##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554256_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 321841 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25114575209498 33.0 31.0 34.0 30.0 34.0 2 32.4364080399949 34.0 31.0 34.0 30.0 34.0 3 32.51014942160881 34.0 31.0 34.0 30.0 34.0 4 36.06364944180511 37.0 35.0 37.0 35.0 37.0 5 35.89060436675253 37.0 35.0 37.0 35.0 37.0 6 35.856528534276244 37.0 35.0 37.0 35.0 37.0 7 35.85226555970184 37.0 35.0 37.0 35.0 37.0 8 35.979098374663266 37.0 35.0 37.0 35.0 37.0 9 37.65573062474949 39.0 38.0 39.0 35.0 39.0 10 37.26162297532011 39.0 37.0 39.0 34.0 39.0 11 37.222995205707164 39.0 37.0 39.0 34.0 39.0 12 37.133556632001515 39.0 37.0 39.0 34.0 39.0 13 37.10271220882361 39.0 37.0 39.0 33.0 39.0 14 38.3352649289556 40.0 38.0 41.0 34.0 41.0 15 38.3727958836817 40.0 38.0 41.0 34.0 41.0 16 38.3562597680221 40.0 38.0 41.0 34.0 41.0 17 38.29699447864007 40.0 38.0 41.0 34.0 41.0 18 38.179175431346536 40.0 37.0 41.0 34.0 41.0 19 38.11178501185368 40.0 37.0 41.0 34.0 41.0 20 38.021420515099074 40.0 37.0 41.0 33.0 41.0 21 37.95317563641674 40.0 37.0 41.0 33.0 41.0 22 37.89137804070954 40.0 37.0 41.0 33.0 41.0 23 37.76285495011512 40.0 36.0 41.0 33.0 41.0 24 37.71678872486725 40.0 36.0 41.0 33.0 41.0 25 37.66617677673137 40.0 36.0 41.0 33.0 41.0 26 37.551853244303864 40.0 36.0 41.0 33.0 41.0 27 37.469244129865366 39.0 36.0 41.0 33.0 41.0 28 37.39405172119152 39.0 36.0 41.0 32.0 41.0 29 37.27671427816841 39.0 35.0 41.0 32.0 41.0 30 37.25786335488642 39.0 35.0 41.0 32.0 41.0 31 37.1584944118369 39.0 35.0 41.0 32.0 41.0 32 37.05535341985639 39.0 35.0 41.0 31.0 41.0 33 36.97112238652005 39.0 35.0 41.0 31.0 41.0 34 36.8164186663601 39.0 35.0 41.0 31.0 41.0 35 36.736863855133436 39.0 35.0 41.0 31.0 41.0 36 36.63299268893646 39.0 35.0 41.0 31.0 41.0 37 36.58455262070401 39.0 35.0 41.0 30.0 41.0 38 36.46509922601533 39.0 35.0 41.0 30.0 41.0 39 36.402950525259364 39.0 35.0 41.0 30.0 41.0 40 36.31797067496062 39.0 35.0 40.0 30.0 41.0 41 36.2067698024801 39.0 35.0 40.0 30.0 41.0 42 36.08467845923919 38.0 35.0 40.0 30.0 41.0 43 35.95253867593004 38.0 35.0 40.0 29.0 41.0 44 35.82108245997247 38.0 35.0 40.0 29.0 41.0 45 35.63506514086148 38.0 35.0 40.0 28.0 41.0 46 35.51144198532816 38.0 34.0 40.0 28.0 41.0 47 35.306750849021725 38.0 34.0 40.0 27.0 41.0 48 35.24280312328137 38.0 34.0 40.0 27.0 41.0 49 35.09616860499439 37.0 34.0 40.0 27.0 41.0 50 34.93350753943717 37.0 34.0 40.0 26.0 41.0 51 33.904996566627624 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 3.0 13 2.0 14 8.0 15 23.0 16 31.0 17 47.0 18 122.0 19 223.0 20 386.0 21 603.0 22 910.0 23 1177.0 24 1637.0 25 1886.0 26 2296.0 27 2838.0 28 3563.0 29 4505.0 30 5761.0 31 7332.0 32 9431.0 33 12707.0 34 19386.0 35 27196.0 36 28475.0 37 40401.0 38 65381.0 39 85478.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.862220786040314 30.23014469877983 21.218241305489357 25.689393209690497 2 25.643718482107623 32.743186853135555 18.44171500834263 23.171379656414192 3 19.774671343924485 33.57403189773833 20.646219717189542 26.005077041147644 4 20.86061129563977 33.92575837136971 20.230486482455625 24.98314385053489 5 18.6666708094991 36.50467156142319 16.637718625035344 28.19093900404237 6 20.32556448681181 39.52479640567858 19.099182515589995 21.050456591919612 7 82.17473845780991 8.524395586640608 4.698904117250444 4.601961838299036 8 83.68511159237015 8.03346994323284 4.682436358326006 3.59898210607101 9 76.32930546449956 11.763572695834279 6.900301701771994 5.006820137894177 10 33.83658390323172 36.30612631703232 14.860443510926203 14.996846268809755 11 25.469719519887153 31.800174620387082 24.487557520639072 18.242548339086692 12 23.263350536445017 29.886496748394393 29.08983007137065 17.760322643789948 13 24.115013314027735 30.540235706451323 25.39701281067359 19.94773816884735 14 20.905353885925038 29.826529248914834 28.286017008398556 20.982099856761568 15 19.450908989221386 30.33858333773509 29.147933296254987 21.062574376788536 16 21.140873909787754 31.528611954350126 26.85798266846051 20.472531467401605 17 21.217619880624284 30.29259789771968 27.242023235075706 21.24775898658033 18 23.18940097750131 29.79949726728416 26.08058016225403 20.9305215929605 19 21.924179952212427 31.246174353174393 25.88358848002585 20.946057214587327 20 24.65409938447867 28.761096317746965 26.674040908398865 19.9107633893755 21 24.069649298877398 31.612815023567535 25.043422062446986 19.27411361510808 22 24.200148520542754 29.257925497372927 25.488051553406805 21.053874428677517 23 21.51155384180387 31.73088574793143 26.30149670178753 20.456063708477167 24 19.40896281082895 31.289363381296976 28.970516497276606 20.33115731059747 25 21.111356228696778 32.344232089758606 25.28950630901594 21.254905372528672 26 23.25278631373877 30.899729990896123 25.45946600961344 20.38801768575166 27 21.288773027675155 30.042474389527747 25.652729142651186 23.01602344014591 28 20.569784458785552 31.9617450853061 26.719715635981743 20.74875481992661 29 21.566549942362844 31.678996771697825 25.456358885288076 21.298094400651255 30 22.720846629236174 29.275014681162435 27.75159162443567 20.252547065165718 31 22.672686202193006 30.463179023182253 25.6604969534646 21.203637821160136 32 22.290509910173036 30.413154321543868 26.741154793826766 20.55518097445633 33 21.52988587532353 29.870961126767565 26.422053125611715 22.17709987229719 34 21.261430333611937 30.529982196177613 27.691313412523577 20.517274057686873 35 20.829229339953578 31.144882100167475 28.802110358841787 19.22377820103716 36 21.181887950882576 33.688684785344314 25.713007354563278 19.41641990920983 37 20.65585180259818 31.839013674454158 26.092387234690424 21.412747288257243 38 20.879254041591967 32.00835195018658 27.05373150095855 20.058662507262902 39 20.888264702135526 30.3522546847667 26.933175077134365 21.82630553596341 40 22.730168002212274 30.08659555494794 26.245879176363484 20.937357266476305 41 19.544744143847428 30.242573196081295 28.429255439797913 21.783427220273367 42 23.08406946287142 29.44994578068052 26.390981882358062 21.07500287409 43 22.50738718808356 29.924403665163858 26.00228062925482 21.56592851749777 44 23.78006531175332 29.36325701200282 25.73662149943606 21.120056176807804 45 22.559586876749698 30.20559841660944 26.542609549435902 20.692205157204953 46 19.99030577210486 29.533838137465395 28.568143897141756 21.90771219328799 47 20.79722595940231 30.928936959554566 27.98928663532614 20.284550445716985 48 21.749248852694343 29.768426024030497 28.537694078753173 19.944631044521984 49 22.04784350036198 30.293840747449828 27.37221174430853 20.28610400787967 50 18.65859228625315 31.90177758582654 27.6040032189808 21.835626908939506 51 20.029455538604466 28.872020656162515 29.256993360075317 21.8415304451577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6559.0 1 5080.5 2 3602.0 3 2273.5 4 945.0 5 778.5 6 612.0 7 641.0 8 670.0 9 699.5 10 729.0 11 877.5 12 1026.0 13 1056.5 14 1087.0 15 1217.0 16 1347.0 17 1232.5 18 1118.0 19 1114.0 20 1110.0 21 1219.5 22 1329.0 23 1846.0 24 2363.0 25 2534.5 26 3147.0 27 3588.0 28 4711.0 29 5834.0 30 6517.5 31 7201.0 32 8727.5 33 10254.0 34 12153.0 35 14052.0 36 14863.5 37 15675.0 38 15061.5 39 14448.0 40 15434.5 41 16421.0 42 16746.5 43 17072.0 44 17515.5 45 17959.0 46 19020.0 47 20081.0 48 21788.5 49 23496.0 50 22763.5 51 22031.0 52 21646.0 53 21261.0 54 19050.5 55 16840.0 56 15593.5 57 14347.0 58 12876.5 59 11406.0 60 10674.0 61 9942.0 62 9623.5 63 9305.0 64 8096.0 65 6887.0 66 5994.5 67 5102.0 68 4775.0 69 4448.0 70 3737.5 71 3027.0 72 2632.5 73 2238.0 74 1958.5 75 1281.5 76 884.0 77 719.5 78 555.0 79 407.0 80 259.0 81 225.0 82 191.0 83 131.5 84 72.0 85 49.0 86 26.0 87 28.5 88 31.0 89 24.5 90 18.0 91 10.5 92 3.0 93 3.0 94 3.0 95 2.0 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 321841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.32865607551555 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28109129272063 19.574261824938404 2 7.571333938516648 3.835434267231335 3 2.714737849309355 2.0628198396102424 4 1.4929218086802916 1.5125481215879892 5 1.0770627346107609 1.3640275788355118 6 0.744620819941608 1.1316146792981627 7 0.6060011285850978 1.0744435917114352 8 0.5238106921170784 1.0613936695448993 9 0.495596064672833 1.1297504047029434 >10 6.223165435854658 35.259336131816646 >50 0.8991879094187787 16.180660636774057 >100 0.36433671091047376 12.94490136433829 >500 0.002453445864716995 0.40392616229753203 >1k 0.002453445864716995 0.7429134261949223 >5k 0.0012267229323584975 1.7219683011176328 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5542 1.7219683011176328 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1223 0.3800013049922167 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1168 0.36291212120270566 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 686 0.21314872872008225 No Hit CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT 614 0.19077743357744972 No Hit CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC 375 0.11651716220121115 No Hit ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA 326 0.10129225300691956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.22588793845408137 0.0 2 0.0 0.0 0.0 0.31009100767148995 0.0 3 0.0 0.0 0.0 0.4064118617578245 0.0 4 0.0 0.0 0.0 0.8445163916343784 0.0 5 0.0 0.0 0.0 0.9066588781416911 0.0 6 0.0 0.0 0.0 1.3730382393790723 0.0 7 0.0 0.0 0.0 1.7350182232841682 0.0 8 0.0 0.0 0.0 2.102901743407459 0.0 9 0.0 0.0 0.0 2.545977672204598 0.0 10 0.0 0.0 0.0 3.274598326502838 0.0 11 0.0 0.0 0.0 3.8282878812829937 0.0 12 0.0 0.0 0.0 4.100471972185023 0.0 13 0.0 0.0 0.0 4.206114199247454 0.0 14 0.0 0.0 0.0 4.3220099365835924 0.0 15 0.0 0.0 0.0 4.4605876814949 0.0 16 0.0 0.0 0.0 4.711021902119369 0.0 17 0.0 0.0 0.0 5.013034386544909 0.0 18 0.0 0.0 0.0 5.415406986679758 0.0 19 0.0 0.0 0.0 5.567656078622674 0.0 20 0.0 0.0 0.0 5.770240584636513 0.0 21 0.0 0.0 0.0 5.983389313356596 0.0 22 0.0 0.0 0.0 6.2201521869494565 0.0 23 0.0 0.0 0.0 6.452254374054269 0.0 24 0.0 0.0 0.0 6.630914022762793 0.0 25 0.0 0.0 0.0 6.781920264975563 0.0 26 0.0 0.0 0.0 6.930130095295503 0.0 27 0.0 0.0 0.0 7.085175599131248 0.0 28 0.0 3.107124325365631E-4 0.0 7.244260364589969 0.0 29 0.0 3.107124325365631E-4 0.0 7.423541438163565 0.0 30 0.0 3.107124325365631E-4 0.0 7.685782731224425 0.0 31 0.0 3.107124325365631E-4 0.0 7.8597816934449 0.0 32 0.0 3.107124325365631E-4 0.0 8.035334217828058 0.0 33 3.107124325365631E-4 3.107124325365631E-4 0.0 8.214304578969118 0.0 34 3.107124325365631E-4 3.107124325365631E-4 0.0 8.39576063957047 0.0 35 3.107124325365631E-4 3.107124325365631E-4 0.0 8.581255961794799 0.0 36 3.107124325365631E-4 3.107124325365631E-4 0.0 8.752769224554982 0.0 37 3.107124325365631E-4 3.107124325365631E-4 0.0 8.940128821374529 0.0 38 3.107124325365631E-4 3.107124325365631E-4 0.0 9.152656125229539 0.0 39 3.107124325365631E-4 3.107124325365631E-4 0.0 9.347162107997427 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGCG 65 0.0 45.000004 5 CGTACTG 45 3.8380676E-10 45.000004 1 CTGTCGT 20 7.025709E-4 45.0 37 CTCACGG 50 2.1827873E-11 45.0 2 TCCATCG 20 7.025709E-4 45.0 36 GTCGCAT 20 7.025709E-4 45.0 25 ATCTCAC 20 7.025709E-4 45.0 32 CGTATTC 20 7.025709E-4 45.0 24 AATCACG 20 7.025709E-4 45.0 1 GATCGCA 20 7.025709E-4 45.0 34 TACCACG 20 7.025709E-4 45.0 1 CCGATTT 20 7.025709E-4 45.0 18 ACGTTTA 20 7.025709E-4 45.0 35 CGAAACG 20 7.025709E-4 45.0 1 CGGCCGA 20 7.025709E-4 45.0 29 GTTTACG 20 7.025709E-4 45.0 16 CATGACG 20 7.025709E-4 45.0 30 TAGCGTT 35 1.2082819E-7 45.0 17 GACCGAG 25 3.8845254E-5 45.0 16 CAACCCA 20 7.025709E-4 45.0 3 >>END_MODULE