FastQCFastQC Report
Sat 18 Jun 2016
SRR3554253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106400
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31220.28217642805495297No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT13180.11912509038322487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGT207.033171E-445.0000043
CGCGGAT406.8139343E-945.00000423
TGCGATG207.033171E-445.0000041
TATAGCG207.033171E-445.0000041
CGCGTTG207.033171E-445.0000041
TCGAACG207.033171E-445.0000041
TAGTACG207.033171E-445.0000041
TCCGACG253.8907107E-545.01
GTGATCG253.8907107E-545.015
CCATGCG453.8562575E-1045.01
AATTGCG1050.045.01
GCGGAAT351.2118653E-745.05
CAGTACG700.045.01
CCACGCG700.041.7857131
ATTGCGG2550.041.470592
AAACGCG603.6379788E-1241.2499961
GCTAGCG556.184564E-1140.9090921
TGTAGCG501.0822987E-940.51
TACACCG501.0822987E-940.51
CATTGCG900.040.01