FastQCFastQC Report
Sat 18 Jun 2016
SRR3554277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361354
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80852.2374181550501726No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16090.4452697354948333No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12960.35865107346258795No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT10330.28586925840034977No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.22000586682311526No Hit
GAACTTGGGATGAAACTCAGGAGTTACCTCCCATGCGAGAAATCTTAGATT6800.1881811187920986No Hit
AATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT4480.12397814885126497No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGT4100.11346214515405946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAT253.885479E-545.00000436
TCCATCG253.885479E-545.00000430
ACACGAC351.2088458E-745.00000426
CTCGTAG207.026859E-445.00000444
ATAGGTT253.885479E-545.00000410
ACGTTTG207.026859E-445.00000422
CATGCGA600.045.00000432
CTATCGG302.161356E-645.0000042
TAGCGTT253.885479E-545.00000410
CGCATAG207.026859E-445.0000041
TAGCGCG207.026859E-445.0000041
GCACGTT207.026859E-445.00000420
CCTACCG302.161356E-645.0000041
TTACTAG253.885479E-545.00000445
CACGATT253.885479E-545.00000418
ATGTCGC207.026859E-445.00000425
TCGATCA351.2088458E-745.00000417
TGTGATC207.026859E-445.00000434
CGTTAAG253.885479E-545.0000041
AAACGTA253.885479E-545.00000419