Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554269_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 814126 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3325 | 0.4084134396886968 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1833 | 0.2251494240449267 | No Hit |
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT | 1322 | 0.16238272699803225 | TruSeq Adapter, Index 20 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAACCG | 35 | 1.2113378E-7 | 45.0 | 1 |
CGAGTAG | 20 | 7.032072E-4 | 45.0 | 1 |
ACCGCAC | 20 | 7.032072E-4 | 45.0 | 28 |
AATGCCG | 25 | 3.8897993E-5 | 45.0 | 1 |
ACGATAG | 25 | 3.8897993E-5 | 45.0 | 1 |
TACGCCC | 20 | 7.032072E-4 | 45.0 | 26 |
ATATACG | 25 | 3.8897993E-5 | 45.0 | 1 |
CAGTCGA | 20 | 7.032072E-4 | 45.0 | 31 |
ATCGGTT | 50 | 2.1827873E-11 | 45.0 | 34 |
TATCGCG | 50 | 2.1827873E-11 | 45.0 | 1 |
TCGAACG | 25 | 3.8897993E-5 | 45.0 | 1 |
CCGCACG | 25 | 3.8897993E-5 | 45.0 | 1 |
AGTCGAG | 20 | 7.032072E-4 | 45.0 | 32 |
CGCGAAG | 85 | 0.0 | 42.352943 | 1 |
ATAGCGG | 155 | 0.0 | 42.09677 | 2 |
CGCCGGT | 75 | 0.0 | 42.0 | 28 |
CGCTAGT | 70 | 0.0 | 41.785713 | 13 |
TGCGGGT | 120 | 0.0 | 41.250004 | 4 |
CAGTACG | 60 | 3.6379788E-12 | 41.250004 | 1 |
TAACACG | 55 | 6.002665E-11 | 40.909092 | 1 |