FastQCFastQC Report
Sat 18 Jun 2016
SRR3554273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514212
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC23720.4612883402176534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20710.40275217225580107No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT20090.3906948884895724No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT19550.3801933832738248No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9610.1868878983765451No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT7860.15285524258477048Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7090.13788087403638966No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT5830.11337736186631195No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG207.029645E-445.0000041
TCGTTCG207.029645E-445.00000445
CGTATGG207.029645E-445.0000042
ATCTACG453.8380676E-1045.0000041
TAGCGAG253.887788E-545.0000041
CAACCCG302.163153E-645.0000041
TCTAGCG351.2101737E-745.0000041
CCATGCG253.887788E-545.0000041
AATATCG207.029645E-445.0000041
GTAATCG253.887788E-545.0000041
TAGCACG253.887788E-545.0000041
TACGCCC302.163153E-645.00000438
ATTCACG302.163153E-645.0000041
CACTACG207.029645E-445.0000041
TTGCGTC207.029645E-445.00000415
CGAATAT207.029645E-445.00000413
GCGATGT207.029645E-445.0000049
TTACGTA207.029645E-445.00000440
ATGCGGT302.163153E-645.00000412
GTATACG253.887788E-545.0000041