FastQCFastQC Report
Sat 18 Jun 2016
SRR3554269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814126
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33250.4084134396886968No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA18330.2251494240449267No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT13220.16238272699803225TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG351.2113378E-745.01
CGAGTAG207.032072E-445.01
ACCGCAC207.032072E-445.028
AATGCCG253.8897993E-545.01
ACGATAG253.8897993E-545.01
TACGCCC207.032072E-445.026
ATATACG253.8897993E-545.01
CAGTCGA207.032072E-445.031
ATCGGTT502.1827873E-1145.034
TATCGCG502.1827873E-1145.01
TCGAACG253.8897993E-545.01
CCGCACG253.8897993E-545.01
AGTCGAG207.032072E-445.032
CGCGAAG850.042.3529431
ATAGCGG1550.042.096772
CGCCGGT750.042.028
CGCTAGT700.041.78571313
TGCGGGT1200.041.2500044
CAGTACG603.6379788E-1241.2500041
TAACACG556.002665E-1140.9090921