##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514212
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.359361508482884	34.0	31.0	34.0	30.0	34.0
2	32.545434567843614	34.0	31.0	34.0	31.0	34.0
3	32.61481062285594	34.0	31.0	34.0	31.0	34.0
4	36.10125979168125	37.0	35.0	37.0	35.0	37.0
5	35.94448009770289	37.0	35.0	37.0	35.0	37.0
6	35.92205160517452	37.0	35.0	37.0	35.0	37.0
7	35.891089667296754	37.0	35.0	37.0	35.0	37.0
8	36.06263564444237	37.0	35.0	37.0	35.0	37.0
9	37.71790817794995	39.0	38.0	39.0	35.0	39.0
10	37.31732437204888	39.0	37.0	39.0	34.0	39.0
11	37.290529975963224	39.0	37.0	39.0	34.0	39.0
12	37.25984029933179	39.0	37.0	39.0	34.0	39.0
13	37.24879816106975	39.0	37.0	39.0	34.0	39.0
14	38.50718380745685	40.0	38.0	41.0	34.0	41.0
15	38.55223720955559	40.0	38.0	41.0	34.0	41.0
16	38.50008556782028	40.0	38.0	41.0	34.0	41.0
17	38.48007825566109	40.0	38.0	41.0	34.0	41.0
18	38.3960020380699	40.0	38.0	41.0	34.0	41.0
19	38.35068415361757	40.0	38.0	41.0	34.0	41.0
20	38.30376381725825	40.0	38.0	41.0	34.0	41.0
21	38.29187183496301	40.0	38.0	41.0	34.0	41.0
22	38.232532496324474	40.0	38.0	41.0	34.0	41.0
23	38.1627713861209	40.0	37.0	41.0	34.0	41.0
24	38.16240188871516	40.0	37.0	41.0	34.0	41.0
25	38.09453299417361	40.0	37.0	41.0	33.0	41.0
26	38.006773470864154	40.0	37.0	41.0	33.0	41.0
27	37.93505791385654	40.0	37.0	41.0	33.0	41.0
28	37.865814100021005	40.0	37.0	41.0	33.0	41.0
29	37.7823932541442	40.0	37.0	41.0	33.0	41.0
30	37.78270052040793	40.0	37.0	41.0	33.0	41.0
31	37.69218921378731	40.0	37.0	41.0	33.0	41.0
32	37.63249593552854	40.0	37.0	41.0	33.0	41.0
33	37.5455998693146	40.0	36.0	41.0	33.0	41.0
34	37.451041593739546	40.0	36.0	41.0	32.0	41.0
35	37.37237170661128	40.0	36.0	41.0	32.0	41.0
36	37.26194254509813	40.0	36.0	41.0	32.0	41.0
37	37.21261658615513	40.0	36.0	41.0	32.0	41.0
38	37.153434770094826	39.0	36.0	41.0	31.0	41.0
39	37.084282358249126	39.0	35.0	41.0	31.0	41.0
40	36.99196440378677	39.0	35.0	41.0	31.0	41.0
41	36.86939044596392	39.0	35.0	41.0	31.0	41.0
42	36.82580336514901	39.0	35.0	41.0	31.0	41.0
43	36.78046992291117	39.0	35.0	41.0	31.0	41.0
44	36.70733666269943	39.0	35.0	41.0	31.0	41.0
45	36.594344356024365	39.0	35.0	41.0	30.0	41.0
46	36.43076202033402	39.0	35.0	41.0	30.0	41.0
47	36.37142462641867	39.0	35.0	40.0	30.0	41.0
48	36.26975255342155	39.0	35.0	40.0	30.0	41.0
49	36.16669194806811	39.0	35.0	40.0	30.0	41.0
50	36.0574490676997	38.0	35.0	40.0	29.0	41.0
51	35.00743467674811	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	3.0
13	5.0
14	6.0
15	24.0
16	39.0
17	55.0
18	109.0
19	233.0
20	526.0
21	878.0
22	1249.0
23	1578.0
24	1966.0
25	2250.0
26	2714.0
27	3365.0
28	4242.0
29	5671.0
30	7361.0
31	9670.0
32	12734.0
33	17336.0
34	26943.0
35	35047.0
36	40836.0
37	60975.0
38	105711.0
39	172584.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.21639323858642	33.97878695946419	21.455353045047566	25.349466756901823
2	22.696475383693883	32.489128997378515	20.26829401103047	24.546101607897132
3	21.19534355479841	29.982769752553423	22.955512512348992	25.86637418029918
4	22.891920064098077	30.806943439670796	19.41242133594704	26.88871516028409
5	19.00791891282195	35.25199723071418	17.704954376794007	28.035129479669862
6	21.53917061445474	36.27569951693076	20.251180446975177	21.933949421639323
7	84.39495772171789	7.599783746781482	4.332843263089932	3.6724152684106945
8	86.01199505262421	5.0339159724004885	3.7490373620218898	5.205051612953413
9	78.25585556151937	10.688976531080566	6.657954306783973	4.397213600616088
10	32.84034600514963	40.875164329109396	13.873266279277807	12.41122338646317
11	24.144905214191812	28.547952984372206	28.868637838090127	18.438503963345855
12	27.37392359571539	24.12915295636819	30.56035253941954	17.936570908496886
13	23.35865362924241	28.033768173438194	28.01412646923837	20.593451728081025
14	19.56430421693776	28.035323951988673	29.658195452459296	22.742176378614268
15	19.214059570760696	31.58405482563612	28.408127387147715	20.79375821645547
16	21.29160735260943	29.110755875008753	29.29044829758932	20.307188474792497
17	20.400146243183745	28.963929274307095	28.80037805418777	21.835546428321393
18	20.938834566287834	29.792381352438298	28.060605353433992	21.20817872783988
19	22.82579947570263	30.139319969195583	27.71425015363313	19.320630401468655
20	24.035417298701702	29.243774941074886	28.115252075019644	18.60555568520377
21	23.33823403576735	28.9544001306854	27.53047381235755	20.176892021189705
22	22.237520711301954	28.81574136737377	27.6292657503131	21.31747217101118
23	21.754840416015185	29.1692920429706	27.948783770118162	21.127083770896053
24	19.899574494566444	30.11559434630075	29.366875918881707	20.617955240251103
25	20.673185378793182	30.53409877638017	27.3953155507845	21.397400294042146
26	22.586404051247342	30.781467565906667	26.0950736272199	20.537054755626084
27	20.39217287811253	30.181909407015006	27.980482758084214	21.44543495678825
28	20.841792879201574	29.057275987335963	29.876198921845464	20.224732211617
29	19.89510163123381	30.092063195724723	28.326449013247455	21.686386159794015
30	20.758364254432024	29.65216681057618	27.992540041850443	21.59692889314135
31	22.371512138962142	29.878338117352378	27.115275411697898	20.634874331987586
32	21.825822812380885	29.821163255622196	27.181201527774533	21.171812404222383
33	21.784400208474324	30.063864709497253	27.067435221270603	21.08429986075782
34	21.1548933124859	29.551819094070147	28.884195623594938	20.409091969849012
35	22.328144811867478	28.721811237388472	28.864359447076303	20.085684503667746
36	21.521473633443016	31.554495033176977	27.983205370547555	18.94082596283245
37	21.355199800860344	31.707544748080558	27.676716996102773	19.260538454956322
38	21.91780821917808	28.794543884623465	29.642248722316864	19.64539917388159
39	22.692585937317684	28.52831128017238	27.702581814504523	21.076520968005415
40	22.196681524351824	29.572238687545216	28.54464695495243	19.68643283315053
41	20.677852714444626	28.483193702208425	30.571826406229334	20.267127177117608
42	20.911025024697985	29.014686549516544	27.930697844468817	22.143590581316655
43	21.59478969763444	27.814597870139163	29.405186965687303	21.185425466539094
44	21.01642902149308	29.10920009645827	27.704137593055005	22.170233288993646
45	22.36898399881761	29.51234121335169	27.534557731052562	20.58411705677814
46	19.700629312423672	30.601775143326098	29.383211593661756	20.314383950588475
47	20.98453556120822	29.459055797997713	29.001851376475074	20.554557264318994
48	20.526553250410338	28.452856020474044	30.260087279176684	20.760503449938934
49	20.366113587391972	29.643415556229723	29.419966861916873	20.57050399446143
50	19.943719710936346	28.993100122128617	29.437858315247407	21.62532185168763
51	20.444096987234836	27.910083778674945	29.517591965959568	22.128227268130654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2543.0
1	2000.5
2	1458.0
3	976.0
4	494.0
5	476.0
6	458.0
7	556.5
8	655.0
9	738.0
10	821.0
11	974.0
12	1127.0
13	1063.5
14	1000.0
15	1263.5
16	1527.0
17	1498.5
18	1470.0
19	1944.0
20	2418.0
21	2325.0
22	2232.0
23	3457.0
24	4682.0
25	4867.0
26	6304.5
27	7557.0
28	9691.0
29	11825.0
30	13470.5
31	15116.0
32	17383.0
33	19650.0
34	21877.0
35	24104.0
36	24973.5
37	25843.0
38	27250.5
39	28658.0
40	29231.0
41	29804.0
42	31228.0
43	32652.0
44	35846.5
45	39041.0
46	39887.5
47	40734.0
48	38923.0
49	37112.0
50	35332.5
51	33553.0
52	30174.0
53	26795.0
54	24496.0
55	22197.0
56	21082.0
57	19967.0
58	18265.5
59	16564.0
60	16093.5
61	15623.0
62	14665.5
63	13708.0
64	11520.0
65	9332.0
66	7715.0
67	6098.0
68	5212.0
69	4326.0
70	3538.0
71	2750.0
72	2552.0
73	2354.0
74	1834.0
75	1032.0
76	750.0
77	606.5
78	463.0
79	342.0
80	221.0
81	145.5
82	70.0
83	57.5
84	45.0
85	37.0
86	29.0
87	19.5
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.133422103263733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65705595598592	17.775968222695244
2	8.160430656608092	3.9387823516067337
3	3.115267717712272	2.255462123886639
4	1.8336390312861697	1.7700793890819497
5	1.2703616653176575	1.5329087146458038
6	0.9778630845186046	1.4159509546732163
7	0.8089378871060143	1.3665707639396225
8	0.7079867294635613	1.3668914068522648
9	0.645010433238161	1.4009678141710995
>10	7.5463478881697075	38.805336293021966
>50	0.9792168312707416	16.211100561321835
>100	0.29142397039467244	9.930054027287273
>500	0.003229074464206897	0.5920626680189527
>1k	0.003229074464206897	1.637864708797412
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC	2372	0.4612883402176534	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2071	0.40275217225580107	No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT	2009	0.3906948884895724	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT	1955	0.3801933832738248	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	961	0.1868878983765451	No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	786	0.15285524258477048	Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	709	0.13788087403638966	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT	583	0.11337736186631195	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20633513025755915	0.0
2	0.0	0.0	0.0	0.268371799957994	0.0
3	0.0	0.0	0.0	0.43717377268519597	0.0
4	0.0	0.0	0.0	1.1938655651754528	0.0
5	0.0	0.0	0.0	1.2872122782043205	0.0
6	0.0	0.0	0.0	1.7236081616142758	0.0
7	0.0	0.0	0.0	2.040598041274805	0.0
8	0.0	0.0	0.0	2.303524616306115	0.0
9	0.0	0.0	0.0	3.054965656188498	0.0
10	0.0	0.0	0.0	3.5327841435050136	0.0
11	0.0	0.0	0.0	4.4538050453898395	0.0
12	0.0	0.0	0.0	4.692811525207502	0.0
13	0.0	0.0	0.0	4.825830591273638	0.0
14	0.0	0.0	0.0	5.005134069216588	0.0
15	0.0	0.0	0.0	5.121039571227431	0.0
16	0.0	0.0	0.0	5.302287772358483	0.0033060294197723894
17	0.0	0.0	0.0	5.502205316095307	0.0033060294197723894
18	1.9447231881014056E-4	0.0	0.0	5.87948161458698	0.0033060294197723894
19	1.9447231881014056E-4	0.0	0.0	6.01891826717385	0.0033060294197723894
20	1.9447231881014056E-4	0.0	0.0	6.173912705265533	0.0033060294197723894
21	1.9447231881014056E-4	0.0	0.0	6.343881511905596	0.0033060294197723894
22	1.9447231881014056E-4	0.0	0.0	6.535436745933584	0.0033060294197723894
23	1.9447231881014056E-4	0.0	0.0	6.721741227353698	0.0033060294197723894
24	1.9447231881014056E-4	0.0	0.0	6.860205518346518	0.0033060294197723894
25	1.9447231881014056E-4	0.0	0.0	6.9860291086166795	0.0033060294197723894
26	1.9447231881014056E-4	0.0	0.0	7.104851695409676	0.0033060294197723894
27	1.9447231881014056E-4	0.0	0.0	7.246233071184648	0.0033060294197723894
28	1.9447231881014056E-4	0.0	0.0	7.376140580149822	0.0033060294197723894
29	1.9447231881014056E-4	0.0	0.0	7.521216929982186	0.0033060294197723894
30	1.9447231881014056E-4	0.0	0.0	7.718800805893289	0.0033060294197723894
31	1.9447231881014056E-4	0.0	0.0	7.877490218042364	0.0033060294197723894
32	1.9447231881014056E-4	0.0	0.0	8.025872597294502	0.0033060294197723894
33	1.9447231881014056E-4	0.0	0.0	8.175616282778309	0.0033060294197723894
34	1.9447231881014056E-4	0.0	0.0	8.332166499420472	0.0033060294197723894
35	1.9447231881014056E-4	0.0	0.0	8.497856915046713	0.0033060294197723894
36	1.9447231881014056E-4	0.0	0.0	8.668214666324396	0.0033060294197723894
37	1.9447231881014056E-4	0.0	0.0	8.837211111370408	0.0033060294197723894
38	1.9447231881014056E-4	0.0	0.0	9.009124641198571	0.0033060294197723894
39	1.9447231881014056E-4	0.0	0.0	9.182982894214838	0.0033060294197723894
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.029645E-4	45.000004	1
TCGTTCG	20	7.029645E-4	45.000004	45
CGTATGG	20	7.029645E-4	45.000004	2
ATCTACG	45	3.8380676E-10	45.000004	1
TAGCGAG	25	3.887788E-5	45.000004	1
CAACCCG	30	2.163153E-6	45.000004	1
TCTAGCG	35	1.2101737E-7	45.000004	1
CCATGCG	25	3.887788E-5	45.000004	1
AATATCG	20	7.029645E-4	45.000004	1
GTAATCG	25	3.887788E-5	45.000004	1
TAGCACG	25	3.887788E-5	45.000004	1
TACGCCC	30	2.163153E-6	45.000004	38
ATTCACG	30	2.163153E-6	45.000004	1
CACTACG	20	7.029645E-4	45.000004	1
TTGCGTC	20	7.029645E-4	45.000004	15
CGAATAT	20	7.029645E-4	45.000004	13
GCGATGT	20	7.029645E-4	45.000004	9
TTACGTA	20	7.029645E-4	45.000004	40
ATGCGGT	30	2.163153E-6	45.000004	12
GTATACG	25	3.887788E-5	45.000004	1
>>END_MODULE