	32.0	41.0
24	37.2237086665814	39.0	35.0	41.0	32.0	41.0
25	37.18469585189499	39.0	35.0	41.0	32.0	41.0
26	37.08334580604078	39.0	35.0	41.0	32.0	41.0
27	36.98059700274037	39.0	35.0	41.0	31.0	41.0
28	36.90769561514033	39.0	35.0	41.0	31.0	41.0
29	36.76063014839347	39.0	35.0	41.0	31.0	41.0
30	36.66924829033197	39.0	35.0	41.0	31.0	41.0
31	36.59917435212222	39.0	35.0	41.0	31.0	41.0
32	36.48621788504609	39.0	35.0	40.0	30.0	41.0
33	36.35595111424365	38.0	35.0	40.0	30.0	41.0
34	36.091425399149315	38.0	35.0	40.0	30.0	41.0
35	36.04976746337786	38.0	35.0	40.0	29.0	41.0
36	36.015802693560545	38.0	35.0	40.0	30.0	41.0
37	35.9385090987267	38.0	35.0	40.0	29.0	41.0
38	35.84697212553984	38.0	35.0	40.0	29.0	41.0
39	35.78746778639831	38.0	35.0	40.0	29.0	41.0
40	35.68652321692442	38.0	35.0	40.0	29.0	41.0
41	35.56323934808106	38.0	34.0	40.0	28.0	41.0
42	35.44770851418759	38.0	34.0	40.0	27.0	41.0
43	35.40117669789833	38.0	34.0	40.0	27.0	41.0
44	35.32028552068338	38.0	34.0	40.0	27.0	41.0
45	35.10782950398537	37.0	34.0	40.0	26.0	41.0
46	34.96075587328455	37.0	34.0	40.0	26.0	41.0
47	34.82596082934887	37.0	34.0	40.0	25.0	41.0
48	34.720615888687206	37.0	33.0	40.0	25.0	41.0
49	34.61671324655958	37.0	33.0	40.0	25.0	41.0
50	34.51050564809549	37.0	33.0	40.0	24.0	41.0
51	33.43185683690325	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	1.0
13	3.0
14	8.0
15	22.0
16	36.0
17	127.0
18	212.0
19	343.0
20	639.0
21	875.0
22	1345.0
23	1777.0
24	2302.0
25	2885.0
26	3463.0
27	4031.0
28	4946.0
29	6086.0
30	7703.0
31	9910.0
32	12736.0
33	17319.0
34	27198.0
35	35411.0
36	32903.0
37	44138.0
38	66854.0
39	84153.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.907154072860568	33.67168386993188	17.919062560379242	26.5020994968283
2	22.143636606081056	34.11444230669798	19.461505596390445	24.28041549083052
3	20.557380350451332	34.122878392463036	21.618150102457623	23.70159115462801
4	19.834870424443967	34.58359752795474	19.01031107385929	26.571220973742005
5	18.716408731076637	36.67656319308568	17.892393644089704	26.714634431747985
6	21.27961814465982	38.52107252584572	19.716493091934286	20.482816237560176
7	78.47791242254448	10.646612367301731	6.213857495462202	4.661617714691579
8	79.76210238142536	9.581760638312348	5.706603822363247	4.949533157899034
9	72.35821252949228	13.387251713750004	9.087297159760416	5.167238596997298
10	35.53034405623332	34.79749312186878	15.80432635133848	13.867836470559421
11	27.37645896660671	29.852314072751717	25.28158837888366	17.489638581757916
12	26.700211446407724	28.21544130253164	27.369655671634884	17.714691579425747
13	24.04093950782243	28.69303260955346	26.564145546971307	20.7018823356528
14	19.582876378687725	30.198193589119082	29.020679295396345	21.198250736796844
15	19.030720958774754	30.661906174398386	29.597326577207873	20.710046289618987
16	22.742054431802412	30.737014550887288	27.36911140803714	19.15181960927316
17	22.462847206158887	30.40365309726807	28.64921938993493	18.484280306638112
18	23.586479403704804	29.085718795326954	27.170183063061103	20.157618737907143
19	22.21983350976545	32.28653301366918	26.287115375718766	19.2065181008466
20	23.50946610462379	30.892401808043672	27.35196710470815	18.246164982624386
21	22.44679143002539	33.067007012836456	25.62882855424539	18.85737300289276
22	22.208676106011662	30.82110327673899	27.933784890698266	19.036435726551083
23	22.487066936258568	30.74436210945685	26.684699933871975	20.083871020412605
24	20.53043930236292	29.827277947255414	29.477588585703828	20.164694164677837
25	20.02372989286171	32.705071720335596	25.7529206545314	21.518277732271294
26	20.94571242744286	30.79252943785734	26.281128476143568	21.980629658556232
27	18.720490708059728	30.991729914632256	27.03847127240665	23.249308104901363
28	19.845755696398882	31.759141587453637	26.964995686710985	21.430107029436495
29	21.51963839126566	32.16135238618768	25.931711246390854	20.38729797615581
30	22.056554430441754	30.6126503188024	26.615850588757144	20.7149446619987
31	22.29956812683519	31.12235317809121	26.024780321605363	20.553298373468238
32	24.824406956777306	29.64903161899371	25.323224544111206	20.203336880117778
33	23.818879959942198	30.332898829561135	25.47997246026195	20.368248750234713
34	20.85155482503286	31.38632102299786	27.67172196838373	20.090402183585553
35	20.879856532115635	30.89294607164142	28.250274172787364	19.976923223455582
36	21.506303933120886	34.53815151754298	25.01734840217814	18.938196147157992
37	21.458953000117017	33.60882142439226	25.454936334765655	19.477289240725067
38	21.727547085604503	33.540244211076306	25.88653736777796	18.84567133554123
39	21.090214412644332	31.553409947505777	27.24311438515902	20.113261254690872
40	22.03151830494545	31.489731106569536	26.143701917712786	20.33504867077223
41	18.907717385684233	31.17079263829058	27.87772574013046	22.043764235894727
42	20.595206670494655	31.364006215490285	25.755097708922385	22.285689405092672
43	19.66968642252816	29.987019313193763	26.83192323706217	23.5113710272159
44	21.345201908188173	28.884069132362182	26.777224745488738	22.993504213960904
45	20.605275547052948	30.63251594012012	27.37210485782474	21.39010365500219
46	19.87378527168278	31.80785317945187	28.085090170871556	20.233271377993788
47	20.145100675158993	31.592596926543465	27.13997643338622	21.122325964911326
48	18.86907467024429	29.94211756637975	29.83925174640582	21.34955601697014
49	20.237625486775755	30.149481997120848	28.816036182643977	20.79685633345942
50	20.190546685570755	29.860478026717903	26.90294963656798	23.046025651143363
51	19.878139380464745	27.557154481058266	29.47595579491059	23.088750343566396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9644.0
1	7218.5
2	4793.0
3	3052.0
4	1311.0
5	1142.0
6	973.0
7	1146.0
8	1319.0
9	1334.5
10	1350.0
11	1437.0
12	1524.0
13	1704.0
14	1884.0
15	2054.5
16	2225.0
17	2017.5
18	1810.0
19	1983.0
20	2156.0
21	2325.5
22	2495.0
23	2540.0
24	2585.0
25	3145.0
26	4838.0
27	5971.0
28	6971.0
29	7971.0
30	8584.0
31	9197.0
32	10425.5
33	11654.0
34	12918.5
35	14183.0
36	13353.0
37	12523.0
38	13069.0
39	13615.0
40	14194.5
41	14774.0
42	17404.0
43	20034.0
44	20560.0
45	21086.0
46	22274.0
47	23462.0
48	24332.5
49	25203.0
50	24851.0
51	24499.0
52	22594.5
53	20690.0
54	19056.0
55	17422.0
56	16617.5
57	15813.0
58	15544.0
59	15275.0
60	14788.5
61	14302.0
62	13553.0
63	12804.0
64	11291.5
65	9779.0
66	8284.5
67	6790.0
68	5683.0
69	4576.0
70	3694.5
71	2813.0
72	2398.5
73	1984.0
74	1623.5
75	1077.5
76	892.0
77	707.5
78	523.0
79	390.0
80	257.0
81	191.0
82	125.0
83	102.0
84	79.0
85	83.5
86	88.0
87	59.5
88	31.0
89	21.0
90	11.0
91	7.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.460158906199467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83206218148251	21.09820636594508
2	8.448659448501951	4.640030620004543
3	3.043784762218744	2.507484397403858
4	1.6117951934393466	1.770406085443719
5	1.1008577072815848	1.5114863787533368
6	0.8008964110608962	1.3195645629082233
7	0.6539154196063559	1.2569634933523246
8	0.5829246018067762	1.2805761756757699
9	0.48293753459909305	1.193538729767337
>10	5.403301111745292	32.13548803098018
>50	0.6919105050569466	13.510298306169721
>100	0.34095589916823527	14.00232071328788
>500	0.0019997413440952213	0.41486933993054603
>1k	0.0029996120161428323	1.0688857315351528
>5k	9.998706720476106E-4	2.2898810688423255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8340	2.269579202599403	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1546	0.4207157610573953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1337	0.3638402150929738	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	1010	0.2748531168615584	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.21852183449488255	No Hit
GAACTTGGGATGAAACTCAGGAGTTACCTCCCATGCGAGAAATCTTAGATT	708	0.1926693136019637	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGT	444	0.12082651869953656	No Hit
AATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	437	0.11892159610742675	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCC	373	0.1015051609795656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.721317988728301E-4	0.0	0.0	0.32791881764176023	0.0
2	2.721317988728301E-4	0.0	0.0	0.45609289491086324	0.0
3	2.721317988728301E-4	0.0	0.0	0.6196441060334341	0.0
4	2.721317988728301E-4	0.0	0.0	1.398213182608601	0.0
5	2.721317988728301E-4	0.0	0.0	1.518223305911519	0.0
6	2.721317988728301E-4	0.0	0.0	2.230936487159461	0.0
7	2.721317988728301E-4	0.0	0.0	2.768668921732173	0.0
8	2.721317988728301E-4	0.0	0.0	3.2424503835697704	0.0
9	2.721317988728301E-4	0.0	0.0	3.976661976928666	0.0
10	2.721317988728301E-4	0.0	0.0	5.028451379572155	0.0
11	2.721317988728301E-4	0.0	0.0	5.928391238444603	0.0
12	2.721317988728301E-4	0.0	0.0	6.362985721244513	0.0
13	2.721317988728301E-4	0.0	0.0	6.532795963741159	0.0
14	2.721317988728301E-4	0.0	0.0	6.74152105347662	0.0
15	2.721317988728301E-4	0.0	0.0	6.930108390095491	0.0
16	2.721317988728301E-4	0.0	0.0	7.273538720273002	0.0
17	2.721317988728301E-4	0.0	0.0	7.686906922760832	0.0
18	2.721317988728301E-4	0.0	0.0	8.207495054004555	0.0
19	2.721317988728301E-4	0.0	0.0	8.43581363325886	0.0
20	2.721317988728301E-4	0.0	0.0	8.677466670657934	0.0
21	2.721317988728301E-4	0.0	0.0	8.961300136882295	0.0
22	2.721317988728301E-4	0.0	0.0	9.253025425273968	0.0
23	2.721317988728301E-4	0.0	0.0	9.549921217844226	0.0
24	2.721317988728301E-4	0.0	0.0	9.78286603767937	0.0
25	2.721317988728301E-4	0.0	0.0	10.010640353335928	0.0
26	2.721317988728301E-4	0.0	0.0	10.215283466088296	0.0
27	2.721317988728301E-4	0.0	0.0	10.421831501432774	0.0
28	2.721317988728301E-4	0.0	0.0	10.632461513760344	0.0
29	2.721317988728301E-4	0.0	0.0	10.854793193439447	0.0
30	2.721317988728301E-4	0.0	0.0	11.13590534167508	0.0
31	2.721317988728301E-4	0.0	0.0	11.368305897912476	0.0
32	2.721317988728301E-4	0.0	0.0	11.60125071774762	0.0
33	2.721317988728301E-4	0.0	0.0	11.84453654593993	0.0
34	2.721317988728301E-4	0.0	0.0	12.094897800902933	0.0
35	2.721317988728301E-4	0.0	0.0	12.33600657470426	0.0
36	2.721317988728301E-4	0.0	0.0	12.56187596776871	0.0
37	2.721317988728301E-4	0.0	0.0	12.809243772944113	0.0
38	2.721317988728301E-4	0.0	0.0	13.096070688956075	0.0
39	2.721317988728301E-4	0.0	0.0	13.40711733506772	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	45	3.8380676E-10	45.000004	1
TCCGGAT	25	3.885609E-5	45.0	43
CTTCGAA	20	7.027015E-4	45.0	12
CTAGACG	40	6.7939254E-9	45.0	14
CGAACCG	35	1.2089185E-7	45.0	1
CACGGGT	110	0.0	45.0	4
CAACGAA	25	3.885609E-5	45.0	38
ACGTTAC	25	3.885609E-5	45.0	22
TTAGCGA	20	7.027015E-4	45.0	44
CTATCAT	20	7.027015E-4	45.0	15
AACCACG	25	3.885609E-5	45.0	1
GGTCCGT	25	3.885609E-5	45.0	21
ACCGAAA	20	7.027015E-4	45.0	44
ACTTACC	20	7.027015E-4	45.0	11
TATGCAC	20	7.027015E-4	45.0	32
CGTTCAT	20	7.027015E-4	45.0	39
TAGCCGT	25	3.885609E-5	45.0	44
GCACCGA	25	3.885609E-5	45.0	9
CGTTATT	20	7.027015E-4	45.0	19
ATGTACG	20	7.027015E-4	45.0	1
>>END_MODULE