##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	802263
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968049131020624	33.0	31.0	34.0	30.0	34.0
2	32.18560123051917	34.0	31.0	34.0	30.0	34.0
3	32.290760012614314	34.0	31.0	34.0	30.0	34.0
4	35.87392039767508	37.0	35.0	37.0	35.0	37.0
5	35.66733726969834	37.0	35.0	37.0	33.0	37.0
6	35.63913330167289	37.0	35.0	37.0	33.0	37.0
7	35.85601729108784	37.0	35.0	37.0	35.0	37.0
8	35.99042458645108	37.0	35.0	37.0	35.0	37.0
9	37.57367347116843	39.0	37.0	39.0	35.0	39.0
10	37.001594240292775	39.0	37.0	39.0	33.0	39.0
11	36.979534142793575	39.0	37.0	39.0	33.0	39.0
12	36.99400321341007	39.0	37.0	39.0	33.0	39.0
13	36.96533804999109	39.0	37.0	39.0	33.0	39.0
14	38.1353022637215	40.0	37.0	41.0	33.0	41.0
15	38.11552446018326	40.0	37.0	41.0	33.0	41.0
16	38.094889082507855	40.0	37.0	41.0	33.0	41.0
17	38.03565040391991	40.0	37.0	41.0	33.0	41.0
18	37.957329454306134	40.0	37.0	41.0	33.0	41.0
19	37.931987390668645	40.0	37.0	41.0	33.0	41.0
20	37.876813463913955	40.0	37.0	41.0	33.0	41.0
21	37.80299851794237	40.0	37.0	41.0	33.0	41.0
22	37.75483226822127	39.0	37.0	41.0	33.0	41.0
23	37.659764939926184	39.0	36.0	41.0	32.0	41.0
24	37.606632737643395	39.0	36.0	41.0	32.0	41.0
25	37.57863319135994	39.0	36.0	41.0	32.0	41.0
26	37.46538229981939	39.0	36.0	41.0	32.0	41.0
27	37.403142111751386	39.0	36.0	41.0	32.0	41.0
28	37.35132493957717	39.0	36.0	41.0	32.0	41.0
29	37.20828830445876	39.0	36.0	41.0	32.0	41.0
30	37.136146126644256	39.0	36.0	41.0	31.0	41.0
31	37.0699197146073	39.0	35.0	41.0	31.0	41.0
32	36.97273462692409	39.0	35.0	41.0	31.0	41.0
33	36.93756660845633	39.0	35.0	41.0	31.0	41.0
34	36.80995882896257	39.0	35.0	41.0	31.0	41.0
35	36.801315777993004	39.0	35.0	41.0	31.0	41.0
36	36.6986910776142	39.0	35.0	40.0	30.0	41.0
37	36.68143738400001	39.0	35.0	40.0	30.0	41.0
38	36.58249975382138	39.0	35.0	40.0	30.0	41.0
39	36.50527819430785	39.0	35.0	40.0	30.0	41.0
40	36.45785733606062	39.0	35.0	40.0	30.0	41.0
41	36.397934343226595	39.0	35.0	40.0	30.0	41.0
42	36.269755678624094	38.0	35.0	40.0	30.0	41.0
43	36.194078500441876	38.0	35.0	40.0	30.0	41.0
44	36.0708022182252	38.0	35.0	40.0	30.0	41.0
45	35.878885103762734	38.0	35.0	40.0	29.0	41.0
46	35.72622444260797	38.0	35.0	40.0	28.0	41.0
47	35.592252166683494	38.0	34.0	40.0	28.0	41.0
48	35.49793646223246	38.0	34.0	40.0	27.0	41.0
49	35.39311672107526	38.0	34.0	40.0	27.0	41.0
50	35.24939203228866	38.0	34.0	40.0	27.0	41.0
51	34.11070808450595	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	12.0
13	17.0
14	27.0
15	35.0
16	65.0
17	101.0
18	188.0
19	428.0
20	703.0
21	1108.0
22	1764.0
23	2417.0
24	3248.0
25	4121.0
26	5468.0
27	6974.0
28	9135.0
29	11573.0
30	15291.0
31	19947.0
32	25992.0
33	34133.0
34	49326.0
35	63633.0
36	79485.0
37	107438.0
38	160121.0
39	199418.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.24820912842796	34.04943266734225	21.073388651851076	27.628969552378706
2	20.747560338691926	31.715908623481326	21.092958294225213	26.443572743601536
3	18.952762373436144	30.93673770322201	22.500227481511672	27.610272441830176
4	18.721167497441613	32.03625245087957	20.900253408171636	28.342326643507178
5	17.33845384867556	34.30658026108645	18.643761459770676	29.711204430467316
6	20.589258136047654	35.40609500874402	20.519206295192475	23.485440560015856
7	85.13766682496887	5.324064552397406	5.282183024768686	4.256085597865039
8	86.7717942869109	4.904002802073634	4.050168087023831	4.2740348239916335
9	77.30980987531521	10.294255125812857	7.977433834041954	4.418501164829987
10	29.119752500115297	44.54087998574034	13.54518406058861	12.794183453555755
11	20.019993443546568	26.847056389238936	35.71509592240948	17.41785424480501
12	23.83245893179668	23.782226028122945	33.096378618981554	19.288936421098818
13	22.58137294129232	25.78244291460531	30.875660475430127	20.760523668672242
14	18.343236569553873	27.736540261734614	31.380233165433282	22.539990003278227
15	17.631375247269286	30.631226916858935	30.864816151311974	20.8725816845598
16	19.209162082758397	30.718604746822425	29.276184991704717	20.79604817871446
17	18.72565480397326	31.029350724139093	30.45086212376739	19.794132348120254
18	19.30638705761078	29.953145040965367	29.24776538367094	21.492702517752907
19	18.48670573116297	33.32074394556398	28.783952394663597	19.408597928609446
20	20.62652770974107	30.96067000472414	29.089837123237643	19.32296516229715
21	19.30775817905101	33.205195802374035	27.669729253374513	19.81731676520044
22	19.02630434159372	30.69255343945813	28.729980068880156	21.551162150067995
23	18.594151793115223	32.147811877152506	27.629592789396995	21.628443540335276
24	17.1676869056656	30.605175609494644	30.391903901837676	21.835233583002083
25	18.325411990830936	32.217115833585744	27.005358591883212	22.452113583700108
26	18.92957795635596	31.84828416616496	27.72644382203841	21.495694055440673
27	16.854323332872138	31.60223019134623	27.969506259169375	23.573940216612257
28	17.985997110673182	31.115731374873327	29.77190771604823	21.12636379840526
29	18.960116570251902	30.8679323364034	27.14558692099723	23.02636417234747
30	18.61970451086489	30.420074215064137	29.991037851677067	20.969183422393904
31	18.96497781899452	31.118348970350123	26.917232877497778	22.99944033315758
32	19.776432416800972	30.400753867497315	27.36534029364435	22.457473422057355
33	18.324414811601685	28.708166773240194	28.964566482562454	24.00285193259567
34	16.44510590666652	29.69699462645043	31.045679534018145	22.812219932864906
35	16.714593593372747	29.434861136559952	30.114563428701064	23.735981841366236
36	16.230587724972985	32.3236893637124	29.79758508120155	21.64813783011307
37	16.666728990368497	31.676644691329404	28.740325803383676	22.916300514918426
38	16.890346432528982	31.549255044792048	30.14809358028477	21.412304942394204
39	16.438499594272702	30.957429172229055	27.848473630218518	24.75559760327972
40	19.54745513628324	30.37083849061966	26.62505936332599	23.45664700977111
41	16.41556447199983	28.81386777154125	29.6964960368358	25.074071719623113
42	17.87057361488689	29.84794263227894	27.714353023883685	24.56713072895048
43	16.99841573149952	29.2163542379494	29.52124178729419	24.263988243256886
44	19.09909842532935	29.56063036684977	27.937845818640522	23.402425389180355
45	18.74023855020112	29.7731541900848	28.584017959197922	22.902589300516166
46	16.50693101888034	31.294600399120988	28.678749985977163	23.519718596021505
47	16.99878967371049	29.900294541814844	28.868712629150288	24.232203155324374
48	17.762005726301723	29.04321899426996	30.40823271171673	22.786542567711585
49	18.29749097241179	28.786071400525763	28.834683887951957	24.081753739110493
50	17.36375727161791	27.861686255006152	29.923105016684055	24.851451456691883
51	17.10237166614938	27.33692068561058	30.055106617156717	25.505601031083323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6198.0
1	4751.5
2	3305.0
3	2261.0
4	1217.0
5	1280.5
6	1344.0
7	1766.5
8	2189.0
9	2689.0
10	3189.0
11	3987.0
12	4785.0
13	5566.0
14	6347.0
15	6064.5
16	5782.0
17	5495.5
18	5209.0
19	5003.5
20	4798.0
21	4635.5
22	4473.0
23	5286.5
24	6100.0
25	7309.5
26	9379.5
27	10240.0
28	12892.5
29	15545.0
30	17390.0
31	19235.0
32	23192.0
33	27149.0
34	30003.0
35	32857.0
36	36997.5
37	41138.0
38	40297.0
39	39456.0
40	40638.0
41	41820.0
42	45747.5
43	49675.0
44	53284.0
45	56893.0
46	63465.5
47	70038.0
48	74520.0
49	79002.0
50	73469.0
51	67936.0
52	59646.0
53	51356.0
54	44252.0
55	37148.0
56	32052.0
57	26956.0
58	23169.0
59	19382.0
60	18225.0
61	17068.0
62	14693.0
63	12318.0
64	10509.5
65	8701.0
66	6816.0
67	4931.0
68	4092.5
69	3254.0
70	2583.0
71	1912.0
72	1699.5
73	1487.0
74	1318.5
75	1070.5
76	991.0
77	778.5
78	566.0
79	438.5
80	311.0
81	211.0
82	111.0
83	98.0
84	85.0
85	63.5
86	42.0
87	33.0
88	24.0
89	15.5
90	7.0
91	7.0
92	7.0
93	7.0
94	7.0
95	5.0
96	3.0
97	4.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	802263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.36362146615116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57773321489346	24.548196393191628
2	10.029250206409696	6.69224214951943
3	4.177209263096161	4.181004859165217
4	2.324453922198011	3.1020880310289924
5	1.5546090199983482	2.593369343554455
6	1.177252679108496	2.356644765347291
7	0.8745231048321148	2.0424080483115334
8	0.6749983264607793	1.801631092345837
9	0.5369453719477545	1.6122997923899955
>10	4.405314533309111	29.521145400112893
>50	0.4824019802439979	11.115965129031952
>100	0.1819323139442941	8.869593628590712
>500	0.0022507090384861076	0.49335315484555564
>1k	7.502363461620358E-4	0.4112527820452805
>5k	3.751181730810179E-4	0.6588054305192808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5264	0.6561439328499507	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	2207	0.2750968198707905	TruSeq Adapter, Index 13 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1079	0.13449454854580106	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	938	0.11691926463017739	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.985896146276221E-4	0.0	0.0	0.34751696139545263	0.0
2	4.985896146276221E-4	0.0	0.0	0.544833801384334	0.0
3	4.985896146276221E-4	0.0	0.0	0.7743096715166972	0.0
4	4.985896146276221E-4	0.0	0.0	1.8886574602094326	0.0
5	4.985896146276221E-4	0.0	0.0	2.0887165430787658	0.0
6	4.985896146276221E-4	0.0	0.0	3.416834629043094	0.0
7	4.985896146276221E-4	0.0	0.0	4.417877927811703	0.0
8	4.985896146276221E-4	0.0	0.0	5.424904301955842	0.0
9	4.985896146276221E-4	0.0	0.0	6.626131330997441	0.0
10	4.985896146276221E-4	0.0	0.0	8.598302551657	0.0
11	4.985896146276221E-4	0.0	0.0	10.109029083978696	0.0
12	4.985896146276221E-4	0.0	0.0	10.925594225335084	0.0
13	4.985896146276221E-4	0.0	0.0	11.296544898618034	0.0
14	4.985896146276221E-4	0.0	0.0	11.679711017459361	0.0
15	4.985896146276221E-4	0.0	0.0	11.979737318061533	0.0
16	4.985896146276221E-4	0.0	0.0	12.541647813746863	0.0
17	4.985896146276221E-4	0.0	0.0	13.286914640211501	0.0
18	4.985896146276221E-4	0.0	0.0	14.166925310029255	0.0
19	4.985896146276221E-4	0.0	0.0	14.626998876926892	0.0
20	4.985896146276221E-4	0.0	0.0	15.056409182524932	0.0
21	4.985896146276221E-4	0.0	0.0	15.56771283232556	0.0
22	4.985896146276221E-4	0.0	0.0	16.13273951310231	0.0
23	4.985896146276221E-4	0.0	0.0	16.673210655358655	0.0
24	4.985896146276221E-4	0.0	0.0	17.09476817452631	0.0
25	4.985896146276221E-4	0.0	0.0	17.461979425699553	0.0
26	4.985896146276221E-4	0.0	0.0	17.840159648394604	0.0
27	4.985896146276221E-4	0.0	0.0	18.234917975776025	0.0
28	4.985896146276221E-4	0.0	0.0	18.62456575960751	0.0
29	4.985896146276221E-4	0.0	0.0	19.049364111270243	0.0
30	4.985896146276221E-4	0.0	0.0	19.61065136993729	0.0
31	4.985896146276221E-4	0.0	0.0	19.994316078393243	0.0
32	4.985896146276221E-4	0.0	0.0	20.4167461293865	0.0
33	4.985896146276221E-4	0.0	0.0	20.81836006396905	0.0
34	4.985896146276221E-4	0.0	0.0	21.222466946624735	0.0
35	4.985896146276221E-4	0.0	0.0	21.634301968307152	0.0
36	6.232370182845277E-4	0.0	0.0	22.038533498366494	0.0
37	6.232370182845277E-4	0.0	0.0	22.436906600453966	0.0
38	6.232370182845277E-4	0.0	0.0	22.833659286293898	0.0
39	6.232370182845277E-4	0.0	0.0	23.271794910147918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.03201E-4	45.000004	1
CGACGTA	20	7.03201E-4	45.000004	15
CGTTGAA	20	7.03201E-4	45.000004	30
TCGATTG	20	7.03201E-4	45.000004	1
TTATCCG	30	2.1646774E-6	45.000004	1
CGCCTAC	20	7.03201E-4	45.000004	20
TCGGAGC	30	2.1646774E-6	45.000004	42
TCGGACG	20	7.03201E-4	45.000004	1
TCGTGGC	20	7.03201E-4	45.000004	32
TAACCCG	40	6.8102963E-9	45.000004	1
CGTAGCG	40	6.8102963E-9	45.000004	1
CGCTAGG	20	7.03201E-4	45.000004	2
GTATACG	20	7.03201E-4	45.000004	1
TTACGAG	30	2.1646774E-6	45.000004	1
CCGAACG	40	6.8102963E-9	45.000004	1
ACGACGT	20	7.03201E-4	45.000004	14
GCATCGT	20	7.03201E-4	45.000004	35
TCAACCG	60	0.0	45.000004	1
CCCGCAA	20	7.03201E-4	45.000004	16
ACGCGGT	20	7.03201E-4	45.000004	3
>>END_MODULE