FastQCFastQC Report
Sat 18 Jun 2016
SRR3554274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527677
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC23960.4540656500093807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21750.4121839686020047No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT20830.3947490605048164No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT20440.3873581755505735No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9960.1887518311391249No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT7190.13625759697693854Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7040.13341494891761438No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT5460.10347238935939979No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA207.029812E-445.00000419
AACCGTA207.029812E-445.00000430
TCCATCG502.1827873E-1145.00000429
ATTAGCG207.029812E-445.0000041
ACGTTAC207.029812E-445.00000441
TTTCGTA207.029812E-445.00000430
CGGCACG207.029812E-445.0000041
ATAGCCG207.029812E-445.0000041
TAACGCG302.1632604E-645.0000041
ATTCCGG453.8380676E-1045.0000042
CAGATAG253.887927E-545.0000041
AACGCCG207.029812E-445.00000429
TCATGCG253.887927E-545.0000041
CGAATAG302.1632604E-645.0000041
CATACCG253.887927E-545.0000041
GTATACG302.1632604E-645.0000041
CGCACCG406.8030204E-945.0000041
CGCACAG302.1632604E-645.0000041
CGACTCG207.029812E-445.0000041
TATCGAG207.029812E-445.0000041