##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228531
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.017542477825764	33.0	31.0	34.0	30.0	34.0
2	32.22624939286136	34.0	31.0	34.0	30.0	34.0
3	32.33779224700369	34.0	31.0	34.0	30.0	34.0
4	35.908830749438806	37.0	35.0	37.0	35.0	37.0
5	35.692965068196436	37.0	35.0	37.0	33.0	37.0
6	35.66730552966556	37.0	35.0	37.0	33.0	37.0
7	35.70383886649951	37.0	35.0	37.0	35.0	37.0
8	35.85270269678949	37.0	35.0	37.0	35.0	37.0
9	37.488577042064314	39.0	37.0	39.0	35.0	39.0
10	37.01984851070533	39.0	37.0	39.0	33.0	39.0
11	36.98134607558712	39.0	37.0	39.0	33.0	39.0
12	37.0279830745063	39.0	37.0	39.0	33.0	39.0
13	37.01126761795992	39.0	37.0	39.0	33.0	39.0
14	38.20798928810533	40.0	38.0	41.0	33.0	41.0
15	38.241555850191006	40.0	38.0	41.0	33.0	41.0
16	38.20936765690431	40.0	38.0	41.0	33.0	41.0
17	38.12399193107281	40.0	37.0	41.0	33.0	41.0
18	37.981967435490155	40.0	37.0	41.0	33.0	41.0
19	37.87011827717028	40.0	36.0	41.0	33.0	41.0
20	37.78724986982073	39.0	36.0	41.0	33.0	41.0
21	37.69107035806958	39.0	36.0	41.0	33.0	41.0
22	37.655836626103245	39.0	36.0	41.0	33.0	41.0
23	37.52259430886838	39.0	36.0	41.0	33.0	41.0
24	37.47962858430585	39.0	36.0	41.0	32.0	41.0
25	37.38854684922396	39.0	36.0	41.0	32.0	41.0
26	37.27421225129195	39.0	35.0	41.0	32.0	41.0
27	37.19771934660943	39.0	35.0	41.0	32.0	41.0
28	37.14692536242348	39.0	35.0	41.0	32.0	41.0
29	36.98121480236817	39.0	35.0	41.0	31.0	41.0
30	36.908091243638715	39.0	35.0	41.0	31.0	41.0
31	36.80492362086544	39.0	35.0	40.0	31.0	41.0
32	36.70235547912537	39.0	35.0	40.0	31.0	41.0
33	36.60216775842227	39.0	35.0	40.0	31.0	41.0
34	36.415917315375154	39.0	35.0	40.0	30.0	41.0
35	36.38054355864193	38.0	35.0	40.0	30.0	41.0
36	36.252871601664545	38.0	35.0	40.0	30.0	41.0
37	36.21049223081332	38.0	35.0	40.0	30.0	41.0
38	36.161085366974284	38.0	35.0	40.0	30.0	41.0
39	36.06986360712551	38.0	35.0	40.0	30.0	41.0
40	35.99667878756055	38.0	35.0	40.0	30.0	41.0
41	35.919428873982085	38.0	35.0	40.0	30.0	41.0
42	35.75415589132328	38.0	35.0	40.0	29.0	41.0
43	35.73180006213599	38.0	35.0	40.0	29.0	41.0
44	35.63788282552476	38.0	35.0	40.0	28.0	41.0
45	35.47952794150466	38.0	34.0	40.0	27.0	41.0
46	35.3655740359076	38.0	34.0	40.0	27.0	41.0
47	35.25739177617041	38.0	34.0	40.0	27.0	41.0
48	35.16676074580691	38.0	34.0	40.0	26.0	41.0
49	35.009132240265	37.0	34.0	40.0	26.0	41.0
50	34.85335031133632	37.0	33.0	40.0	26.0	41.0
51	33.88966048369805	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	6.0
13	7.0
14	8.0
15	7.0
16	22.0
17	46.0
18	106.0
19	177.0
20	297.0
21	504.0
22	721.0
23	990.0
24	1229.0
25	1415.0
26	1676.0
27	2139.0
28	2665.0
29	3351.0
30	4388.0
31	5582.0
32	7347.0
33	9898.0
34	13926.0
35	21634.0
36	23919.0
37	30571.0
38	43171.0
39	52698.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.333753407633974	35.65074322520796	20.889507331609277	26.125996035548788
2	18.705996123064267	35.6831239525491	21.49380171617855	24.117078208208078
3	19.315979013788066	36.36837015547125	21.17524537152509	23.1404054592156
4	18.646048019743493	36.69130227408973	18.92215935693626	25.740490349230523
5	16.938621018592663	38.42410876423767	17.493469157357207	27.143801059812457
6	19.32604329390761	41.12264856846555	18.156398913057746	21.394909224569094
7	81.76571231036489	9.896250399289375	4.353895095194963	3.9841421951507674
8	82.62642704928435	9.558003071793324	3.732535192162114	4.083034686760221
9	74.10285694282176	15.357653885030913	6.852899606617921	3.686589565529403
10	26.12512088075578	51.24906467831498	11.751578560457881	10.874235880471359
11	17.270304685141184	31.382613299727392	35.5509755788055	15.796106436325925
12	21.244382600172408	29.41920352162289	33.11323190289283	16.223181975311885
13	20.50575195487702	31.103001343362607	30.87414836499206	17.517098336768317
14	17.27161741733069	33.321081166231274	30.559530216907117	18.847771199530918
15	15.69721394471647	35.447269735834524	30.086946628684945	18.768569690764053
16	17.593674381156166	35.99380390406553	27.806730815512992	18.60579089926531
17	16.855918890653783	36.17321063663135	28.66525766744993	18.305612805264932
18	17.739387654191336	33.66151638070984	29.675623875973063	18.923472089125763
19	16.452472531078936	37.68635327373529	29.285742415689775	16.575431779495997
20	17.974366716112915	36.48389058814778	28.430278605528354	17.111464090210955
21	16.712393504601124	38.00928539235377	27.447917350381353	17.830403752663752
22	16.834040020828684	34.99043893388643	28.38564571108515	19.789875334199735
23	16.69357767655154	36.740310942497956	27.952444088548162	18.613667292402344
24	15.005404080846798	36.76131465753005	28.59174466483759	19.641536596785556
25	15.43773054859078	38.766731865698745	26.409108611085585	19.386428974624888
26	17.298747215913814	39.05947114395858	25.78818628544924	17.853595354678358
27	15.892811040952868	37.3179131058806	26.28571178527202	20.50356406789451
28	16.784156197627457	36.83526523753889	27.497801173582577	18.882777391251075
29	16.736022684012234	37.130629980177744	25.713798128043898	20.419549207766124
30	17.677689241284554	36.85495622038148	25.58515037347231	19.882204164861662
31	17.07514516630129	39.30407690860321	23.674249882948047	19.946528042147456
32	17.120215638140994	37.90557954938279	24.635607423062954	20.338597389413252
33	16.727271136082194	36.51539616069592	24.965540780025467	21.791791923196417
34	15.994766574337834	37.61546573550197	25.375988377944346	21.01377931221585
35	15.890185576573856	37.254901960784316	26.561823122464784	20.293089340177044
36	16.638005347195786	39.49661096306409	25.30816388148654	18.557219808253585
37	16.420091803737787	38.26264270492843	24.65836144768106	20.658904043652722
38	16.348329110711457	36.20821682835151	27.462794981862416	19.980659079074613
39	15.487176794395507	37.20895633415161	25.492821542810383	21.811045328642503
40	18.242601660168642	37.407178894767014	24.94672495197588	19.403494493088466
41	16.14835624051004	35.83933908310033	28.570740949805497	19.44156372658414
42	17.26242829200415	36.871146584052056	27.13855013105443	18.72787499288937
43	16.383772879828122	34.852164476591796	28.43640468907938	20.3276579545007
44	18.634671007434438	35.1790347917788	25.321728780778102	20.864565420008663
45	17.93848536959975	35.775890360607534	26.626584577147085	19.659039692645635
46	15.21281576678875	36.476451772407245	28.787341761073993	19.523390699730015
47	16.166734491163123	36.32855061238956	26.934639064284493	20.57007583216281
48	17.18935286678831	34.04352144785609	30.012120893883104	18.75500479147249
49	17.655810371459452	33.97700968358778	27.283388249296593	21.08379169565617
50	16.532986772035304	33.58581549111499	26.910572307476887	22.97062542937282
51	17.07295727931878	33.41516030647921	28.278439248942156	21.233443165259853
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7584.0
1	5533.5
2	3483.0
3	2232.5
4	982.0
5	892.5
6	803.0
7	934.5
8	1066.0
9	1466.0
10	1866.0
11	2254.0
12	2642.0
13	2844.5
14	3047.0
15	2906.5
16	2766.0
17	2663.0
18	2560.0
19	2367.5
20	2175.0
21	2199.5
22	2224.0
23	2314.0
24	2404.0
25	2539.5
26	3369.5
27	4064.0
28	4936.0
29	5808.0
30	6297.0
31	6786.0
32	7987.0
33	9188.0
34	9367.0
35	9546.0
36	10153.0
37	10760.0
38	11278.5
39	11797.0
40	11567.0
41	11337.0
42	12152.0
43	12967.0
44	13944.0
45	14921.0
46	16396.0
47	17871.0
48	18399.5
49	18928.0
50	17986.5
51	17045.0
52	14627.5
53	12210.0
54	10265.5
55	8321.0
56	7124.5
57	5928.0
58	5068.0
59	4208.0
60	3710.0
61	3212.0
62	2882.5
63	2553.0
64	2094.5
65	1636.0
66	1351.0
67	1066.0
68	878.0
69	690.0
70	567.5
71	445.0
72	397.5
73	350.0
74	290.5
75	203.0
76	175.0
77	139.5
78	104.0
79	83.5
80	63.0
81	36.5
82	10.0
83	9.5
84	9.0
85	7.0
86	5.0
87	9.0
88	13.0
89	9.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	228531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.529398637383984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69147470681952	27.649640530168774
2	9.248811106718895	6.757070156783981
3	3.834405433571711	4.202055738608766
4	2.267581844970712	3.3133360463131916
5	1.5883853811046824	2.9011381388083017
6	1.1295983517207508	2.4758129094083516
7	0.7882033037457625	2.015481488288241
8	0.6228962278841893	1.8203219694483463
9	0.5162851427270876	1.6973627210312825
>10	3.8128436410680275	27.16655508443056
>50	0.3833207556210395	9.597822614875005
>100	0.11020471724104886	5.7257002332287525
>500	0.0011978773613157486	0.2511694255921516
>1k	0.0035936320839472457	1.455819998162175
>5k	0.0011978773613157486	2.970712944852121
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6789	2.970712944852121	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1187	0.5194043696478814	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	1087	0.4756466299976808	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.4607689985166126	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.2511694255921516	No Hit
ATAGCAGGGATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTT	442	0.19340920925388677	No Hit
CTGTCAGGGATTATATCTATTTTTAAGAAAGTACAATGCTGGTTGTCTGGT	372	0.16277879149874636	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT	309	0.13521141551911994	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC	252	0.1102695039185056	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT	243	0.10633130734998752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.375773965020063E-4	0.0	0.0	0.5863537113126884	0.0
2	4.375773965020063E-4	0.0	0.0	0.8068927191496996	0.0
3	4.375773965020063E-4	0.0	0.0	1.0331202331412368	0.0
4	4.375773965020063E-4	0.0	0.0	2.1463171298423407	0.0
5	4.375773965020063E-4	0.0	0.0	2.3117213857200993	0.0
6	4.375773965020063E-4	0.0	0.0	3.3435288866718302	0.0
7	4.375773965020063E-4	0.0	0.0	4.1819271783696745	0.0
8	4.375773965020063E-4	0.0	0.0	5.067146251493233	0.0
9	4.375773965020063E-4	0.0	0.0	6.225851197430545	0.0
10	4.375773965020063E-4	0.0	0.0	8.045298012085889	0.0
11	4.375773965020063E-4	0.0	0.0	9.470925169889425	0.0
12	4.375773965020063E-4	0.0	0.0	10.176737510447161	0.0
13	4.375773965020063E-4	0.0	0.0	10.497481742083131	0.0
14	4.375773965020063E-4	0.0	0.0	10.800722877859021	0.0
15	4.375773965020063E-4	0.0	0.0	11.079897256827302	0.0
16	4.375773965020063E-4	0.0	0.0	11.579173066236091	0.0
17	4.375773965020063E-4	0.0	0.0	12.270545352709261	0.0
18	4.375773965020063E-4	0.0	0.0	13.10588060263159	0.0
19	4.375773965020063E-4	0.0	0.0	13.477821389658295	0.0
20	4.375773965020063E-4	0.0	0.0	13.856763415029032	0.0
21	4.375773965020063E-4	0.0	0.0	14.28383895401499	0.0
22	4.375773965020063E-4	0.0	0.0	14.742420065549094	0.0
23	4.375773965020063E-4	0.0	0.0	15.193999938739164	0.0
24	4.375773965020063E-4	0.0	0.0	15.53093453404571	0.0
25	4.375773965020063E-4	0.0	0.0	15.817547728754523	0.0
26	4.375773965020063E-4	0.0	0.0	16.088845714585766	0.0
27	4.375773965020063E-4	0.0	0.0	16.403901440067212	0.0
28	4.375773965020063E-4	0.0	0.0	16.725520826496187	0.0
29	4.375773965020063E-4	0.0	0.0	17.04889052251117	0.0
30	4.375773965020063E-4	0.0	0.0	17.506596479252266	0.0
31	4.375773965020063E-4	0.0	0.0	17.815963698579186	0.0
32	4.375773965020063E-4	0.0	0.0	18.092950190564956	0.0
33	4.375773965020063E-4	0.0	0.0	18.427696898888993	0.0
34	4.375773965020063E-4	0.0	0.0	18.75938056543751	0.0
35	4.375773965020063E-4	0.0	0.0	19.12519526891319	0.0
36	4.375773965020063E-4	0.0	0.0	19.477007495700803	0.0
37	4.375773965020063E-4	0.0	0.0	19.837571270418454	0.0
38	4.375773965020063E-4	0.0	0.0	20.153939728089405	0.0
39	4.375773965020063E-4	0.0	0.0	20.49349978777496	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.8809667E-5	45.000004	21
GCCTTAG	25	3.8809667E-5	45.000004	32
ACACGTG	25	3.8809667E-5	45.000004	42
AGTCCAC	25	3.8809667E-5	45.000004	28
CAAACCG	25	3.8809667E-5	45.000004	1
CAAGTTC	25	3.8809667E-5	45.000004	27
CTCGTCC	50	2.1827873E-11	45.000004	37
GCAACAG	35	1.2062264E-7	45.000004	1
TATGGGC	25	3.8809667E-5	45.000004	4
GGTCCTA	25	3.8809667E-5	45.000004	8
TGTATCG	25	3.8809667E-5	45.000004	29
TTCAACT	50	2.1827873E-11	45.000004	23
GGGAGCG	25	3.8809667E-5	45.000004	7
AAAGTCG	25	3.8809667E-5	45.000004	1
AATGCGG	25	3.8809667E-5	45.000004	2
TCGGATC	25	3.8809667E-5	45.000004	40
TCGTGGG	25	3.8809667E-5	45.000004	3
AGCATCG	25	3.8809667E-5	45.000004	1
TACGAGG	25	3.8809667E-5	45.000004	2
TAACACG	25	3.8809667E-5	45.000004	1
>>END_MODULE