Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554274_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 527677 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC | 2396 | 0.4540656500093807 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2175 | 0.4121839686020047 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT | 2083 | 0.3947490605048164 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT | 2044 | 0.3873581755505735 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 996 | 0.1887518311391249 | No Hit |
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT | 719 | 0.13625759697693854 | Illumina Single End Adapter 1 (95% over 21bp) |
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 704 | 0.13341494891761438 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT | 546 | 0.10347238935939979 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCCGGAA | 20 | 7.029812E-4 | 45.000004 | 19 |
AACCGTA | 20 | 7.029812E-4 | 45.000004 | 30 |
TCCATCG | 50 | 2.1827873E-11 | 45.000004 | 29 |
ATTAGCG | 20 | 7.029812E-4 | 45.000004 | 1 |
ACGTTAC | 20 | 7.029812E-4 | 45.000004 | 41 |
TTTCGTA | 20 | 7.029812E-4 | 45.000004 | 30 |
CGGCACG | 20 | 7.029812E-4 | 45.000004 | 1 |
ATAGCCG | 20 | 7.029812E-4 | 45.000004 | 1 |
TAACGCG | 30 | 2.1632604E-6 | 45.000004 | 1 |
ATTCCGG | 45 | 3.8380676E-10 | 45.000004 | 2 |
CAGATAG | 25 | 3.887927E-5 | 45.000004 | 1 |
AACGCCG | 20 | 7.029812E-4 | 45.000004 | 29 |
TCATGCG | 25 | 3.887927E-5 | 45.000004 | 1 |
CGAATAG | 30 | 2.1632604E-6 | 45.000004 | 1 |
CATACCG | 25 | 3.887927E-5 | 45.000004 | 1 |
GTATACG | 30 | 2.1632604E-6 | 45.000004 | 1 |
CGCACCG | 40 | 6.8030204E-9 | 45.000004 | 1 |
CGCACAG | 30 | 2.1632604E-6 | 45.000004 | 1 |
CGACTCG | 20 | 7.029812E-4 | 45.000004 | 1 |
TATCGAG | 20 | 7.029812E-4 | 45.000004 | 1 |