FastQCFastQC Report
Sat 18 Jun 2016
SRR3554258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588555
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52560.8930346356755103No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT11960.20320955560652787TruSeq Adapter, Index 15 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10540.1790826685696324No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10000.16990765518940457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG351.2105738E-745.0000041
ACTAGCG351.2105738E-745.0000041
GCGATGC453.8380676E-1045.0000049
TCGAGCT351.2105738E-745.00000416
GCTACGA351.2105738E-745.0000049
CGGAACG207.030478E-445.01
ACAGTCG253.8884784E-545.01
CGAACCG207.030478E-445.01
ATCTACG207.030478E-445.01
GATCGCG207.030478E-445.01
CGGCACG207.030478E-445.01
CCTACGG551.8189894E-1245.02
TAACGCG207.030478E-445.01
CCTCGCG207.030478E-445.01
ATCCGTC253.8884784E-545.044
CGGATTA207.030478E-445.042
CGTAACG406.8048394E-945.01
CGCAATG253.8884784E-545.01
CACGTAC207.030478E-445.020
GACGAGT207.030478E-445.09