##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588555
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15731240071021	33.0	31.0	34.0	30.0	34.0
2	32.35436280381612	34.0	31.0	34.0	30.0	34.0
3	32.45321167945222	34.0	31.0	34.0	30.0	34.0
4	35.99822786315637	37.0	35.0	37.0	35.0	37.0
5	35.82514463389148	37.0	35.0	37.0	35.0	37.0
6	35.81702304797343	37.0	35.0	37.0	35.0	37.0
7	36.00195563711123	37.0	35.0	37.0	35.0	37.0
8	36.128973502901175	37.0	35.0	37.0	35.0	37.0
9	37.735521743932175	39.0	38.0	39.0	35.0	39.0
10	37.202290355191955	39.0	37.0	39.0	34.0	39.0
11	37.123079406342654	39.0	37.0	39.0	34.0	39.0
12	37.21301322731096	39.0	37.0	39.0	34.0	39.0
13	37.2276303828869	39.0	37.0	39.0	34.0	39.0
14	38.44529568179694	40.0	38.0	41.0	34.0	41.0
15	38.45461001945443	40.0	38.0	41.0	34.0	41.0
16	38.410095912871355	40.0	38.0	41.0	34.0	41.0
17	38.38960165150241	40.0	38.0	41.0	34.0	41.0
18	38.29727722982559	40.0	38.0	41.0	34.0	41.0
19	38.29197950913679	40.0	38.0	41.0	34.0	41.0
20	38.215577133827765	40.0	37.0	41.0	34.0	41.0
21	38.16488858305511	40.0	37.0	41.0	34.0	41.0
22	38.112677659691954	40.0	37.0	41.0	33.0	41.0
23	38.01872892083153	40.0	37.0	41.0	33.0	41.0
24	37.96388952604259	40.0	37.0	41.0	33.0	41.0
25	37.89033480303455	40.0	37.0	41.0	33.0	41.0
26	37.803678500734854	40.0	37.0	41.0	33.0	41.0
27	37.727369574636185	40.0	37.0	41.0	33.0	41.0
28	37.68089303463567	40.0	37.0	41.0	33.0	41.0
29	37.5662308535311	40.0	36.0	41.0	33.0	41.0
30	37.49543373176679	40.0	36.0	41.0	32.0	41.0
31	37.444174291272695	39.0	36.0	41.0	32.0	41.0
32	37.37312400710214	39.0	36.0	41.0	32.0	41.0
33	37.27323869476939	39.0	36.0	41.0	32.0	41.0
34	37.15540603681899	39.0	35.0	41.0	31.0	41.0
35	37.14649777845741	39.0	35.0	41.0	31.0	41.0
36	37.021578272209055	39.0	35.0	41.0	31.0	41.0
37	36.96687480354427	39.0	35.0	41.0	31.0	41.0
38	36.911581755315986	39.0	35.0	41.0	31.0	41.0
39	36.85150070936446	39.0	35.0	41.0	31.0	41.0
40	36.78868075201128	39.0	35.0	41.0	31.0	41.0
41	36.73457705736932	39.0	35.0	41.0	31.0	41.0
42	36.60970342618787	39.0	35.0	40.0	30.0	41.0
43	36.555358462675535	39.0	35.0	40.0	30.0	41.0
44	36.475343850617186	39.0	35.0	40.0	30.0	41.0
45	36.35708302537571	39.0	35.0	40.0	30.0	41.0
46	36.25923150767558	38.0	35.0	40.0	30.0	41.0
47	36.11785474594558	38.0	35.0	40.0	30.0	41.0
48	36.0127430741392	38.0	35.0	40.0	29.0	41.0
49	35.89753039223182	38.0	35.0	40.0	29.0	41.0
50	35.741093015945836	38.0	34.0	40.0	28.0	41.0
51	34.70923533059782	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	3.0
12	3.0
13	3.0
14	11.0
15	23.0
16	40.0
17	79.0
18	140.0
19	254.0
20	416.0
21	726.0
22	1070.0
23	1450.0
24	2088.0
25	2663.0
26	3330.0
27	4298.0
28	5414.0
29	7082.0
30	9523.0
31	12116.0
32	16229.0
33	21476.0
34	31016.0
35	43891.0
36	55137.0
37	77554.0
38	123453.0
39	168999.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.60275590216717	32.69023285844143	22.306326511540977	25.400684727850415
2	21.70876128823984	31.232595084571535	21.652521854372146	25.406121772816476
3	20.09073068787114	32.59287577201791	22.129452642488808	25.18694089762214
4	19.62892168106634	33.317701829055906	20.707835291519057	26.345541198358692
5	18.662826753659388	35.2196481212461	17.70012997935622	28.41739514573829
6	20.586691133369015	37.0942392809508	19.04970648452566	23.26936310115452
7	88.23049672502995	5.235874302316692	3.474441640968134	3.059187331685229
8	89.46317676342908	5.285657245287187	2.7705142255184305	2.480651765765306
9	80.56800129129817	11.203880690844525	5.3782569173654124	2.8498611004918826
10	30.05241651162593	48.97452234710435	11.455513928180034	9.517547213089687
11	17.831638504472817	28.09898819991335	37.098147157020165	16.97122613859367
12	21.734417344173444	25.79164224244123	35.05364834212605	17.420292071259272
13	22.042969645997402	27.647713467730288	32.65693095802432	17.652385928247995
14	18.939436416307736	30.921664075574924	30.72593045679673	19.41296905132061
15	17.923898361240663	32.3764134193066	31.281528489266087	18.41815973018664
16	20.974590310166423	33.978302792432316	28.030345507216825	17.016761390184435
17	20.783104382767966	31.423401381349237	29.897460730093194	17.896033505789603
18	20.143402060979856	30.779111552871015	30.24798022274894	18.829506163400193
19	19.79407192191044	32.517606680769	29.65992982813841	18.02839156918215
20	21.66067742182124	31.907638198639038	29.228874106922888	17.202810272616833
21	20.721088088623834	32.61462395188215	29.701047480694243	16.963240478799772
22	20.996848212996237	29.863649106710504	29.8108078259466	19.32869485434666
23	19.280271172617685	32.20684557942758	29.27135102072024	19.241532227234497
24	17.695202657355726	32.27973596350384	31.14883061056316	18.87623076857728
25	18.397091180943157	34.90616849742165	28.224889772408694	18.471850549226495
26	20.30209581092676	33.903203608838595	27.083620052501466	18.711080527733177
27	18.589086831307185	32.730670880376515	28.980978838001548	19.699263450314756
28	18.75610605635837	33.34199862374799	29.50242543177783	18.39946988811581
29	19.260391976960523	32.585060019879194	28.409919208909955	19.744628794250325
30	20.156315042774253	32.380491203031156	28.976221423656245	18.486972330538354
31	20.53384985260511	33.338260655333826	26.926285563796075	19.201603928264987
32	20.666887546618412	32.08111391458742	28.02083067852622	19.231167860267945
33	20.22563736609153	32.87220395714929	27.975805149901028	18.926353526858154
34	19.233546567440595	32.82242101417879	29.776316571943152	18.16771584643746
35	20.37957370169313	32.11577507624606	29.69306182090034	17.811589401160468
36	20.71582095131296	33.59159296922123	28.446449354775677	17.246136724690132
37	20.339305587413243	32.754797767413415	29.28205520299717	17.62384144217618
38	20.058957956350724	32.566200270153175	30.05003780445328	17.324803969042826
39	20.259448989474222	31.751493063519977	28.772672052739335	19.216385894266466
40	21.67477975720196	32.55804470270408	28.30083849427836	17.4663370458156
41	19.803416842945857	31.938051668917943	29.49902727867404	18.759504209462158
42	20.935341641817672	31.951304466022716	28.789153095292708	18.3242007968669
43	19.65491755231032	32.865747466252095	28.906049562063018	18.573285419374567
44	21.065830721003135	31.749454171657703	27.848374408508974	19.336340698830185
45	20.742836268488077	32.836693257214705	27.415109887775994	19.005360586521224
46	18.997544834382513	33.33571204050598	28.677523765833268	18.98921935927823
47	19.99184443255091	32.413623195793086	28.95209453661935	18.642437835036656
48	20.183330359949366	32.1504362379047	29.57854406130268	18.08768934084325
49	21.009591287135443	31.66093228330402	28.35741774345643	18.972058686104102
50	19.025239782178385	32.309809618472364	28.6177162712066	20.047234328142654
51	20.02837457841663	30.439466150147393	29.074597956010905	20.457561315425068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6015.0
1	4687.0
2	3359.0
3	2260.5
4	1162.0
5	1137.5
6	1113.0
7	1415.0
8	1717.0
9	2191.5
10	2666.0
11	3430.0
12	4194.0
13	4249.5
14	4305.0
15	4162.5
16	4020.0
17	4068.0
18	4116.0
19	3773.0
20	3430.0
21	4020.5
22	4611.0
23	5045.0
24	5479.0
25	6750.5
26	8954.0
27	9886.0
28	11963.5
29	14041.0
30	16009.0
31	17977.0
32	21466.0
33	24955.0
34	26886.5
35	28818.0
36	30778.5
37	32739.0
38	34401.0
39	36063.0
40	37013.5
41	37964.0
42	38942.5
43	39921.0
44	41440.5
45	42960.0
46	43153.5
47	43347.0
48	45712.0
49	48077.0
50	45382.0
51	42687.0
52	37371.5
53	32056.0
54	28271.0
55	24486.0
56	20717.5
57	16949.0
58	14923.5
59	12898.0
60	11197.5
61	9497.0
62	8093.0
63	6689.0
64	5558.0
65	4427.0
66	3625.5
67	2824.0
68	2360.5
69	1897.0
70	1454.5
71	1012.0
72	917.0
73	822.0
74	680.5
75	460.5
76	382.0
77	273.0
78	164.0
79	153.5
80	143.0
81	104.0
82	65.0
83	40.5
84	16.0
85	13.0
86	10.0
87	9.0
88	8.0
89	7.5
90	7.0
91	8.0
92	9.0
93	7.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.221063674810395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04282507402563	22.804746926241915
2	9.603856374762621	5.99685222800395
3	4.0024563898782155	3.748828374121185
4	2.3143771085066054	2.890292602888332
5	1.635413206366641	2.5529669925299365
6	1.2124186545478666	2.2711800008500136
7	0.9297720425153863	2.0319930499701973
8	0.709379116460555	1.7718056451676578
9	0.5809762707452436	1.6324827428242021
>10	5.224964148093256	33.7721753541566
>50	0.5852408592606588	12.446158435881028
>100	0.1561370202878222	6.630701516996987
>500	5.459336373700078E-4	0.1704462885454986
>1k	0.0010918672747400155	0.3835041492273718
>5k	5.459336373700078E-4	0.8958656925951406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5256	0.8930346356755103	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	1196	0.20320955560652787	TruSeq Adapter, Index 15 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1054	0.1790826685696324	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1000	0.16990765518940457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.398153103788091E-4	0.0	0.0	0.24432720816236375	0.0
2	3.398153103788091E-4	0.0	0.0	0.36428201272608335	0.0
3	3.398153103788091E-4	0.0	0.0	0.48559607853131825	0.0
4	3.398153103788091E-4	0.0	0.0	1.0345677124482844	0.0
5	3.398153103788091E-4	0.0	0.0	1.1249585850090476	0.0
6	3.398153103788091E-4	0.0	0.0	1.7475002336230259	0.0
7	3.398153103788091E-4	0.0	0.0	2.2249407447052527	0.0
8	3.398153103788091E-4	0.0	0.0	2.712745622754033	0.0
9	3.398153103788091E-4	0.0	0.0	3.3347775484024433	0.0
10	3.398153103788091E-4	0.0	0.0	4.274876604565419	0.0
11	3.398153103788091E-4	0.0	0.0	5.1016472547170615	0.0
12	3.398153103788091E-4	0.0	0.0	5.510275165447579	0.0
13	3.398153103788091E-4	0.0	0.0	5.716373151192327	0.0
14	3.398153103788091E-4	0.0	0.0	5.912446585280899	0.0
15	3.398153103788091E-4	0.0	0.0	6.09220888447129	0.0
16	3.398153103788091E-4	0.0	0.0	6.4376311474713495	0.0
17	3.398153103788091E-4	0.0	0.0	6.907765629380432	0.0
18	3.398153103788091E-4	0.0	0.0	7.4803544273687255	0.0
19	3.398153103788091E-4	0.0	0.0	7.771915963673743	0.0
20	3.398153103788091E-4	0.0	0.0	8.065686299496225	0.0
21	3.398153103788091E-4	0.0	0.0	8.418244684014239	0.0
22	3.398153103788091E-4	0.0	0.0	8.792211433086118	0.0
23	3.398153103788091E-4	0.0	0.0	9.175353195538225	0.0
24	3.398153103788091E-4	0.0	0.0	9.457909626118205	0.0
25	3.398153103788091E-4	0.0	0.0	9.707334063936251	0.0
26	3.398153103788091E-4	0.0	0.0	9.945204781201417	0.0
27	3.398153103788091E-4	0.0	0.0	10.2180764754356	0.0
28	3.398153103788091E-4	0.0	0.0	10.490778262014596	0.0
29	3.398153103788091E-4	0.0	0.0	10.79746157963147	0.0
30	3.398153103788091E-4	0.0	0.0	11.212545981259186	0.0
31	3.398153103788091E-4	0.0	0.0	11.497311211356628	0.0
32	3.398153103788091E-4	0.0	0.0	11.79889729931782	0.0
33	3.398153103788091E-4	0.0	0.0	12.092667635140302	0.0
34	3.398153103788091E-4	0.0	0.0	12.379641664755205	0.0
35	3.398153103788091E-4	0.0	0.0	12.692781473269278	0.0
36	3.398153103788091E-4	0.0	0.0	12.99504719185123	0.0
37	3.398153103788091E-4	0.0	0.0	13.30869672333087	0.0
38	3.398153103788091E-4	0.0	0.0	13.640186558605398	0.0
39	3.398153103788091E-4	0.0	0.0	13.982890299122428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.2105738E-7	45.000004	1
ACTAGCG	35	1.2105738E-7	45.000004	1
GCGATGC	45	3.8380676E-10	45.000004	9
TCGAGCT	35	1.2105738E-7	45.000004	16
GCTACGA	35	1.2105738E-7	45.000004	9
CGGAACG	20	7.030478E-4	45.0	1
ACAGTCG	25	3.8884784E-5	45.0	1
CGAACCG	20	7.030478E-4	45.0	1
ATCTACG	20	7.030478E-4	45.0	1
GATCGCG	20	7.030478E-4	45.0	1
CGGCACG	20	7.030478E-4	45.0	1
CCTACGG	55	1.8189894E-12	45.0	2
TAACGCG	20	7.030478E-4	45.0	1
CCTCGCG	20	7.030478E-4	45.0	1
ATCCGTC	25	3.8884784E-5	45.0	44
CGGATTA	20	7.030478E-4	45.0	42
CGTAACG	40	6.8048394E-9	45.0	1
CGCAATG	25	3.8884784E-5	45.0	1
CACGTAC	20	7.030478E-4	45.0	20
GACGAGT	20	7.030478E-4	45.0	9
>>END_MODULE