FastQCFastQC Report
Sat 18 Jun 2016
SRR3554263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223499
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64762.897552114327133No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.5217025579532795No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT10470.4684584718499859No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10040.44921901216560256No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.28635474879082234No Hit
ATAGCAGGGATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTT4260.1906048796638911No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT3240.1449670915753538No Hit
CTGTCAGGGATTATATCTATTTTTAAGAAAGTACAATGCTGGTTGTCTGGT3230.14451966228036814No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC2550.11409447022134327No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT2550.11409447022134327No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.11409447022134327No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC2270.1015664499617448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA302.1576288E-645.0000045
ACTGCGG351.2060627E-745.0000042
CGAAAAT302.1576288E-645.00000424
CGAAAAG302.1576288E-645.0000041
TGGACGA302.1576288E-645.00000413
TAAACGG351.2060627E-745.0000042
TTCCGTT302.1576288E-645.00000414
CCCACGA351.2060627E-745.00000439
AAAGTCG351.2060627E-745.0000041
GGGACGC302.1576288E-645.0000047
TGCGCGG351.2060627E-745.0000042
CGGGCTA302.1576288E-645.0000046
TAGGGCC302.1576288E-645.0000045
TCCGTTA302.1576288E-645.00000415
GGACGAA302.1576288E-645.00000414
GACGAAC302.1576288E-645.00000415
TTCTGCG351.2060627E-745.0000041
TCTGGGC351.2060627E-745.0000044
GTAACGG302.1576288E-645.0000042
TAGTACG351.2060627E-745.0000041
Produced by FastQC (version 0.11.5)