FastQCFastQC Report
Sat 18 Jun 2016
SRR3554254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132241
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31160.2752064269002801No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT12610.11137204888358573No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG253.890769E-545.01
GTCGCTC207.033243E-445.028
GAGTACG207.033243E-445.01
CGTTATC207.033243E-445.038
TGTTACG253.890769E-545.01
CGCGTAA207.033243E-445.031
TCAACCG551.8189894E-1245.01
ATATCCG1150.043.043481
AATTGCG950.042.631581
TACACCG850.042.352941
CGTACCG603.6379788E-1241.2500041
AGTACGG1700.041.029412
TAATCCG556.184564E-1140.9090921
TCACGCG1000.040.51
CTAACGG1900.040.263162
TAACCCG451.9283107E-840.0000041
TAGTACG451.9283107E-840.0000041
ATATGCG403.4581535E-739.3751
TGTAGCG403.4581535E-739.3751
CAATGCG403.4581535E-739.3751