FastQCFastQC Report
Sat 18 Jun 2016
SRR3554257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575231
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50920.8852095940587347No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT13040.2266915378343657TruSeq Adapter, Index 15 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.17975387279197402No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9510.1653248868715351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA302.1636024E-645.0000041
ACGCACG302.1636024E-645.0000041
TAAACCG453.8380676E-1045.0000041
CGCGCAG453.8380676E-1045.0000041
TATAGCG302.1636024E-645.0000041
CGCAACG453.8380676E-1045.0000041
GCCCGCG302.1636024E-645.0000041
AAACTCG207.0303463E-445.01
CGGGTAT800.045.06
CGGCGCG207.0303463E-445.01
ATCTCGC207.0303463E-445.010
CTATCCG207.0303463E-445.01
CGTGCAA207.0303463E-445.040
TAGACGG502.1827873E-1145.02
TAGACCG351.2105193E-745.01
CGTTGAC207.0303463E-445.032
GCGCCTA253.888367E-545.09
GTTAGTC351.2105193E-745.030
CGACACG207.0303463E-445.01
TATGCGG406.8048394E-945.02