##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223499
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09880581121168	33.0	31.0	34.0	30.0	34.0
2	32.30152260189084	34.0	31.0	34.0	30.0	34.0
3	32.4056483474199	34.0	31.0	34.0	30.0	34.0
4	35.95130626982671	37.0	35.0	37.0	35.0	37.0
5	35.762602964666506	37.0	35.0	37.0	35.0	37.0
6	35.74220466310811	37.0	35.0	37.0	33.0	37.0
7	35.74849999328856	37.0	35.0	37.0	35.0	37.0
8	35.89813824670357	37.0	35.0	37.0	35.0	37.0
9	37.527680213334285	39.0	37.0	39.0	35.0	39.0
10	37.08241200184341	39.0	37.0	39.0	33.0	39.0
11	37.068224018899414	39.0	37.0	39.0	33.0	39.0
12	37.13851068684871	39.0	37.0	39.0	33.0	39.0
13	37.11757994442928	39.0	37.0	39.0	33.0	39.0
14	38.346252108510555	40.0	38.0	41.0	34.0	41.0
15	38.36516047051665	40.0	38.0	41.0	34.0	41.0
16	38.355053042742924	40.0	38.0	41.0	34.0	41.0
17	38.24210399151674	40.0	38.0	41.0	33.0	41.0
18	38.128380887610234	40.0	37.0	41.0	33.0	41.0
19	37.99834451160855	40.0	37.0	41.0	33.0	41.0
20	37.9297670235661	40.0	37.0	41.0	33.0	41.0
21	37.82484932818491	40.0	36.0	41.0	33.0	41.0
22	37.80253155495103	39.0	36.0	41.0	33.0	41.0
23	37.65593582074193	39.0	36.0	41.0	33.0	41.0
24	37.64311697144059	39.0	36.0	41.0	33.0	41.0
25	37.54700021029177	39.0	36.0	41.0	33.0	41.0
26	37.47650325057383	39.0	36.0	41.0	33.0	41.0
27	37.4368162721086	39.0	36.0	41.0	33.0	41.0
28	37.33898138246703	39.0	35.0	41.0	32.0	41.0
29	37.22812182604844	39.0	35.0	41.0	32.0	41.0
30	37.14139213150842	39.0	35.0	41.0	32.0	41.0
31	36.994518991136424	39.0	35.0	41.0	31.0	41.0
32	36.88868853999347	39.0	35.0	41.0	31.0	41.0
33	36.81840634633712	39.0	35.0	41.0	31.0	41.0
34	36.653121490476465	39.0	35.0	41.0	31.0	41.0
35	36.56159087960125	39.0	35.0	40.0	31.0	41.0
36	36.46976049109839	39.0	35.0	40.0	30.0	41.0
37	36.42429272614195	39.0	35.0	40.0	30.0	41.0
38	36.37576901910076	39.0	35.0	40.0	30.0	41.0
39	36.31266806562893	38.0	35.0	40.0	30.0	41.0
40	36.19239459684383	38.0	35.0	40.0	30.0	41.0
41	36.12413030930787	38.0	35.0	40.0	30.0	41.0
42	35.993995498861295	38.0	35.0	40.0	30.0	41.0
43	35.94046953230216	38.0	35.0	40.0	30.0	41.0
44	35.87599049660177	38.0	35.0	40.0	30.0	41.0
45	35.72447751444078	38.0	35.0	40.0	29.0	41.0
46	35.53171155128211	38.0	35.0	40.0	28.0	41.0
47	35.48728182229003	38.0	34.0	40.0	28.0	41.0
48	35.41717412605873	38.0	34.0	40.0	27.0	41.0
49	35.321630074407494	38.0	34.0	40.0	27.0	41.0
50	35.21117320435438	38.0	34.0	40.0	27.0	41.0
51	34.250041387209784	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	4.0
12	8.0
13	4.0
14	0.0
15	8.0
16	19.0
17	49.0
18	98.0
19	146.0
20	260.0
21	412.0
22	624.0
23	839.0
24	1115.0
25	1314.0
26	1542.0
27	1811.0
28	2356.0
29	3096.0
30	3873.0
31	5134.0
32	6714.0
33	8866.0
34	12919.0
35	20901.0
36	23216.0
37	29552.0
38	42855.0
39	55736.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.073454467357795	35.69769887113589	21.081973521134323	26.14687314037199
2	18.877936814034964	35.35138859681699	21.443943820777722	24.32673076837033
3	19.285544901766897	36.31067700526624	21.124926733452945	23.278851359513915
4	18.683305070716198	36.5178367688446	18.800531546002443	25.99832661443675
5	16.823788920755796	38.5585617832742	17.540570651322827	27.07707864464718
6	19.511496695734657	40.97512740549175	18.03766459805189	21.475711300721702
7	81.7972339920984	9.68863395361948	4.411205419263621	4.102926635018502
8	82.6052912988425	9.409438073548428	3.746772916209916	4.238497711399156
9	73.95872017324461	15.432731242645382	6.92575805708303	3.6827905270269667
10	25.975507720392482	51.123271245061495	11.932044438677577	10.969176595868438
11	17.2667439227916	31.22340592127929	35.720070335885175	15.789779820043936
12	21.389804876084455	29.07887731041302	33.34511563810129	16.18620217540123
13	20.690472888021873	30.917811712804085	30.71020451993074	17.681510879243305
14	17.275692508691314	32.9983579344874	30.755842308019275	18.970107248802005
15	15.646155016353541	35.47353679434807	30.04398229969709	18.836325889601298
16	17.573232989856777	35.65743023458718	28.115562038308894	18.653774737247147
17	16.90074675949333	36.160788191446045	28.52227526745086	18.416189781609763
18	17.634978232564798	33.6225218009924	29.80102819251988	18.941471773922927
19	16.504771833431022	37.85341321437679	29.079324739708007	16.56249021248417
20	18.093145830630114	36.29904384359662	28.50482552494642	17.10298480082685
21	16.715958460664254	37.916053315674795	27.4820021566092	17.885986067051753
22	16.88150729980895	34.95093937780482	28.536593004890403	19.630960317495827
23	16.496718106121282	36.81179781565018	28.00594186103741	18.685542217191127
24	15.083736392556565	36.58763573886237	28.747332202828645	19.581295665752418
25	15.592463500955262	38.53484803063996	26.384010666714392	19.488677801690386
26	17.221106134703064	38.944693264846826	25.979534584047354	17.85466601640276
27	15.855104497111844	37.19032299920805	26.35134832818044	20.603224175499665
28	16.70566758687958	36.65251298663529	27.734352278981113	18.907467147504015
29	16.825578637935738	36.92633971516651	25.69586441102645	20.5522172358713
30	17.685985172193163	36.70351992626365	25.63456659761341	19.97592830392977
31	16.97412516387098	39.33708875654925	23.634110219732527	20.054675859847247
32	17.075244184537738	37.776455375639266	24.63143011825556	20.516870321567435
33	16.849739819864965	36.4399840715171	24.968344377379765	21.74193173123817
34	15.924456037834622	37.33663237866836	25.575058501380322	21.1638530821167
35	15.791569537223879	37.08965140783628	26.643519657806074	20.47525939713377
36	16.377701913655095	39.47847641376471	25.45112058666929	18.692701085910898
37	16.428261423988474	37.96169110376333	24.863198493058132	20.746848979190062
38	16.25152685246914	36.21358484825436	27.588042899520804	19.946845399755702
39	15.462261576114432	37.1232086049602	25.703470709041206	21.71105910988416
40	18.297621018438562	37.50128635922309	24.948657488400393	19.252435133937958
41	16.15040783180238	35.7348355026197	28.781784258542544	19.332972407035378
42	17.300748549210514	36.774661184166376	27.18983082698357	18.73475943963955
43	16.18843932187616	34.6520566087544	28.524959843220778	20.634544226148662
44	18.677935919176374	35.09366932290525	25.27886030809981	20.949534449818568
45	17.939230153155048	35.89635747810952	26.517792025915103	19.646620342820327
46	15.282842428825186	36.4368520664522	28.78849569796733	19.491809806755285
47	16.175463872321576	36.16884191875579	27.07931579112211	20.576378417800527
48	17.261374771251774	33.76346202891288	30.181790522552674	18.793372677282672
49	17.678378874178406	33.9406440297272	27.184909104738725	21.196067991355665
50	16.470767207012113	33.56838285629913	26.96164188654088	22.999208050147875
51	17.06182130568817	33.253840061924215	28.472610615707456	21.211728016680166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7321.0
1	5347.0
2	3373.0
3	2122.5
4	872.0
5	809.5
6	747.0
7	912.5
8	1078.0
9	1444.5
10	1811.0
11	2181.0
12	2551.0
13	2799.5
14	3048.0
15	2842.5
16	2637.0
17	2508.5
18	2380.0
19	2219.5
20	2059.0
21	2162.0
22	2265.0
23	2307.5
24	2350.0
25	2496.0
26	3266.5
27	3891.0
28	4856.5
29	5822.0
30	6278.5
31	6735.0
32	7750.0
33	8765.0
34	9035.5
35	9306.0
36	9987.0
37	10668.0
38	11246.0
39	11824.0
40	11418.0
41	11012.0
42	11822.5
43	12633.0
44	13555.0
45	14477.0
46	15998.5
47	17520.0
48	18253.0
49	18986.0
50	17721.5
51	16457.0
52	14194.0
53	11931.0
54	10161.0
55	8391.0
56	7017.0
57	5643.0
58	4891.0
59	4139.0
60	3626.5
61	3114.0
62	2802.5
63	2491.0
64	1988.5
65	1486.0
66	1262.5
67	1039.0
68	844.0
69	649.0
70	556.0
71	463.0
72	385.5
73	308.0
74	272.0
75	201.0
76	166.0
77	144.0
78	122.0
79	92.0
80	62.0
81	37.0
82	12.0
83	8.0
84	4.0
85	4.5
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	223499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.06817032738402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87346797002927	27.005489957449473
2	9.525877040639111	6.871619112389765
3	4.0539869994541755	4.3865968080394095
4	2.3929439785639857	3.452364440109352
5	1.590333945318315	2.86802178085808
6	1.1586364313005508	2.5073937690996377
7	0.8472683967647496	2.13915945932644
8	0.646305760928894	1.8648853015002305
9	0.5334193420334442	1.7315513715945037
>10	3.8728725251823546	27.19430511993342
>50	0.3895201706941894	9.680132797014753
>100	0.10916488860219323	5.67519317759811
>500	0.0012405100977521957	0.28635474879082234
>1k	0.003721530293256587	1.4393800419688678
>5k	0.0012405100977521957	2.897552114327133
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6476	2.897552114327133	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1166	0.5217025579532795	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	1047	0.4684584718499859	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1004	0.44921901216560256	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	640	0.28635474879082234	No Hit
ATAGCAGGGATGAGATGGTTAAAAAGGCCAAAGATAAAAAGATTTTTTTTT	426	0.1906048796638911	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCT	324	0.1449670915753538	No Hit
CTGTCAGGGATTATATCTATTTTTAAGAAAGTACAATGCTGGTTGTCTGGT	323	0.14451966228036814	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC	255	0.11409447022134327	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGT	255	0.11409447022134327	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	255	0.11409447022134327	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	227	0.1015664499617448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5606289066170318	0.0
2	0.0	0.0	0.0	0.797319003664446	0.0
3	0.0	0.0	0.0	1.0228233683372185	0.0
4	0.0	0.0	0.0	2.181217813055092	0.0
5	0.0	0.0	0.0	2.3467666521997863	0.0
6	0.0	0.0	0.0	3.4103060863806998	0.0
7	0.0	0.0	0.0	4.266238327688267	0.0
8	0.0	0.0	0.0	5.198680978438382	0.0
9	0.0	0.0	0.0	6.351706271616428	0.0
10	0.0	0.0	0.0	8.180797229517806	0.0
11	0.0	0.0	0.0	9.671631640410023	0.0
12	0.0	0.0	0.0	10.366489335522754	0.0
13	0.0	0.0	0.0	10.68237441778263	0.0
14	0.0	0.0	0.0	10.988416055552822	0.0
15	0.0	0.0	0.0	11.263137642674016	0.0
16	0.0	0.0	0.0	11.76783788741784	0.0
17	0.0	0.0	0.0	12.440324117781287	0.0
18	0.0	0.0	0.0	13.298940934858768	0.0
19	0.0	0.0	0.0	13.66270095168211	0.0
20	0.0	0.0	0.0	14.073441044478946	0.0
21	0.0	0.0	0.0	14.515053758629792	0.0
22	0.0	0.0	0.0	14.983064801184792	0.0
23	0.0	0.0	0.0	15.430941525465439	0.0
24	0.0	0.0	0.0	15.76069691586987	0.0
25	0.0	0.0	0.0	16.101638038648943	0.0
26	0.0	0.0	0.0	16.382623635899936	0.0
27	0.0	0.0	0.0	16.69134984944004	0.0
28	0.0	0.0	0.0	17.02468467420436	0.0
29	0.0	0.0	0.0	17.355782352493748	0.0
30	0.0	0.0	0.0	17.813502521264077	0.0
31	0.0	0.0	0.0	18.149969351093294	0.0
32	0.0	0.0	0.0	18.461380140403314	0.0
33	0.0	0.0	0.0	18.788003525742845	0.0
34	0.0	0.0	0.0	19.109705188837534	0.0
35	0.0	0.0	0.0	19.47078062989096	0.0
36	0.0	0.0	0.0	19.812616611260005	0.0
37	0.0	0.0	0.0	20.174139481608417	0.0
38	0.0	0.0	0.0	20.526266336762134	0.0
39	0.0	0.0	0.0	20.882867484865702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTA	30	2.1576288E-6	45.000004	5
ACTGCGG	35	1.2060627E-7	45.000004	2
CGAAAAT	30	2.1576288E-6	45.000004	24
CGAAAAG	30	2.1576288E-6	45.000004	1
TGGACGA	30	2.1576288E-6	45.000004	13
TAAACGG	35	1.2060627E-7	45.000004	2
TTCCGTT	30	2.1576288E-6	45.000004	14
CCCACGA	35	1.2060627E-7	45.000004	39
AAAGTCG	35	1.2060627E-7	45.000004	1
GGGACGC	30	2.1576288E-6	45.000004	7
TGCGCGG	35	1.2060627E-7	45.000004	2
CGGGCTA	30	2.1576288E-6	45.000004	6
TAGGGCC	30	2.1576288E-6	45.000004	5
TCCGTTA	30	2.1576288E-6	45.000004	15
GGACGAA	30	2.1576288E-6	45.000004	14
GACGAAC	30	2.1576288E-6	45.000004	15
TTCTGCG	35	1.2060627E-7	45.000004	1
TCTGGGC	35	1.2060627E-7	45.000004	4
GTAACGG	30	2.1576288E-6	45.000004	2
TAGTACG	35	1.2060627E-7	45.000004	1
>>END_MODULE