##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814126
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24142700269983	34.0	31.0	34.0	30.0	34.0
2	32.42280924574329	34.0	31.0	34.0	30.0	34.0
3	32.518136013344375	34.0	31.0	34.0	30.0	34.0
4	36.03542572034304	37.0	35.0	37.0	35.0	37.0
5	35.87231337655351	37.0	35.0	37.0	35.0	37.0
6	35.85199342607901	37.0	35.0	37.0	35.0	37.0
7	35.954649525994746	37.0	35.0	37.0	35.0	37.0
8	36.079106919567735	37.0	35.0	37.0	35.0	37.0
9	37.70038175908889	39.0	38.0	39.0	35.0	39.0
10	37.23610595902846	39.0	37.0	39.0	34.0	39.0
11	37.22062432596429	39.0	37.0	39.0	34.0	39.0
12	37.24610441135647	39.0	37.0	39.0	34.0	39.0
13	37.23383849674375	39.0	37.0	39.0	34.0	39.0
14	38.493957937714804	40.0	38.0	41.0	34.0	41.0
15	38.49210318795862	40.0	38.0	41.0	34.0	41.0
16	38.47123049749056	40.0	38.0	41.0	34.0	41.0
17	38.42974674681806	40.0	38.0	41.0	34.0	41.0
18	38.37641593561685	40.0	38.0	41.0	34.0	41.0
19	38.36165163623321	40.0	38.0	41.0	34.0	41.0
20	38.2993897750471	40.0	38.0	41.0	34.0	41.0
21	38.2478265035142	40.0	38.0	41.0	34.0	41.0
22	38.2006089966418	40.0	37.0	41.0	34.0	41.0
23	38.11943114456485	40.0	37.0	41.0	33.0	41.0
24	38.087972869064494	40.0	37.0	41.0	33.0	41.0
25	38.0249347152652	40.0	37.0	41.0	33.0	41.0
26	37.95243856602049	40.0	37.0	41.0	33.0	41.0
27	37.892425742452644	40.0	37.0	41.0	33.0	41.0
28	37.78854747299558	40.0	37.0	41.0	33.0	41.0
29	37.713261829250015	40.0	37.0	41.0	33.0	41.0
30	37.678121077081435	40.0	37.0	41.0	33.0	41.0
31	37.56933938972591	40.0	36.0	41.0	33.0	41.0
32	37.54609483052992	40.0	36.0	41.0	33.0	41.0
33	37.44953115365435	40.0	36.0	41.0	32.0	41.0
34	37.36753647469802	40.0	36.0	41.0	32.0	41.0
35	37.3245603260429	40.0	36.0	41.0	32.0	41.0
36	37.24826869550905	39.0	36.0	41.0	32.0	41.0
37	37.18701650604452	39.0	36.0	41.0	31.0	41.0
38	37.13823904407917	39.0	35.0	41.0	31.0	41.0
39	37.06771924738923	39.0	35.0	41.0	31.0	41.0
40	36.948428130289415	39.0	35.0	41.0	31.0	41.0
41	36.88084645374303	39.0	35.0	41.0	31.0	41.0
42	36.83043165308564	39.0	35.0	41.0	31.0	41.0
43	36.74424352004481	39.0	35.0	41.0	31.0	41.0
44	36.68372708892727	39.0	35.0	41.0	31.0	41.0
45	36.5646865472912	39.0	35.0	41.0	30.0	41.0
46	36.45366319218401	39.0	35.0	40.0	30.0	41.0
47	36.349455735353004	39.0	35.0	40.0	30.0	41.0
48	36.255832635243195	39.0	35.0	40.0	30.0	41.0
49	36.155994280983535	38.0	35.0	40.0	30.0	41.0
50	36.05316498920315	38.0	35.0	40.0	29.0	41.0
51	35.01826007276515	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	6.0
13	7.0
14	15.0
15	29.0
16	46.0
17	98.0
18	188.0
19	320.0
20	579.0
21	897.0
22	1381.0
23	1990.0
24	2693.0
25	3523.0
26	4485.0
27	5869.0
28	7450.0
29	9365.0
30	12497.0
31	15995.0
32	21147.0
33	28289.0
34	41658.0
35	55133.0
36	71602.0
37	102409.0
38	168182.0
39	258149.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.58873687856671	33.00656163787915	20.711781714378365	26.692919769175777
2	21.360084311273685	30.78860029037274	20.957075440410943	26.894239957942627
3	19.90871191928522	31.24368955174015	21.842933403428955	27.004665125545678
4	20.431603953196433	31.539958188290264	20.598777093472016	27.429660765041287
5	18.606824987778307	33.968820551118625	17.985790897232125	29.43856356387095
6	20.264676475140213	36.804376718100144	20.062373637495917	22.868573169263726
7	86.01788912281391	5.756479955191211	4.282629470131159	3.943001451863717
8	87.41951491538164	5.294389320572	3.8681973060681023	3.4178984579782488
9	77.8455669024205	10.998911716368227	6.734092757140786	4.421428624070475
10	30.599931705903018	43.64250742514058	13.477275016397952	12.28028585255845
11	21.61655566828722	28.54103664543326	32.825999906648356	17.016407779631162
12	24.488838337063303	25.018977406445686	31.76830122118689	18.723883035304116
13	22.641826940792946	26.435097269955754	30.363358988657772	20.55971680059352
14	19.0357512227837	29.08812640795159	30.027047410351713	21.849074958912993
15	18.686542377961153	30.5693467595925	30.475749454015716	20.268361408430636
16	21.117738531873446	29.791703004203278	29.673170983361295	19.417387480561977
17	19.700636019485927	30.18881598180134	29.826095714913908	20.284452283798824
18	20.365520816188155	30.142631484561356	28.994529102374816	20.49731859687567
19	20.189135342686512	31.621518045118325	28.259999066483566	19.929347545711597
20	22.022635316891982	29.800424012990618	29.415594146360636	18.761346523756764
21	22.048798343253992	30.128137413619022	27.91079022166102	19.912274021465965
22	21.032616572864644	29.522211549563583	28.661165470701093	20.78400640687068
23	19.56724143437257	30.21080275043421	28.910512623353142	21.311443191840084
24	18.866858446972582	30.08711182298563	30.505720244777834	20.540309485263954
25	19.901956208252777	31.793113105342417	26.831718923115094	21.47321176328971
26	20.178694698363646	32.11984385709337	27.37119315683322	20.330268287709767
27	19.795461636159516	31.00232642121735	27.703082815190772	21.49912912743236
28	19.320105241694773	31.33777818175565	28.879927677042623	20.462188899506955
29	20.390824024782404	29.75694180016361	27.850971471246467	22.00126270380752
30	20.371048216123793	29.9093752072775	28.259753404264202	21.459823172334502
31	19.682088521924122	31.861038708995903	26.92519339758219	21.531679371497777
32	20.68070544362912	29.61408921960483	27.731088308197993	21.97411702856806
33	19.20587230969162	31.388384598944143	27.715980081707254	21.689763009656982
34	18.468148664948668	30.55644949307601	28.653549941900884	22.321851900074435
35	19.1906412520912	29.77991121767392	29.170054758108694	21.85939277212618
36	18.846222820546206	32.670987046231176	27.942480647958668	20.540309485263954
37	18.942645241645643	32.80376747579613	27.251064331565384	21.002522950992844
38	18.856417802649712	30.419492805781907	28.844920810783588	21.879168580784793
39	19.390241805322518	30.259075376538767	28.198337849423798	22.152344968714914
40	20.684881701358265	30.759857810707437	28.44854481984361	20.10671566809069
41	18.28046272935639	30.157739711052095	29.969930944350136	21.591866615241376
42	19.66771728209147	30.7978126235988	27.6894485620162	21.845021532293526
43	18.693298088993597	30.931575702041208	28.421399144604152	21.953727064361043
44	20.186555889383214	30.638746336562157	27.368122379091197	21.806575394963435
45	19.006148925350622	30.401313801549147	28.495957628180406	22.096579644919828
46	17.97768404399319	31.30154300439981	28.38430414948055	22.336468802126454
47	18.358214821784344	31.28004756020567	28.968857400451526	21.39288021755846
48	19.310647246249353	29.547023433719104	29.07670311475128	22.065626205280264
49	18.595156032358627	31.605918494188863	27.629506980491964	22.16941849296055
50	18.046592296524125	30.15368628443263	28.94625647627026	22.853464942772987
51	18.74992323055645	29.428737075096485	28.6406526753844	23.18068701896267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4085.0
1	3278.0
2	2471.0
3	1817.5
4	1164.0
5	1319.0
6	1474.0
7	1750.0
8	2026.0
9	2231.5
10	2437.0
11	3035.5
12	3634.0
13	3794.5
14	3955.0
15	4476.0
16	4997.0
17	4888.5
18	4780.0
19	4864.0
20	4948.0
21	5131.0
22	5314.0
23	6872.5
24	8431.0
25	8853.0
26	10609.5
27	11944.0
28	15201.5
29	18459.0
30	21372.0
31	24285.0
32	27622.5
33	30960.0
34	34427.5
35	37895.0
36	39493.0
37	41091.0
38	43384.5
39	45678.0
40	47613.5
41	49549.0
42	50241.5
43	50934.0
44	52587.5
45	54241.0
46	57192.0
47	60143.0
48	61488.5
49	62834.0
50	60754.5
51	58675.0
52	51865.0
53	45055.0
54	39948.5
55	34842.0
56	31689.0
57	28536.0
58	26343.5
59	24151.0
60	22978.5
61	21806.0
62	19693.5
63	17581.0
64	14933.5
65	12286.0
66	10092.5
67	7899.0
68	6423.5
69	4948.0
70	4303.0
71	3658.0
72	3288.0
73	2918.0
74	2506.5
75	1633.5
76	1172.0
77	959.0
78	746.0
79	511.0
80	276.0
81	268.5
82	261.0
83	188.0
84	115.0
85	86.0
86	57.0
87	38.5
88	20.0
89	14.5
90	9.0
91	10.5
92	12.0
93	8.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.676413245798948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08441408679852	21.39206861021124
2	9.512849641823287	5.646145142317968
3	4.105900184256415	3.6554517184198616
4	2.3737837461410205	2.8178154962656654
5	1.6178601212717736	2.400614276637978
6	1.2351767784713372	2.199336990571804
7	0.9332392483423883	1.9386635513705255
8	0.7405969278305282	1.7582608383094316
9	0.6193112146746385	1.6541041980998046
>10	5.997462526326869	36.748188247212326
>50	0.6335274851323572	12.755369186927734
>100	0.14337727254920354	5.984722632531133
>500	0.0012503831908360773	0.2477503241285493
>1k	0.0012503831908360773	0.8015087869960058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3325	0.4084134396886968	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1833	0.2251494240449267	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1322	0.16238272699803225	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1917393622117461	0.0
2	1.2283110968081107E-4	0.0	0.0	0.288038952201502	0.0
3	1.2283110968081107E-4	0.0	0.0	0.4060796486047614	0.0
4	1.2283110968081107E-4	0.0	0.0	0.8814360430695003	0.0
5	1.2283110968081107E-4	0.0	0.0	0.9794552685947875	0.0
6	1.2283110968081107E-4	0.0	0.0	1.6411464564453169	0.0
7	1.2283110968081107E-4	0.0	0.0	2.1590024148596165	0.0
8	1.2283110968081107E-4	0.0	0.0	2.763208643379526	0.0
9	1.2283110968081107E-4	0.0	0.0	3.4273564534236716	0.0
10	1.2283110968081107E-4	0.0	0.0	4.504093960885662	0.0
11	1.2283110968081107E-4	0.0	0.0	5.307163755978804	0.0
12	1.2283110968081107E-4	0.0	0.0	5.728105968854944	0.0
13	1.2283110968081107E-4	0.0	0.0	5.955957677312848	0.0
14	1.2283110968081107E-4	0.0	0.0	6.1662445370863965	0.0
15	1.2283110968081107E-4	0.0	0.0	6.336856948433043	0.0
16	1.2283110968081107E-4	0.0	0.0	6.640863944893051	0.0
17	1.2283110968081107E-4	0.0	0.0	7.041539024671857	0.0
18	1.2283110968081107E-4	0.0	0.0	7.477220970709694	0.0
19	1.2283110968081107E-4	0.0	0.0	7.706669483593449	0.0
20	1.2283110968081107E-4	0.0	0.0	7.952577365174433	0.0
21	1.2283110968081107E-4	0.0	0.0	8.229561517504662	0.0
22	1.2283110968081107E-4	0.0	0.0	8.569312366881785	0.0
23	1.2283110968081107E-4	0.0	0.0	8.889778732039021	0.0
24	1.2283110968081107E-4	0.0	0.0	9.133844146974793	0.0
25	1.2283110968081107E-4	0.0	0.0	9.355431468838976	0.0
26	1.2283110968081107E-4	0.0	0.0	9.559699604238165	0.0
27	1.2283110968081107E-4	0.0	0.0	9.790499259328408	0.0
28	1.2283110968081107E-4	0.0	0.0	10.0344418431545	0.0
29	3.6849332904243323E-4	0.0	0.0	10.302827817807072	0.0
30	3.6849332904243323E-4	0.0	0.0	10.612607876422077	0.0
31	3.6849332904243323E-4	0.0	0.0	10.869939051203376	0.0
32	3.6849332904243323E-4	0.0	0.0	11.147783021301372	0.0
33	3.6849332904243323E-4	0.0	0.0	11.409904609360222	0.0
34	3.6849332904243323E-4	0.0	0.0	11.666498797483436	0.0
35	3.6849332904243323E-4	0.0	0.0	11.946553727555685	0.0
36	3.6849332904243323E-4	0.0	0.0	12.220344271034213	0.0
37	3.6849332904243323E-4	0.0	0.0	12.48504531239636	0.0
38	3.6849332904243323E-4	0.0	0.0	12.759941335862017	0.0
39	3.6849332904243323E-4	0.0	0.0	13.061614541238088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	35	1.2113378E-7	45.0	1
CGAGTAG	20	7.032072E-4	45.0	1
ACCGCAC	20	7.032072E-4	45.0	28
AATGCCG	25	3.8897993E-5	45.0	1
ACGATAG	25	3.8897993E-5	45.0	1
TACGCCC	20	7.032072E-4	45.0	26
ATATACG	25	3.8897993E-5	45.0	1
CAGTCGA	20	7.032072E-4	45.0	31
ATCGGTT	50	2.1827873E-11	45.0	34
TATCGCG	50	2.1827873E-11	45.0	1
TCGAACG	25	3.8897993E-5	45.0	1
CCGCACG	25	3.8897993E-5	45.0	1
AGTCGAG	20	7.032072E-4	45.0	32
CGCGAAG	85	0.0	42.352943	1
ATAGCGG	155	0.0	42.09677	2
CGCCGGT	75	0.0	42.0	28
CGCTAGT	70	0.0	41.785713	13
TGCGGGT	120	0.0	41.250004	4
CAGTACG	60	3.6379788E-12	41.250004	1
TAACACG	55	6.002665E-11	40.909092	1
>>END_MODULE